公开(公告)号：EP2891721A4

公开(公告)日：2016-04-06

申请号：EP13832897

申请日：2013-08-29

Applicant: TORAY INDUSTRIES

Inventor： HIRANO TAISUKE ,  NAKAMURA FUMIO ,  UEDA YOJI ,  FUJIMOTO KENZO

IPC: C12Q1/68 ,  C12N15/09

CPC classification number: C12Q1/6825 ,  C12Q1/6834 ,  C12Q2523/313 ,  C12Q2537/125 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, which enables highly sensitive detection of the target nucleic acid without lowering the detection sensitivity (S/N ratio) even by use of a reduced amount of a detection probe, is disclosed. The method for detecting a target nucleic acid is based on sandwich hybridization using a detection probe that hybridizes with the target nucleic acid, and a capture probe immobilized on a support. In this method, at least one nucleic acid base in the nucleic acid molecule(s) of the detection probe and/or capture probe is substituted with a photoreactive group. The method comprises the step of irradiating, with light, a complex formed by hybridization between the target nucleic acid and the detection probe and/or capture probe to allow formation of a covalent bond(s) between the photoreactive group(s) and a nucleic acid base(s) in the target nucleic acid.

公开(公告)号：EP1729136A4

公开(公告)日：2010-07-14

申请号：EP05720981

申请日：2005-03-17

Applicant: TORAY INDUSTRIES

Inventor： TAKII YUKI ,  NAGINO KUNIHISA ,  NAKAMURA FUMIO ,  NOBUMASA HITOSHI

IPC: G01N35/02 ,  B01F13/00 ,  B01F13/02 ,  C12M1/02 ,  C12N15/09 ,  C12Q1/68 ,  G01N1/36 ,  G01N33/53 ,  G01N35/00 ,  G01N37/00

CPC classification number: B01F13/0059 ,  B01F13/0052 ,  B01F13/0054 ,  B01F13/02 ,  G01N2035/00158 ,  G01N2035/00544 ,  Y10T436/25