公开(公告)号：AU2009293073A1

公开(公告)日：2010-03-25

申请号：AU2009293073

申请日：2009-09-18

Applicant: SINGULEX INC

Inventor： TODD JOHN

IPC: G01N33/537 ,  G01N33/543

Abstract: The present invention provides single molecule analyses of species of use in analytical, diagnostic or prognostic assays. In exemplary embodiments, the assays utilize samples prepared by novel methods, affording assays of unexpected sensitivity and robustness. The method is described in a non-limiting manner by reference to cytokine assays.

公开(公告)号：CA2648382A1

公开(公告)日：2008-04-24

申请号：CA2648382

申请日：2007-04-04

Applicant: SINGULEX INC

Inventor： TODD JOHN ,  PUSKAS ROBERT ,  GOIX PHILIPPE ,  LIVINGSTON RICHARD ,  HELD DOUGLAS

IPC: G01N21/76 ,  G01N21/64

Abstract: Disclosed are methods, kits, and compositions for fluorescent detection of single biomolecules, e.g. proteins, using highly sensitive fluorescent dyes and a flow cell with one interrogation space for irradiation by focused lase r beam. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomola r, or lower. The methods, kits, and compositions also allow the determination o f concentration over a wide range, e.g., 7-log range, without need for sample dilution.

公开(公告)号：EP2002260A4

公开(公告)日：2009-08-05

申请号：EP07754941

申请日：2007-04-04

Applicant: SINGULEX INC

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS

IPC: G01N33/53

CPC classification number: G01N33/6887 ,  G01N15/06 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/577 ,  G01N33/582 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2201/1247 ,  G01N2201/12761 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/52 ,  Y10T436/105831

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

Abstract translation: 本发明提供用于敏感检测心肌肌钙蛋白的方法，组合物，试剂盒和系统。 这些方法，组合物，试剂盒和系统可用于涉及心肌肌钙蛋白释放的病症中的治疗方法的诊断，预后和测定。

公开(公告)号：EP2440936A4

公开(公告)日：2013-03-13

申请号：EP10786631

申请日：2010-06-07

Applicant: SINGULEX INC

Inventor： GOIX PHILIPPE J ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  LE SARA

IPC: G01N33/68 ,  G01N33/52

CPC classification number: G01N33/6869 ,  G01N33/6893 ,  G01N2333/32 ,  G01N2333/47 ,  G01N2333/4712 ,  G01N2333/525 ,  G01N2333/54 ,  G01N2333/5412 ,  G01N2333/58 ,  G01N2800/32 ,  G01N2800/325 ,  G01N2800/60

公开(公告)号：EP2331960A4

公开(公告)日：2012-02-01

申请号：EP09815292

申请日：2009-09-18

Applicant: SINGULEX INC

Inventor： TODD JOHN

IPC: G01N33/537 ,  G01N33/543

CPC classification number: G01N33/6827 ,  G01N33/54333 ,  G01N33/6869 ,  G01N33/74 ,  G01N2333/46 ,  G01N2333/4712 ,  G01N2333/475 ,  G01N2333/525 ,  G01N2333/535 ,  G01N2333/54 ,  G01N2333/5412 ,  G01N2333/5418 ,  G01N2333/545 ,  G01N2333/555

公开(公告)号：AU2016204877A1

公开(公告)日：2016-07-28

申请号：AU2016204877

申请日：2016-07-13

Applicant: SINGULEX INC ,  UNIV CALIFORNIA

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  WU ALAN

IPC: G01N33/53

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin. WO 2007/114947 PCT/US2007/008506 30 3 7 0 630 5 Figure 1A

公开(公告)号：ES2550004T3

公开(公告)日：2015-11-03

申请号：ES11169710

申请日：2007-04-04

Applicant: SINGULEX INC ,  UNIV CALIFORNIA

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  WU ALAN H

IPC: G01N33/53 ,  G01N21/64 ,  G01N33/58 ,  G01N33/68

Abstract: Un método para determinar una enfermedad cardiovascular en un individuo que comprende: (a) medir la concentración en suero o plasma de la troponina cardiaca a partir de una muestra de sangre del individuo; (b) comparar la concentración medida a una concentración umbral predeterminada que representa la concentración de percentil 99º de la troponina cardiaca en un grupo de individuos normales con un coeficiente de variación (CV) correspondiente del 10% o menos, en donde la concentración umbral es menos de 10 pg/mL; (c) determinar al menos uno de los siguientes en el individuo cuando la concentración de troponina cardiaca es mayor que la concentración umbral: daño cardiaco, daño miocárdico, un evento cardiaco anterior y cardiotoxicidad.

公开(公告)号：AU2013219225A1

公开(公告)日：2013-10-03

申请号：AU2013219225

申请日：2013-08-23

Applicant: SINGULEX INC

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS

IPC: G01N21/64 ,  G01N21/76

Abstract: METHODS AND COMPOSITIONS FOR HIGHLY SENSITIVE ANALYSIS OF MARKERS AND DETECTION OF MOLECULES 5OF THE DISCLOSURE Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution. WO 2008/048371 PCT/US2007/008394 Figur IA1 308 307 306 305 Figure 1A

公开(公告)号：GB2464183A

公开(公告)日：2010-04-14

申请号：GB0916302

申请日：2009-09-17

Applicant: SINGULEX INC

Inventor： TODD JOHN ,  LU QUYNHANH ,  FREESE ROBERT

IPC: G01N33/543 ,  G01N33/58

Abstract: An assay for detecting a detectable species comprising forming a complex between an analyte and a capture species, wherein said capture species is immobilized on a magnetic particle; contacting said complex with a detectable species which specifically binds to said analyte thereby forming an immobilized complex; transferring said immobilized complex to another vessel free of said detectable species; eluting said detectable species from said immobilized complex and detecting said detectable species. Also claimed is a dataset from an assay comprising at least one data point corresponding to a signal from an analyte collected from an assay.

公开(公告)号：CA2737482A1

公开(公告)日：2010-03-25

申请号：CA2737482

申请日：2009-09-18

Applicant: SINGULEX INC

Inventor： TODD JOHN

IPC: G01N33/537 ,  G01N33/543

Abstract: Single molecule analysis of species of use in analytical, diagnostic or prognostic assays is disclosed In exemplary embodiments, the assays utilize samples prepared by the disclosed methods, affording assays of unexpected sensitivity and robustness The method is described in a non-limiting manner by reference to cytokine assays