公开(公告)号：AT123062T

公开(公告)日：1995-06-15

申请号：AT88117742

申请日：1988-10-25

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  RAPPUOLI RINO ,  BARTOLONI ANTONELLA

IPC: A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/20 ,  C12N1/21 ,  C12N15/09 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12N15/31

Abstract: Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating a microorganismtransformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.

公开(公告)号：MX20502A

公开(公告)日：1994-02-28

申请号：MX2050290

申请日：1990-04-27

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA

IPC: A61K39/10

公开(公告)号：IT1230139B

公开(公告)日：1991-10-14

申请号：IT2034189

申请日：1989-04-28

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO

IPC: A61K39/10 ,  C12K

Abstract: The toxin mutants (A) have at least one of the amino acid (AA) residues Glu129, Asp11, Trp26, Arg9, Phe50, Asp1, Arg13, Tyr130, Gly86, Ile88, Tyr89, Tyr8, Gly44, Thr53 and Gly80 of subunit S1 deleted and substd. by a natural AA. Also claimed are (a) Bordetella strains comprising (i) the gene encoding the wild type pertussin toxin; (ii) an expression and secretion regulation sequence; with the mutated subunit S1. E.g. Bordetella pertussis (W28)PT-9K/129G ATCC53894; B.parapertussis PT-129G ATCC 53892, PT-26l/129G ATCC 53893; B.bronchiseptica; (b) an immunogenic formulation. Details for obtg. (A) and Bordetella strains are given. Six specific examples of (A) are given, e.g. PT-13l/129G and PT-88E/89S have AA Glu129 + Arg13 and Ile88 + Tyr89 substd. by Gly+Ile and Glu+Ser respectively. (A), stable at 37 deg.C, have reduced or no mitogenicity of haemagglutinin activity and are obtd. by 0.035% and 0.42% formaldehyde treatment.

公开(公告)号：IT8723085D0

公开(公告)日：1987-12-18

申请号：IT2308587

申请日：1987-12-18

Applicant: SCLAVO SPA

Inventor： BARTOLONI ANTONELLA ,  PIZZA MARIAGRAZIA ,  RAPPUOLI RINO

IPC: G01N33/569 ,  A61K39/00 ,  A61K39/12 ,  C07K14/235 ,  C12N1/19 ,  C12N1/21 ,  C12P21/02 ,  C12R1/07 ,  C12R1/19 ,  C12R1/645