公开(公告)号：WO2009126380A8

公开(公告)日：2010-11-04

申请号：PCT/US2009036086

申请日：2009-03-04

Applicant: SINGULEX INC ,  GOIX PHILIPPE J ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD A ,  HELD DOUGLAS ,  AGEE SARA

Inventor： GOIX PHILIPPE J ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD A ,  HELD DOUGLAS ,  AGEE SARA

IPC: G01N33/53

CPC classification number: G01N33/577 ,  A61B5/00 ,  A61B5/0402 ,  A61B5/412 ,  A61B5/415 ,  A61B5/418 ,  A61B5/7264 ,  A61B5/7275 ,  G01N21/6428 ,  G01N33/582 ,  G01N33/6863 ,  G01N33/6869 ,  G01N33/6872 ,  G01N33/6887 ,  G01N33/6896 ,  G01N33/74 ,  G01N33/92 ,  G01N2333/4712 ,  G01N2333/475 ,  Y02A90/26 ,  Y10T436/143333

Abstract: The present invention discloses methods for the detection and monitoring of a condition in a subject using highly sensitive detection of molecules. The invention provides a method for detecting or monitoring a condition in a subject, comprising detecting a first marker in a first sample from the subject and detecting a second marker, wherein the first marker comprises a biomarker, e.g., Cardiac Troponin-I (cTnl) or Vascular Endothelial Growth Factor (VEGF), and wherein the limit of detection of the first marker is less than about 10 pg/ml. The second marker can be a biomarker, physiological marker, a molecular marker or a genetic marker.

Abstract translation: 本发明公开了使用分子的高灵敏度检测来检测和监测受试者的状况的方法。 本发明提供了一种用于检测或监测受试者中的病症的方法，包括检测来自受试者的第一样品中的第一标志物并检测第二标志物，其中第一标记物包含生物标志物，例如心肌肌钙蛋白I（cTn1） 或血管内皮生长因子（VEGF），并且其中第一标记物的检测限小于约10pg / ml。 第二标记可以是生物标志物，生理学标记，分子标记或遗传标记。

公开(公告)号：CA2648385C

公开(公告)日：2020-09-01

申请号：CA2648385

申请日：2007-04-04

Applicant: SINGULEX INC ,  UNIV CALIFORNIA

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  WU ALAN H

IPC: G01N33/53

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

公开(公告)号：AU2007313488A1

公开(公告)日：2008-04-24

申请号：AU2007313488

申请日：2007-04-04

Applicant: SINGULEX INC

Inventor： PUSKAS ROBERT ,  HELD DOUGLAS ,  GOIX PHILIPPE ,  LIVINGSTON RICHARD ,  TODD JOHN

IPC: B01J19/00 ,  C40B30/04 ,  G01N21/63 ,  G01N33/566 ,  G01N33/68 ,  G01N35/10

Abstract: The invention provides methods for diagnosing, predicting or prognosing acute myocardial infarction in an individual based on the sensitive detection of cardiac troponin.

公开(公告)号：ES2560864T3

公开(公告)日：2016-02-23

申请号：ES07754941

申请日：2007-04-04

Applicant: SINGULEX INC ,  UNIV CALIFORNIA

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  WU ALAN H

IPC: G01N33/53

Abstract: Un método para determinar la presencia o ausencia de una única molécula de troponina, o un fragmento o complejo de la misma, en una muestra, que comprende: i) marcar dicha molécula, fragmento o complejo con una etiqueta que comprende una unidad estructural fluorescente; y ii) detectar la presencia o ausencia de dicha etiqueta haciendo pasar dicha etiqueta a través de una única molécula de detección, en donde la detección de la presencia de dicha etiqueta indica la presencia de dicha única molécula, fragmento o complejo de troponina en dicha muestra; y en donde dicha detección es capaz de detectar dicha única molécula de troponina en un límite de detección de menos 3 pg/mL con un coeficiente de variación (CV) de 10% o menos.

公开(公告)号：CA2762612A1

公开(公告)日：2010-12-16

申请号：CA2762612

申请日：2010-06-07

Applicant: SINGULEX INC

Inventor： GOIX PHILIPPE J ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  LE SARA

IPC: G01N33/68 ,  G01N33/52

Abstract: Cardiovascular disease, e.g., congestive heart failure, is often first diagnosed after the onset of clinical symptoms, eliminating potential for early intervention. The invention provides a multi-marker immunoassay, including cardiac pathology and vascular inflammation biomarkers, yielding a more sensitive assay for early detection of CHF in plasma. A panel consisting of cardiac pathology (cTnl, BNP) and vascular inflammation (IL-6, TNFa, IL- 17a) biomarkers provided a sensitivity of 94% for association with CHF.

公开(公告)号：WO2008048371A2

公开(公告)日：2008-04-24

申请号：PCT/US2007008394

申请日：2007-04-04

Applicant: SINGULEX INC ,  GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS

IPC: B01J19/00 ,  C40B30/04 ,  G01N21/63 ,  G01N33/566 ,  G01N33/68 ,  G01N35/10

CPC classification number: G01N33/6887 ,  G01N15/06 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/577 ,  G01N33/582 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2201/1247 ,  G01N2201/12761 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/52 ,  Y10T436/105831

Abstract: Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

Abstract translation: 公开了用于分子的高灵敏度检测的方法，试剂盒和组合物。 方法，试剂盒和组合物可用于测定样品中分子的浓度为1飞摩尔，1 attomolar或更低的水平。 方法，试剂盒和组合物还允许在宽范围内测定浓度，例如7-log范围，而不需要样品稀释。

公开(公告)号：WO2009126380A3

公开(公告)日：2009-12-03

申请号：PCT/US2009036086

申请日：2009-03-04

Applicant: SINGULEX INC ,  GOIX PHILIPPE J ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD A ,  HELD DOUGLAS ,  AGEE SARA

Inventor： GOIX PHILIPPE J ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD A ,  HELD DOUGLAS ,  AGEE SARA

IPC: G01N33/53

CPC classification number: G01N33/577 ,  A61B5/00 ,  A61B5/0402 ,  A61B5/412 ,  A61B5/415 ,  A61B5/418 ,  A61B5/7264 ,  A61B5/7275 ,  G01N21/6428 ,  G01N33/582 ,  G01N33/6863 ,  G01N33/6869 ,  G01N33/6872 ,  G01N33/6887 ,  G01N33/6896 ,  G01N33/74 ,  G01N33/92 ,  G01N2333/4712 ,  G01N2333/475 ,  Y02A90/26 ,  Y10T436/143333

Abstract: The present invention discloses methods for the detection and monitoring of a condition in a subject using highly sensitive detection of molecules. The invention provides a method for detecting or monitoring a condition in a subject, comprising detecting a first marker in a first sample from the subject and detecting a second marker, wherein the first marker comprises a biomarker, e.g., Cardiac Troponin-I (cTnl) or Vascular Endothelial Growth Factor (VEGF), and wherein the limit of detection of the first marker is less than about 10 pg/ml. The second marker can be a biomarker, physiological marker, a molecular marker or a genetic marker.

Abstract translation: 本发明公开了使用分子的高灵敏度检测来检测和监测受试者状况的方法。 本发明提供了用于检测或监测受试者状况的方法，包括检测来自受试者的第一样品中的第一标志物和检测第二标志物，其中第一标志物包含生物标志物，例如心脏肌钙蛋白-I（cTnI） 或血管内皮生长因子（VEGF），并且其中第一标记的检测极限小于约10pg / ml。 第二个标记可以是生物标记，生理标记，分子标记或遗传标记。

公开(公告)号：CA2648382C

公开(公告)日：2016-10-11

申请号：CA2648382

申请日：2007-04-04

Applicant: SINGULEX INC

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS

IPC: G01N21/76 ,  G01N21/64

Abstract: Disclosed are methods, kits, and compositions for fluorescent detection of single biomolecules, e.g. proteins, using highly sensitive fluorescent dyes and a flow cell with one interrogation space for irradiation by focused laser beam. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

公开(公告)号：AU2014200749B2

公开(公告)日：2016-04-14

申请号：AU2014200749

申请日：2014-02-12

Applicant: SINGULEX INC ,  UNIV CALIFORNIA

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  WU ALAN

IPC: G01N33/53

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin. WO 2007/114947 PCT/US2007/008506 30 3 7 0 630 5 Figure 1A

公开(公告)号：HK1163818A1

公开(公告)日：2012-09-14

申请号：HK12104461

申请日：2012-05-08

Applicant: SINGULEX INC

Inventor： GOIX PHILIPPE ,  PUSKAS ROBERT ,  TODD JOHN ,  LIVINGSTON RICHARD ,  HELD DOUGLAS ,  WU ALAN H

IPC: G01N20060101

Abstract: The invention provides methods for diagnosing, predicting or prognosing acute myocardial infarction in an individual based on the sensitive detection of cardiac troponin.