公开(公告)号：GR3018285T3

公开(公告)日：1996-03-31

申请号：GR950403403

申请日：1995-12-05

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO ,  NENCIONI LUCIANO

IPC: C12N1/20 ,  A61K39/00 ,  A61K39/10 ,  C07K14/235 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/31 ,  C12R1/01

公开(公告)号：AT127347T

公开(公告)日：1995-09-15

申请号：AT90107892

申请日：1990-04-25

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO ,  CALAMANDREI ,  NENCIONI LUCIANO

IPC: C12N1/20 ,  A61K39/00 ,  A61K39/10 ,  C07K14/235 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/31 ,  C12R1/01

公开(公告)号：IT1238007B

公开(公告)日：1993-06-21

申请号：IT1928690

申请日：1990-02-07

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  NENCIONI LUCIANO ,  RAPPUOLI RINO

IPC: C12K

Abstract: The toxin mutants (A) have at least one of the amino acid (AA) residues Glu129, Asp11, Trp26, Arg9, Phe50, Asp1, Arg13, Tyr130, Gly86, Ile88, Tyr89, Tyr8, Gly44, Thr53 and Gly80 of subunit S1 deleted and substd. by a natural AA. Also claimed are (a) Bordetella strains comprising (i) the gene encoding the wild type pertussin toxin; (ii) an expression and secretion regulation sequence; with the mutated subunit S1. E.g. Bordetella pertussis (W28)PT-9K/129G ATCC53894; B.parapertussis PT-129G ATCC 53892, PT-26l/129G ATCC 53893; B.bronchiseptica; (b) an immunogenic formulation. Details for obtg. (A) and Bordetella strains are given. Six specific examples of (A) are given, e.g. PT-13l/129G and PT-88E/89S have AA Glu129 + Arg13 and Ile88 + Tyr89 substd. by Gly+Ile and Glu+Ser respectively. (A), stable at 37 deg.C, have reduced or no mitogenicity of haemagglutinin activity and are obtd. by 0.035% and 0.42% formaldehyde treatment.

公开(公告)号：IT9019286A1

公开(公告)日：1991-08-08

申请号：IT1928690

申请日：1990-02-07

Applicant: SCLAVO SPA

Inventor： COVACCI ANTONELLO ,  NENCIONI LUCIANO ,  PIZZA MARIAGRAZIA ,  RAPPUOLI RINO

公开(公告)号：IT1223529B

公开(公告)日：1990-09-19

申请号：IT2308587

申请日：1987-12-18

Applicant: SCLAVO SPA

Inventor： BARTOLONI ANTONELLA ,  PIZZA MARIAGRAZIA ,  RAPPUOLI RINO

IPC: G01N33/569 ,  A61K39/00 ,  A61K39/12 ,  C07K14/235 ,  C12N1/19 ,  C12N1/21 ,  C12P21/02 ,  C12R1/07 ,  C12R1/19 ,  C12R1/645 ,  A61K

公开(公告)号：IT1223334B

公开(公告)日：1990-09-19

申请号：IT2248187

申请日：1987-11-02

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  BARTOLONI ANTONELLA ,  RAPPUOLI RINO

IPC: A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/20 ,  C12N1/21 ,  C12N15/09 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  A61K

Abstract: Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating a microorganismtransformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.

公开(公告)号：IT9019286D0

公开(公告)日：1990-02-07

申请号：IT1928690

申请日：1990-02-07

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  NENCIONI LUCIANO ,  RAPPUOLI RINO

IPC: C12K

公开(公告)号：IT8722481D0

公开(公告)日：1987-11-02

申请号：IT2248187

申请日：1987-11-02

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  BARTOLONI ANTONELLA ,  RAPPUOLI RINO

IPC: A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/20 ,  C12N1/21 ,  C12N15/09 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

Abstract: Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating a microorganismtransformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.

公开(公告)号：CA1341582C

公开(公告)日：2008-10-07

申请号：CA581889

申请日：1988-11-01

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  RAPPUOLI RINO ,  BARTOLONI ANTONELLA

IPC: C12N15/32 ,  A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/20 ,  C12N1/21 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

Abstract: Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating a microorganismtransformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.

公开(公告)号：ES2078258T3

公开(公告)日：1995-12-16

申请号：ES90107892

申请日：1990-04-25

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO ,  NENCIONI LUCIANO

IPC: C12N1/20 ,  A61K39/00 ,  A61K39/10 ,  C07K14/235 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/31 ,  C12R1/01