公开(公告)号：EP0201445A1

公开(公告)日：1986-11-12

申请号：EP86630053.6

申请日：1986-04-03

Applicant: Packard Instrument Company, Inc.

Inventor： Valenta, Robert John

IPC: G01T1/204 ,  G01T1/172

CPC classification number: G01T1/204 ,  G01T1/172

Abstract: A method and apparatus for separating background events from valid sample events in a liquid scintillation counter are disclosed. Upon the detection of a coincident event pulse the number of pulses detected during a short time interval immediately thereafter together with the energy level of the event pulse is utilized to determine the probability that the event pulse is a valid sample pulse as opposed to an invalid background pulse. The total count rate is determined by summing pulses over the appropriate energy range where each pulse is counted as one multiplied by the above probability that the pulse is a valid sample pulse.

公开(公告)号：EP0101398A1

公开(公告)日：1984-02-22

申请号：EP83630116.8

申请日：1983-07-15

Applicant: Packard Instrument Company, Inc.

Inventor： Tsai, Ten Lin Sie

IPC: C12Q1/06

CPC classification number: C12Q1/06

Abstract: A method of concentrating and measuring unicellular organism content of a sample is described. The unicellular organism containing sample is first filtered through a filter membrane impermeable to the organism to concentrate and collect the cells on the filter membrane. The membrane containing the concentrated cells is then sequentially treated (1) with a lysing agent to lyse the cells releasing the adenosine triphosphate in the cells and (2) with a luminescent reagent which reacts with the released adenosine triphosphate contained on the filter to produce light. Without significant effect by the filter membrane, the light produced on the membrane is then measured on a luminometer. Not only does this method provide an accurate way of detecting very minute quantities of cells, but it provides a relatively quick and easy method of concentrating and measuring cells in large volumes of biological or industrial liquid samples which contain low levels of organisms.

Abstract translation: 描述了浓缩和测量样品中单细胞生物体含量的方法。 首先通过对生物体不可渗透的过滤膜过​​滤含有单细胞生物体的样品，以浓缩并收集滤膜上的细胞。 然后使用裂解剂依次处理含有浓缩细胞的膜（1），以裂解释放细胞中三磷酸腺苷的细胞，和（2）用与过滤器上所含释放出的三磷酸腺嘌呤反应产生光的发光试剂 。 没有过滤膜的显着影响，然后在发光计上测量膜上产生的光。 这种方法不仅可以提供精确检测细胞数量的方法，而且可以提供一种比较快速和简便的浓缩和测量含有低水平生物体的大量生物或工业液体样品中的细胞的方法。

公开(公告)号：EP1099107B1

公开(公告)日：2009-01-07

申请号：EP99934902.0

申请日：1999-07-20

Applicant: PACKARD INSTRUMENT COMPANY

Inventor： RUSHBROOKE, John Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/64 ,  G01N21/76

CPC classification number: G01N21/6452 ,  G01N21/76 ,  G01N2021/6421 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833 ,  G01N2201/0866

Abstract: Apparatus for detecting light emitted by assay samples is provided, in which light emitted by the sample is collected for transmission to a charge coupled device camera (74) by an optical fibre bundle. The cross-sectional area of the optical fibre bundle corresponds to the area of the sample, the end of which is located close to the sample for detecting any light emitted therefrom, and selected fibres (30) of those making up the bundle are separated from the remainder and extend to a source of excitation radiation (76) and serve to convey excitation radiation (if required) directly to a corresponding plurality of points distributed over the area of the end face of the bundle and therefore over the area of the sample. The remaining fibres (32, 38) of the bundle serve to collect emitted light (whether generated by fluorescence caused by excitation or otherwise) and provide a light path to the charge coupled device camera, wherein the ends of the excitation fibres and the ends of the emitted light collecting fibres are distributed uniformly over the area of the fibre bundle presented to the reaction site.

公开(公告)号：EP1166087B1

公开(公告)日：2004-11-10

申请号：EP00901211.3

申请日：2000-01-25

Applicant: Packard Instrument Company, Inc.

Inventor： RUSHBROOKE, John Gordon, 10 Barrington House ,  HOOPER, Claire, Elizabeth

IPC: G01N21/25 ,  G02B6/06

CPC classification number: G01N21/6452 ,  G01N21/6456 ,  G02B6/4206 ,  G02B6/4298

Abstract: An optical system for imaging a multiwell sample plate onto a CCD camera, wherein light from the illuminated sample plate (26) is imaged by one or more lenses (20, 24) onto a fibre optic taper (22), bonded to the input face of the camera (28).

公开(公告)号：EP1348120A1

公开(公告)日：2003-10-01

申请号：EP01272703.8

申请日：2001-12-18

Applicant: Packard Instrument Company, Inc.

Inventor： RUSHBROOKE, John, Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6456 ,  G01N21/76 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A fibre optic epi-fluorescence imaging system in which the optical fibres are rearranged so that the system can be used for measuring luminescence samples. The system comprises at least two optical fibres (32, 46) or bundles of fibres which lead to a CCD camera (74), the fibres or bundles of fibres from all samples being arranged in two sets, a first set which are formed from a non-fluorescing material and a second set which are formed from a material which may fluoresce but enables the fibres formed therefrom to have a higher numerical aperature than those of the first set.

公开(公告)号：EP1190232A1

公开(公告)日：2002-03-27

申请号：EP00922798.4

申请日：2000-04-20

Applicant: Packard Instrument Company, Inc.

Inventor： RUSHBOOKE, John Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/25 ,  G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6458 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0833

Abstract: A method and apparatus for the measurement of radiation, especially fluorescence from samples in assays, wherein a plurality of micro-sample light emitting sites are imaged simultaneously onto a detector array by a plurality of miniature objectives, one for each sample site and focussed thereon, producing parallel beams of light arranged in parallel and spaced apart, which beams are focussed at a pinhole aperture and then reconstituted as parallel beams for incidence on the detector array.

Abstract translation: 用于测量辐射的方法和装置，特别是在测定中来自样品的荧光，其中通过多个微型物镜将多个微样品发光位点同时成像到检测器阵列上，每个样品位置一个，并且聚焦在其上， 产生平行和间隔布置的平行光束，这些光束被聚焦在针孔上，然后被重构为平行光束以入射在检测器阵列上。

公开(公告)号：EP1181099A1

公开(公告)日：2002-02-27

申请号：EP01942582.6

申请日：2001-01-11

Applicant: Packard Instrument Company, Inc.

Inventor： PAPEN, Roeland, E.

IPC: B01L3/02 ,  G01N1/10

CPC classification number: B01L3/0268 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  C40B60/14 ,  G01F1/48 ,  G01F3/00 ,  G01N35/1016 ,  G01N35/1065 ,  G01N2035/00237 ,  G01N2035/1018 ,  G01N2035/1025 ,  G01N2035/1034 ,  G01N2035/1039 ,  G01N2035/1041 ,  Y10T436/2575

Abstract: A system for aspirating and ejecting microvolume drops (26) of liquid onto porous sites of a substrate wafer includes a microdispenser (16) employing a piezoelectric transducer (60) attached to a glass capillary (62), a means for priming and aspirating transfer liquid (24) into the microdispenser (16), for controlling the pressure of the system liquid (20), and for washing the microdispenser (16) between liquid transfers, and a pressure sensor (14) to measure the system liquid pressure and produce a corresponding electrical signal. The drops are generally in the 10 to 100 micron range and the pores are generally 10 to 10,000 times smaller than the diameter of the drops deposited thereon. The resulting spots are uniform, and only slightly larger in diameter of the drops. The drops are ejected from a distance greater than the diameter of the drops, thus avoiding any contact with the dispenser that could damage the wafer. The system detects dispensing of a drop onto the reaction site.

Abstract translation: 一种用于将微量液滴（26）吸取并喷射到衬底晶片的多孔位点上的系统包括采用附着到玻璃毛细管（62）上的压电换能器（60）的微分配器（16），用于灌注和抽吸输送液体 （24）进入微分配器（16），用于控制系统液体（20）的压力，并用于在液体转移之间清洗微分配器（16），以及压力传感器（14），用于测量系统液体压力并产生 相应的电信号。 液滴通常在10到100微米的范围内，并且孔通常比在其上沉积的液滴的直径小10到10,000倍。 由此产生的斑点是均匀的，并且只有略大的液滴直径。 液滴从大于液滴直径的距离喷出，从而避免与分配器接触而损坏晶片。 该系统检测到滴落到反应部位上。

公开(公告)号：EP1068513B1

公开(公告)日：2002-01-09

申请号：EP99910524.0

申请日：1999-03-18

Applicant: PACKARD INSTRUMENT COMPANY

Inventor： RUSHBROOKE, John, Gordon ,  HOOPER, Claire, Elizabeth ,  TOMISEK, John, David

IPC: G01N21/64 ,  G01N33/542 ,  G01N33/58

CPC classification number: G01N21/6452 ,  G01N21/274 ,  G01N21/6428

Abstract: Method of performing a biomedical assay comprising the steps of: exciting the sample or samples with incident radiation of a given wavelength, thereby causing the sample to emit radiation of a different wavelength, measuring the quantity of light falling on a detector receiving the emitted radiation from the sample, thereby to produce a first measurement, illuminating the sample or samples with incident radiation in a manner which does not cause the sample to emit any significant radiation, again detecting the quantity of light falling on the detector, thereby to produce a second measurement, and correcting the first measurement with respect to the second measurement.

公开(公告)号：EP0721596B1

公开(公告)日：2000-04-19

申请号：EP95928690.7

申请日：1995-07-31

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： VALENTA, Robert, J.

IPC: G01T1/202 ,  G01T1/208

CPC classification number: G01T1/204

Abstract: A scintillation measurement system for measuring optical events produced by scintillators (19) in response to the radioactive decay of a constituent or constituents of a sample (10) to be measured comprises a sample support for positioning a sample in a sample well (11); a bismuth germanate (BGO) scintillation crystal (19), such as Bi4Ge3O12, located adjacent the sample well; a plurality of photodetectors (16, 18) located outside the bismuth germanate crystal (19) for detecting optical events occuring in the sample well (11) or in the bismuth germanate crystal (19) and converting those optical events into electrical pulses; and a pulse analyzing system (40) for receiving the electrical pulses from the photodetectors and determining whether such pulses represent alpha, beta or gamma events. This system can be used with samples containing alpha, beta or gamma emitters, or any combination thereof.

公开(公告)号：EP0645187A2

公开(公告)日：1995-03-29

申请号：EP94111503.2

申请日：1994-07-22

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： Kolb, Alfred J. ,  Manns, Roy L. ,  Neumann, Kenneth E.

IPC: B01L3/00 ,  G01T1/204 ,  G01N21/76 ,  G01T7/02

CPC classification number: B01L3/50255 ,  G01T1/204 ,  G01T7/02 ,  Y10S435/809

Abstract: A microplate assembly (10) for use in analyzing samples captured on a filter medium (16) comprises a carrier plate (12), a holding tray (14), a collimator (18), and a cover film (20). These elements are generally rectangular in shape and are sized to stack on top of one another. The holding tray (14) is positioned within the carrier plate (12), the collimator (18) and filter medium (16) are positioned within the holding tray (14) with the filter medium (16) positioned beneath the collimator (18), and the cover film (20) is sealed over the collimator (18). To prepare samples in the microplate assembly (10) for analysis, the samples are captured on the filter medium (16) and the filter medium (16) is placed in the holding tray (14). After adding scintillation cocktail or luminescent substrate to the filter medium (16), the collimator (18) is placed over the holding tray (14) with the filter medium (16) positioned between the collimator (18) and the holding tray (14) and the samples disposed in the sample wells. The carrier plate (12), the holding tray (14), the filter medium (16), and the collimator (18) are provided with complementary keyed corners (22,34,19,46) to facilitate alignment of these elements relative to one another. The wells of the collimator (18) include respective lower rims (54) protruding into the filter medium (16) to minimize crosstalk through the filter medium (16). The cover film (20) seals the microplate assembly (10) so that the samples are prepared for analysis.

Abstract translation: 用于分析在过滤介质上捕获的样品的微板组件包括载体板，保持盘，准直器和覆盖膜。 这些元件的形状通常为矩形，并且尺寸设计成彼此堆叠。 保持托盘定位在承载板内，准直器和过滤介质位于保持托盘内，过滤介质定位在准直器下方，并且覆盖膜被密封在准直器上。 为了在微板组件中制备样品用于分析，将样品捕获在过滤介质上，并将过滤介质置于保持盘中。 在过滤介质中加入闪烁混合物或发光底物之后，将准直器放置在保持盘上方，过滤介质位于准直器和保持托盘之间，样品置于样品孔中。 承载板，保持托盘，过滤介质和准直器设置有互补的键角，以便于这些元件相对于彼此对准。 准直器的阱包括突出到过滤介质中的相应的下边缘，以最小化通过过滤介质的串扰。 覆盖膜密封微板组件，使得样品准备用于分析。