누에오줌으로 부터 α-글리코시다제 억제물질의 제조방법
    31.
    发明授权
    누에오줌으로 부터 α-글리코시다제 억제물질의 제조방법 有权
    从丝光尿液制备α-淀粉酶抑制剂的方法

    公开(公告)号:KR100260612B1

    公开(公告)日:2000-07-01

    申请号:KR1019970074866

    申请日:1997-12-27

    Abstract: PURPOSE: An α-glycosidase inhibition material from silkworm urine is provided, which shows nearly same α-glycosidase inhibition effect as the existing diabetes medicine, the Acarbose, thereby contributing to increase of farm income and enhancement of national health. CONSTITUTION: A process for the preparation of α-glycosidase inhibition material from silkworm urine comprises the steps of: filtering the silkworm urine, freeze-drying to get powder, and adding methanol(alcohol-precipitation); processing anion-exchange column chromatography with the methanol-soluble part to get active part; separating into methanol-soluble part and insoluble part to remove inorganic material in silkworm urine; adding water and acetonitrile to methanol-soluble part to separate into acetonitrile layer and water layer, and concentrating the water layer and separating into A, B and C fractions(1-deoxynojirimycin, fagomine, 1,4-dideoxy-1,4-imino-D-araviniton showing excellent α-glycosidase inhibition effect).

    Abstract translation: 目的:提供蚕尿中的α-糖苷酶抑制物质,与现有糖尿病药物阿卡波糖显示几乎相同的α-糖苷酶抑制作用,从而有助于增加农民收入,提高国民健康水平。 构成:从蚕尿制备α-糖苷酶抑制物质的方法包括以下步骤:过滤蚕尿,冷冻干燥得到粉末,并加入甲醇(酒精沉淀); 用甲醇溶解部分处理阴离子交换柱层析,得到活性部分; 分离成甲醇可溶部分和不溶部分,去除蚕尿中的无机物质; 将水和乙腈加入到可溶于甲醇的部分,分离成乙腈层和水层,浓缩水层并分离成A,B和C级分(1-脱氧野尻霉素,fagomine,1,4-二脱氧-1,4-亚氨基 -D-araviniton显示出优异的α-糖苷酶抑制作用)。

    뽕나무 동정방법
    34.
    发明授权
    뽕나무 동정방법 有权
    桑树鉴定方法

    公开(公告)号:KR101566472B1

    公开(公告)日:2015-11-05

    申请号:KR1020140001589

    申请日:2014-01-07

    Abstract: 본발명은 19(서열번호 1 및 2), 28(서열번호 3 및 4), B2-3(서열번호 5 및 6), B2-26(서열번호 7 및 8), B2-28(서열번호 9 및 10), B2-42(서열번호 11 및 12), B2-48(서열번호 13 및 14) 및 B2-55(서열번호 15 및 16)의프라이머쌍을사용하는뽕나무동정방법, 키트및 프라이머조성물에관한것이다.본발명에따른뽕나무동정기술은외관상으로는용이하게구별되지않는뽕나무의유전형을보다정확하고신속하게구분할수 있기때문에, 뽕나무의중요한유전자개발, 농업적형질연구와신규품종이육성등을포함하는산업적유용성을높이는데상당히유용하다.

    뽕나무 동정방법
    35.
    发明公开
    뽕나무 동정방법 有权
    用于鉴定多毛树的方法

    公开(公告)号:KR1020150081774A

    公开(公告)日:2015-07-15

    申请号:KR1020140001589

    申请日:2014-01-07

    CPC classification number: C12Q1/6816 C12N15/11 C12Q1/686

    Abstract: 본발명은 19(서열번호 1 및 2), 28(서열번호 3 및 4), B2-3(서열번호 5 및 6), B2-26(서열번호 7 및 8), B2-28(서열번호 9 및 10), B2-42(서열번호 11 및 12), B2-48(서열번호 13 및 14) 및 B2-55(서열번호 15 및 16)로구성되는군으로부터선택되는 1종이상의프라이머쌍을사용하는뽕나무동정방법, 키트및 프라이머조성물에관한것이다. 본발명에따른뽕나무동정기술은외관상으로는용이하게구별되지않는뽕나무의유전형을보다정확하고신속하게구분할수 있기때문에, 뽕나무의중요한유전자개발, 농업적형질연구와신규품종이육성등을포함하는산업적유용성을높이는데상당히유용하다.

    Abstract translation: 本发明涉及通过使用一种或多种引物组的桑树鉴定方法,试剂盒和引物组合,所述引物组选自包含:19(SEQ ID NO:1和2)的组; 28(SEQ ID NO:3和4); B2-3(SEQ ID NO:5和6); B2-26(SEQ ID NO:7和8); B2-28(SEQ ID NO:9和10); B2-42(SEQ ID NO:11和12); B2-48(SEQ ID NO:13和14); 和B2-55(SEQ ID NO:15和16)。 本发明的桑树鉴定技术能够更准确,迅速地分离出不容易通过外观识别的桑树的基因型。 因此,本发明对于提高工业可用性,包括开发桑树重要基因,研究农业性状和新品种的育种是非常有用的。

    밀리타리스 동충하초 동정방법
    38.
    发明公开
    밀리타리스 동충하초 동정방법 有权
    识别脊髓灰质炎的方法

    公开(公告)号:KR1020140048379A

    公开(公告)日:2014-04-24

    申请号:KR1020120112845

    申请日:2012-10-11

    CPC classification number: C12Q1/686 C12Q2525/151

    Abstract: The present invention relates to an identifying method of Cordyceps militaris using a PCR method; a primer used for the method; and a Cordyceps militaris identifying kit including the primer. By using the method of the present invention, a user can accurately and rapidly identify Cordyceps militaris which is not easily identified by the outer appearance, thereby capable of being widely applied for developing various products containing Cordyceps militaris.

    Abstract translation: 本发明涉及使用PCR方法鉴定冬虫夏草的方法; 用于该方法的底漆; 和含有引物的冬虫夏草militaris鉴定试剂盒。 通过使用本发明的方法,用户可以准确而快速地鉴定出不易被外观识别的冬虫夏草,从而可广泛应用于开发含有冬虫夏草的各种产品。

    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질
    39.
    发明授权
    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질 有权
    使用丝朊蛋白和纯化蛋白质的蛋白质的质量纯化方法

    公开(公告)号:KR101346057B1

    公开(公告)日:2013-12-31

    申请号:KR1020120068585

    申请日:2012-06-26

    Abstract: The present invention relates to a mass purification method of a silkworm hemolymph-derived protein and a protein purified thereby. The mass purification method according to the present invention comprises a first step of freeze-drying silkworm to prepare a silkworm dried material; a second step of removing silk gland and obtaining the remainder hemolymph; a third step of crushing the hemolymph and adding water thereto to prepare a hemolymph lysate; a fourth step of subjecting the hemolymph lysate to centrifugation to obtain a supernatant; and a fifth step of subjecting the supernatant to column chromatography to isolate a protein of 70kDa. According to the present invention, the silkworm hemolymph is applicable for several uses, such as a cell cryopreservative, a medium supplement for cell culture, and the like. Especially, the ingredients remaining after the isolation of silkworm hemolymph are also reusable, so that the present invention contributes to an increase in the income of sericulture farmers. [Reference numerals] (AA) Silk gland removal;(BB) Grown-up silkworm;(CC) live body weight 3.13g;(DD) Centrifugation;(EE) Quick freezing;(FF) Grown-up silkworm freeze-drying 590 mg;(GG) pulverization/selection;(HH) hemolymph 380 mg;(II) Dissolution (distilled water);(JJ) 63 mg after heat treatment;(KK) 30k 70k separation;(LL) Final retrieval amount

    Abstract translation: 本发明涉及一种蚕血淋巴衍生蛋白的质量纯化方法和由此纯化的蛋白质。 根据本发明的质量净化方法包括:冷冻干燥蚕的第一步骤,制备蚕干物质; 去除丝腺并获得剩余血淋巴的第二步; 破碎血淋巴并向其中加入水以制备血淋巴溶胞产物的第三步骤; 第四步,使血淋巴溶胞产物离心得到上清液; 和将上清液进行柱色谱分离70kDa的蛋白质的第五步骤。 根据本发明,蚕血淋巴可应用于细胞冷冻保存剂,细胞培养用培养基等的多种用途。 特别是在分离蚕血淋淋后残留的成分也是可重复利用的,因此本发明有助于增加蚕食农民的收入。 (参考号)(AA)丝腺去除;(BB)成熟蚕;(CC)活体重3.13g;(DD)离心;(EE)快速冷冻;(FF)成熟蚕冷冻590 (GG)粉碎/选择;(HH)血淋巴380mg;(II)溶解(蒸馏水);(JJ)热处理后63mg;(KK)30k 70k分离;(LL)最终检索量

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