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公开(公告)号：KR100744445B1
公开(公告)日：2007-08-01
申请号：KR1020060049546
申请日：2006-06-01
Applicant: (주) 차바이오텍 , 차의과학대학교 산학협력단
Abstract: A method for culturing human embryonic stem cells is provided to maintain interaction between undifferentiated human embryonic stem cells and feeder cells attached to a porous membrane, and inhibit contamination caused by enzyme treatment and feeder cells by separating only cultured stem cells from the porous membrane without enzyme treatment. The human embryonic stem cells are cultured in a medium containing the porous membrane having feeder cells in the bottom which is made of polyethylene terephthalate, polyethersulfone, polyvinylidene fluoride, cellulose, nylon, polyethylene, polypropylene, polycarbonate, polyurethane, polyacrylate, polycaprolactone and copolymers thereof and has pore diameter of 0.1-2.5 mum, wherein the feeder cell is mouse fibroblast, mouse embryonic fibroblast, human embryonic fibroblast, human bone marrow cell or adult epidermal cell.
Abstract translation: 提供培养人胚胎干细胞的方法，以保持未分化的人胚胎干细胞和附着于多孔膜的饲养细胞之间的相互作用，并且通过仅从没有酶的多孔膜分离培养的干细胞来抑制由酶处理和饲养细胞引起的污染 治疗。 将人胚胎干细胞在由聚对苯二甲酸乙二醇酯，聚醚砜，聚偏二氟乙烯，纤维素，尼龙，聚乙烯，聚丙烯，聚碳酸酯，聚氨酯，聚丙烯酸酯，聚己内酯及其共聚物制成的底部具有饲养细胞的多孔膜的培养基中培养 孔径为0.1-2.5μm，其中饲养细胞为小鼠成纤维细胞，小鼠胚胎成纤维细胞，人胚胎成纤维细胞，人骨髓细胞或成人表皮细胞。
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公开(公告)号：KR100720056B1
公开(公告)日：2007-05-18
申请号：KR1020050093989
申请日：2005-10-06
Applicant: (주) 차바이오텍
Abstract: 본 발명은 마이크로스피어 또는 마이크로비드에 부착된 성장인자에 의한 신경의 재생에 관한 것으로, 더욱 구체적으로는 카르복실기가 밖으로 나와있는 마이크로스피어 또는 마이크로비드에 양이온으로 구성된 폴리에틸렌이민으로 정전기적 결합을 시켜 양이온이 바깥쪽에 나와 있게 하여, 바깥쪽 폴리에틸렌이민에 성장인자인 섬유아세포성장인자(bFGF)를 함유한 헤파린/폴리엘라이신으로 구성된 나노입자를 정전기적 반응에 의하여 코팅을 시켜, 신경세포의 분화유도를 유발하여 신경조직재건을 위한 마이크로스피어 또는 마이크로비드는 주사형 마이크로제작 스캐폴드로 이용될 수 있다.
마이크로스피어, 마이크로비드, 성장인자, 나노입자, 신경분화, 신경재생-
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34.发明公开인간 제대혈 유래 내피 전구세포의 배양방법 및 인간 제대혈 유래 내피 전구세포를 포함하는 허혈성 질환의 예방 또는 치료용 조성물 有权用于培养从人体脐带血中衍生的内皮祖细胞的方法和组合物，用于预防或治疗包含人脐带血的内皮祖细胞的化学疾病
公开(公告)号：KR1020140091801A
公开(公告)日：2014-07-23
申请号：KR1020130003204
申请日：2013-01-11
Applicant: (주)차바이오텍
CPC classification number: C12N5/0652 , A61K35/44 , A61K35/51 , A61K38/39
Abstract: The present invention provides a method for culturing endothelial progenitor cells derived from human umbilical cord blood. According to the present invention, the endothelial progenitor cells derived from human umbilical cord blood cultured zare capable of having the cell adhesive ability and proliferation ratio similar to a cell cultured by a conventional cell culturing method. According to the present invention, collagen derived from umbilical cord and umbilical cord extract can be used as serum replacement and adjurvants for culturing a stem cell and a general animal cell. According to the present invention, a vascular endothelial (precursor) cell, and extract and collagen derived from umbilical cord, which are an autologous substance, are capable of preventing an immune rejection response generated when a cell is transplanted; and minimizing the pollution caused by a pollution source derived from an animal by excluding the use of a protein source derived from an animal. The present invention provides a pharmaceutical composition comprising endothelial progenitor cells derived from human umbilical cord blood for preventing or treating ischemic diseases.
Abstract translation: 本发明提供一种培养源自人脐带血的内皮祖细胞的方法。 根据本发明，源自人脐带血培养的能够具有与通过常规细胞培养方法培养的细胞相似的细胞粘附能力和增殖比例的内皮祖细胞。 根据本发明，可以将来自脐带和脐带提取物的胶原蛋白用作血清替代物和用于培养干细胞和一般动物细胞的辅助剂。 根据本发明，作为自体物质的来自脐带的血管内皮（前体）细胞和来源于脐带的提取物和胶原能够防止移植细胞时产生的免疫排斥反应; 并且通过排除使用源自动物的蛋白源来最小化源自动物的污染源造成的污染。 本发明提供一种包含源自人脐带血的内皮祖细胞用于预防或治疗缺血性疾病的药物组合物。
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公开(公告)号：KR101322673B1
公开(公告)日：2013-10-30
申请号：KR1020110020129
申请日：2011-03-07
CPC classification number: A61M5/329 , A61M5/3291
Abstract: 본 발명은 심근경색 또는 허혈성 심부전 질환으로 괴사된 심근조직 내에 줄기세포를 주입하기 위한 주사바늘 및 주사기에 관한 것으로, 본 발명은 주사바늘 샤프트(needle shaft)가 나선형으로 감겨진 형태인 세포주입용 주사바늘을 제공한다. 본 발명이 주사바늘 및 주사기는 심근조직에 주입 후 세포액이 심장외막 밖으로 새어나오는 것을 방지함과 동시에, 주입된 줄기세포에 혈류 순환을 재개시킴으로써 괴사된 심근조직 내에 줄기세포 생존율을 증가시킬 수 있다.
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Patent Agency Ranking
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公开(公告)号：KR1020120096793A
公开(公告)日：2012-08-31
申请号：KR1020110016103
申请日：2011-02-23
Applicant: (주)차바이오텍
CPC classification number: A61K35/28 , A61K9/0019 , A61K35/50
Abstract: PURPOSE: A composition for immunological reaction suppression including placenta originated stem cells is provided to be used for an acute or chronic Graft Versus Host Disease therapeutic agent. CONSTITUTION: A composition for immunological reaction suppression includes placenta originated stem cell as an active ingredient. The mesenchyme stem cell is originated from chorionic plate film of placenta. The mesenchyme stem cell expresses CD105, CD44, CD73 or CD90 cell surface antigens which are lobus medius indicators. The composition induces reduction of S phase and G2/M(mitotic) phase and increase of cell cycle G0/G1 phase of lymphocyte cell. The composition reduces CD69+ cell. The composition suppresses immune reaction for the transplanted organs or cells. The transplanted organs or cells are isotransplant or xenograft. The composition is used for treatment or prevention of autoimmune diseases or rejection of internal organ transplant reaction. The autoimmune disease is GVHD(graft-versus-host disease). The composition is manufactured in the injection form.
Abstract translation: 目的：提供用于免疫反应抑制的组合物，包括胎盘起始的干细胞用于急性或慢性移植物抗宿主病治疗剂。 构成：用于免疫反应抑制的组合物包括胎盘起始的干细胞作为活性成分。 间质干细胞起源于胎盘的绒毛膜片。 间质干细胞表达CD105，CD44，CD73或CD90细胞表面抗原，它们是中枢指标。 该组合物诱导淋巴细胞的S期和G2 / M（有丝分裂）期的减少和细胞周期G0 / G1期的增加。 该组合物减少CD69 +细胞。 该组合物抑制移植的器官或细胞的免疫反应。 移植的器官或细胞是异种移植或异种移植。 该组合物用于治疗或预防自身免疫性疾病或排斥内脏器官移植反应。 自身免疫性疾病是GVHD（移植物抗宿主病）。 组合物以注射形式制造。
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公开(公告)号：KR101172288B1
公开(公告)日：2012-08-09
申请号：KR1020110010057
申请日：2011-02-01
Applicant: (주)차바이오텍 , 주식회사 엘컴사이언스
Abstract: 본 발명은 천연물인 희렴으로부터 추출된 디테르펜(diterpene) 화합물을 포함하는 무혈청 배지 중에서 피부줄기세포를 배양하는 방법을 제공한다. 또한, 본 발명의 상기 배양방법으로 피부줄기세포를 배양하여 얻어진 배양물 또는 그의 분획물을 포함하는 주름개선용; 및/또는 탈모방지 또는 발모촉진용 조성물을 제공한다.
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公开(公告)号：KR1020120063377A
公开(公告)日：2012-06-15
申请号：KR1020100124510
申请日：2010-12-07
Applicant: (주)차바이오텍
IPC: C12Q1/24 , C12N5/0775
Abstract: PURPOSE: A method for isolating and culturing mesenchymal cells from embryonic stem cells is provided to cheaply isolate and culture mesenchymal cells. CONSTITUTION: A method for isolating and culturing mesenchymal cells comprises: a step of culturing embryonic stem cells or embryonic stem cell-derived embryoid body in a cell culture insert with a porous membrane; a step of isolating mesenchymal cells; and a step of culturing the mesenchymal cells. The embryonic stem cells are derived from mammal.
Abstract translation: 目的：提供从胚胎干细胞中分离和培养间充质细胞的方法，以廉价分离和培养间充质细胞。 构成：分离和培养间充质细胞的方法包括：在具有多孔膜的细胞培养插入物中培养胚胎干细胞或胚胎干细胞衍生的胚状体的步骤; 分离间充质细胞的步骤; 和培养间充质细胞的步骤。 胚胎干细胞来源于哺乳动物。
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公开(公告)号：KR1020120021699A
公开(公告)日：2012-03-09
申请号：KR1020100078202
申请日：2010-08-13
Applicant: (주)차바이오텍 , 연세대학교 산학협력단
IPC: C12N5/0735 , C12N5/0775 , C12N5/02 , C07K14/475
Abstract: PURPOSE: A method for differentiating human embryonic stem cells into mesenchymal stem cells is provided to enhance differentiation efficiency. CONSTITUTION: A method for differentiating human embryonic stem cells into mesenchymal stem cells comprises a step of culturing the human embryonic stem cells in a medium containing 80-120 ng/ml of CTGF(connective tissue growth factor) and 2-5 w/v% of FBS(fetal bovine serum) for 2-5 days with daily fresh medium.
Abstract translation: 目的：提供一种将人胚胎干细胞分化为间充质干细胞的方法，以提高分化效率。 构成：将人胚胎干细胞分化为间充质干细胞的方法包括在含有80-120ng / ml CTGF（结缔组织生长因子）和2-5w / v％的培养基中培养人胚胎干细胞的步骤， 的FBS（胎牛血清）2-5天，每日新鲜培养基。
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