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公开(公告)号：KR1020120118170A
公开(公告)日：2012-10-26
申请号：KR1020110035587
申请日：2011-04-18
Applicant: 강릉원주대학교산학협력단
IPC: A61K35/616 , A23L1/30
CPC classification number: A61K35/616 , A23L33/10
Abstract: PURPOSE: A composition for preventing or treating colon cancers which includes stichopus extracts is provided to prevent side effects by including stichopus extracts as active ingredients. CONSTITUTION: A composition for preventing or treating colon cancers which includes stichopus extracts or fractions thereof. The Stichopus is a body wall or internal organs of the Stichopus. The Stichopus fractions are chloroform fraction, hexane fraction, ethylacetate fraction or butanol fraction. The concentration of the Stichopus extracts or the fractions is 0.1-0.4 mg/mL. The Stichopus extracts or the fractions are obtained by the following steps: extracting by adding 80% methanol to stichopus and vacuumed drying thereof; dissolving the dried extract in water and partitioning thereof with chloroform; and vacuum drying the fractions. [Reference numerals] (AA) Stichopus body wall and internal organs; (BB) Freeze drying; (CC) Grinding and filteration; (DD) 80% methanol extraction; (EE) Vacuum drying; (FF) Dissolving and solvent graduation; (GG) Vacuum drying; (HH) Stichopus fraction
Abstract translation: 目的：提供一种用于预防或治疗包括粪便提取物的结肠癌的组合物，以通过包括刺槐提取物作为活性成分来防止副作用。 构成：用于预防或治疗结肠癌的组合物，其包括粪便提取物或其级分。 茎us属于茎a的体壁或内脏。 油菜馏分是氯仿馏分，己烷馏分，乙酸乙酯馏分或丁醇馏分。 刺槐提取物或级分的浓度为0.1-0.4mg / mL。 通过以下步骤获得油菜菌提取物或级分：通过向粪便中加入80％甲醇萃取并真空干燥; 将干燥的提取物溶解在水中并用氯仿分配; 并真空干燥级分。 （附图标记）（AA）柱状体壁和内脏; （BB）冷冻干燥; （CC）研磨和过滤; （DD）80％甲醇萃取; （EE）真空干燥; （FF）溶解和溶剂分级; （GG）真空干燥; （HH）Stichopus分数
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公开(公告)号：KR101098311B1
公开(公告)日：2011-12-26
申请号：KR1020090045106
申请日：2009-05-22
Applicant: 강릉원주대학교산학협력단 , 강원도립대학교산학협력단
IPC: A23L17/50 , A23L29/244
Abstract: 본원발명의대왕오징어어묵은, 곤약이첨가되어어묵의강도가높아졌고, NHCl이다량함유됨으로써비롯된대왕오징어의심한비린내가효율적으로제거되었다.
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公开(公告)号：KR101087160B1
公开(公告)日：2011-11-25
申请号：KR1020090052783
申请日：2009-06-15
Applicant: 강릉원주대학교산학협력단
IPC: A61K35/618 , A61K38/46 , A23L17/50 , A23L33/00
Abstract: 본 발명은 오징어육의 효소분해에 의한 콜레스테롤 저하용 조성물에 관한 것이다. 보다 상세하게는 전처리된 오징어육에 단백질분해효소(alcalase)를 첨가하여 오징어육을 가수분해시킨 후, 분리하여 액상, 고상 또는 분말상으로 하여 동물실험을 통하여 오징어육 효소분해물의 콜레스테롤 저하 효과를 측정하는 단계로 구성되는 혈중 콜레스테롤 저하용 조성물에 관한 것이다.
본 발명의 오징어육 효소분해물은 오징어의 내장을 분리한 오징어 육을 수세 및 탈수하는 전처리공정(1), 오징어육을 가열 및 호화하는 열처리하는 공정(2), 열처리된 오징어육을 마쇄하는 공정(3), 오징어육에 효소를 첨가하여 가수분해시키는 공정(4), 가수분해물의 분리 공정(5)으로 구성된 오징어육의 효소분해물을 이용하는 콜레스테롤 저하용 조성물이다.
본 발명은 2009년 4월 1일자로 대한민국특허청에 특허출원한 오징어육의 효소분해에 의한 기능성소재 및 그 제조방법(특허출원번호 10-2009-0028165)의 새로운 용도와 관련된 발명이다.
본 발명은 오징어육에 가열·호화공정을 채택함으로써 종래 보다 효소 가수분해공정을 개선하여 오징어육이 단백분해효소에 의하여 저분자량의 펩타이드로 잘 분해되어 혈중 콜레스테롤 저하에 효과가 있다.
오징어육, 가열·호화, 단백분해효소, 효소가수분해물, 콜레스테롤저하-
Patent Agency Ranking
公开(公告)号：KR1020100109750A
公开(公告)日：2010-10-11
申请号：KR1020090028165
申请日：2009-04-01
Applicant: 강릉원주대학교산학협력단
CPC classification number: A23L17/50 , A23L33/18 , A23V2002/00 , A23V2300/24 , A23V2300/28
Abstract: PURPOSE: A method for manufacturing a functional material by enzyme hydrolysis of cuttlefish meat is provided to efficiently improve hydrolysis process. CONSTITUTION: A method for manufacturing a functional material by enzyme hydrolysis of cuttlefish comprises: a process of washing, dehydrating and cutting cuttlefish meat; a step of heating and gelatinizing cuttlefish meat; a step of pulverizing cuttlefish meat; a step of adding enzyme and hydrolyzing; and a step of isolating hydrolysate.
Abstract translation: 目的：提供通过乌贼肉酶水解制备功能材料的方法，以有效改善水解过程。 构成：通过乌贼酶水解制备功能材料的方法包括：清洗，脱水和切割乌贼肉的过程; 加热和糊化乌贼肉的步骤; 粉碎乌贼肉的一步; 添加酶水解步骤; 和分离水解产物的步骤。
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公开(公告)号：KR1020090118577A
公开(公告)日：2009-11-18
申请号：KR1020080044450
申请日：2008-05-14
Applicant: 강릉원주대학교산학협력단 , 강원도립대학교산학협력단
Abstract: PURPOSE: A natural seasoning containing by-products obtained from processed cuttlefish, and a preparation method thereof are provided to utilize a cuttlefish internal organ as a food material. CONSTITUTION: A method for preparing a natural seasoning containing by-products obtained from processed cuttlefish comprises the following steps of: washing cuttlefish internal organs and dehydrating them; grinding the cuttlefish internal organs; mixing water, koji, salt, and ground cuttlefish internal organs at a ratio of 0.3:0.05:0.05-0.15:1; fermenting the mixture at 17~23°C for 2-5 weeks; sterilizing the mixture at 100°C for 10 minutes, and freeze-drying and grinding the mixture; and mixing the 30g mixture, 5-20g salt, 5g nucleic acid, 10g glucose, 5g red pepper powder, 3g soybean paste powder, 1g onion powder, 0.5g ginger powder, and 2g garlic powder.
Abstract translation: 目的：提供含有从加工墨鱼获得的副产物的天然调味料及其制备方法，以利用乌贼内脏为食物。 构成：制备含有由加工墨鱼获得的副产物的天然调味料的方法，包括以下步骤：清洗乌贼内脏并脱水; 研磨墨鱼内脏; 以0.3：0.05：0.05-0.15：1的比例混合水，曲，盐和地鳖鱼内脏; 在17〜23℃下将混合物发酵2-5周; 将混合物在100℃下灭菌10分钟，并冷冻干燥并研磨该混合物; 混合30g混合物，5-20g盐，5g核酸，10g葡萄糖，5g红辣椒粉，3g大豆酱粉，1g洋葱粉，0.5g姜粉和2g大蒜粉。
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公开(公告)号：KR100875267B1
公开(公告)日：2008-12-22
申请号：KR1020060033357
申请日：2006-04-12
Applicant: 강릉원주대학교산학협력단
Inventor： 김상무
Abstract: A method for preparing multi-functional hydrolyzate of Korean Gomphina veneriformis melanaegis is provided to obtain health-aid food having anti-oxidative, anti-hypertensive and anti-cancer effects by using an easily available starting material. A method for preparing multi-functional hydrolyzate of Korean Gomphina veneriformis melanaegis comprises the steps of: digesting Gomphina veneriformis melanaegis and collecting edible portions; adding the same amount of water as the edible portions and crushing the mixture; adding 1%(w/w) of protease thereto and adjusting the pH to 5.9 at 54.7 deg.C; and carrying out hydrolysis of Gomphina veneriformis melanaegis.
Abstract translation: 本发明提供了一种制备韩国桂花叶肉多功能水解液的方法，通过使用易得的起始原料，获得具有抗氧化，抗高血压和抗癌作用的保健食品。 一种韩国Gomphina venemaformis melanaegis多功能水解液的制备方法，包括以下步骤：消化Gomphina veneriformis melanaegis并收集可食部分; 加入与食用部分相同量的水并压碎混合物; 在其中加入1％（w / w）的蛋白酶，并在54.7℃下调节pH至5.9; 并进行Gomphina veneriformis melanaegis的水解。
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公开(公告)号：KR1020080086751A
公开(公告)日：2008-09-26
申请号：KR1020070028840
申请日：2007-03-23
Applicant: 강릉원주대학교산학협력단
CPC classification number: A61K8/987 , A61K8/0212 , A61K8/65 , A61K35/618 , A61Q19/00 , Y10S514/844
Abstract: Collagen extracted from outer skin of cuttlefish is provided to inhibit environmental pollution by reusing the by-products of processed cuttlefish, improve safety of use by eliminating infection risk of animal disease, improve extraction convenience, and maintain identical functionality of a mask pack containing the cuttlefish skin collagen to the conventional mask pack. A method for preparing collagen extracted from outer skin of cuttlefish comprises the steps of: washing the outer skin of cuttlefish with water, and pulverizing it in water; centrifuging the pulverized product and collecting the precipitates; adding 0.1N NaOH into the precipitates and agitating the mixture at 4 deg. C for 24 hours; and centrifuging the agitated mixture, and neutralizing and freeze-drying the precipitates. A cosmetic mask pack comprises 5.0-20.0 wt.% of the collagen extracted from outer skin of cuttlefish.
Abstract translation: 提供从乌贼外皮提取的胶原蛋白，通过重新利用加工的墨鱼的副产物来提高安全性，通过消除动物疾病的感染风险，提高提取方便性和保持含有墨鱼的面具组的功能，来抑制环境污染 皮肤胶原蛋白与常规面膜包装。 制备从乌贼外皮提取的胶原蛋白的方法包括：用水清洗乌贼外皮，并在水中粉碎; 离心粉碎产物并收集沉淀物; 向沉淀物中加入0.1N NaOH，并在4℃搅拌混合物。 C 24小时; 离心搅拌的混合物，中和并冷冻干燥沉淀物。 化妆品面膜包含5.0-20.0重量％的从墨鱼外皮提取的胶原蛋白。
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公开(公告)号：KR100757354B1
公开(公告)日：2007-09-11
申请号：KR1020060021496
申请日：2006-03-07
Applicant: 강릉원주대학교산학협력단
Inventor： 김상무
IPC: C07K14/81
Abstract: A separation method of protease inhibitor derived from Liparis tanakai eggs is provided to reduce the purification time and improve purification yield by simplifying the purification process into one step by using CNBr(cyanogens bromide)-activating sepharose 4B-papain affinity column, and inhibit heat-resistant protease causing quality lowering of fish meat. A separation method of protease inhibitor derived from Liparis tanakai eggs comprises the steps of: homogenizing Liparis tanakai eggs in sodium phosphate buffer A(pH 7.0) containing NaCl, EDTA(ethylenediamine tetraacetic acid) and 2-mercaptoethanol; centrifuging the solution to remove homogenized suspension; culturing the supernatant at 80 deg.C for 10 minutes, cooling to room temperature and centrifuging it at 10,000g for 25 minutes; fractionating the extract with ammonium sulfite; dissolving precipitated fraction in buffer A and dialyzing the solution with sodium acetate buffer B(pH 5.5) containing NaCl, NaCl, EDTA and 2-mercaptoethanol for overnight; subjecting the dialyzed fraction to the CM sepharose column using NaCl gradient; dialyzing big fractions with sodium phosphate buffer C(pH 7.5) containing NaCl, NaCl, EDTA and 2-mercaptoethanol; ultracentrifuging the fractions with a 10-kDa membrane; subjecting the concentration to the sephacryl column to obtain the crude extract; washing aliquot of the CNBr-activating sepharose 4B column and swelling it on a glass filter with HCl to prepare gel; mixing the gel with solution containing papain in coupling buffer(pH 8.3) containing NaHCO3 and NaCl and stirring it at 4 deg.C for overnight; adding the gel into a blocking agent(pH 8.0) containing glycine and stirring at 4 deg.C for overnight; washing the gel with acetate buffer(pH 4.0) containing NaCl and coupling buffer, sequentially; pouring the gel into the column having equilibrium with buffer A; passing aliquot of Liparis tanakai egg crude extract through the affinity chromatography and washing it with buffer A; and eluting trisodium phosphate buffer(pH 10) containing NaCl to the affinity chromatography in a flow rate of 0.3 mL/min.
Abstract translation: 通过使用CNBr（溴氰化氰）活化琼脂糖4B-木瓜蛋白酶亲和柱简化纯化步骤，提纯纯化时间，提纯纯化效果，提供了从Liparis tanakai卵衍生的蛋白酶抑制剂的分离方法， 抗性蛋白酶导致鱼肉质量下降。 来自Liparis tanakai卵的蛋白酶抑制剂的分离方法包括以下步骤：在含有NaCl，EDTA（乙二胺四乙酸）和2-巯基乙醇的磷酸钠缓冲液A（pH7.0）中匀化Liparis tanakai卵; 离心溶液以除去均质悬浮液; 在80℃培养上清液10分钟，冷却至室温，并以10,000g离心25分钟; 用亚硫酸铵分离提取物; 将沉淀的级分溶解在缓冲液A中并用含有NaCl，NaCl，EDTA和2-巯基乙醇的乙酸钠缓冲液B（pH5.5）透析溶液过夜; 使用NaCl梯度将透析的级分经受CM琼脂糖凝胶柱; 用含有NaCl，NaCl，EDTA和2-巯基乙醇的磷酸钠缓冲液C（pH7.5）透析大部分成分; 用10-kDa膜超速离心级分; 浓缩至sephacryl柱，得到粗提物; 洗涤CNBr活化的琼脂糖4B柱的等分试样，并用HCl在玻璃过滤器上溶胀以制备凝胶; 将凝胶与含有木瓜蛋白酶的溶液在含有NaHCO 3和NaCl的偶联缓冲液（pH8.3）中混合，并在4℃下搅拌过夜; 将凝胶加入到含有甘氨酸的封闭剂（pH8.0）中并在4℃下搅拌过夜; 用含有NaCl和偶联缓冲液的乙酸盐缓冲液（pH4.0）依次洗涤凝胶; 将凝胶倒入与缓冲液A平衡的塔中; 通过亲和层析法将Liparis tanakai蛋粗提取物通过等分试样，并用缓冲液A洗涤; 并以0.3mL / min的流速将含有NaCl的磷酸三钠缓冲液（pH10）洗脱至亲和层析。
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