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    임파낭종병 바이러스 감염 개체 또는 내성 넙치 개체감별용 유전자 마커 및 이를 포함하는 감별 방법
    31.
    发明公开
    임파낭종병 바이러스 감염 개체 또는 내성 넙치 개체감별용 유전자 마커 및 이를 포함하는 감별 방법 无效
    用淋巴细胞病毒感染个体感染个体的方法或对于淋巴细胞病毒感染的个体感染个体

    公开(公告)号：KR1020060111144A

    公开(公告)日：2006-10-26

    申请号：KR1020050033562

    申请日：2005-04-22

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 강정하 ,  남보혜 ,  이상준 ,  김봉석 ,  김종현 ,  김경길

    IPC: C12Q1/68 ,  C12N15/12

    CPC classification number: C12Q1/6844 ,  C12N15/11 ,  C12Q1/70 ,  C12Q2521/101 ,  C12Q2600/158

    Abstract: A method for discrimination of individuals infected with lymphocystis disease virus(LCDV) or Paralichthys olivaceus individuals having resistance against lymphocystis disease virus is provided to produce of healthy individuals without virus infection, reduce lymphocystis disease occurrence rate by treating lymphocystis disease at an early stage, and selectively produce the excellent Paralichthys olivaceus individuals. A genetic marker for discrimination of fish individuals infected with lymphocystis disease virus has the nucleotide sequence of SEQ ID NO:10, wherein the genetic marker is amplified by a pair of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2, specific to NIF/NLI interacting factor gene; and the fish is Paralichthys olivaceus. The method for discrimination of fish individuals infected with lymphocystis disease virus comprises the steps of: (i) extracting the DNA sample of fish to be discriminated; (ii) amplifying the DNA sample with the primer pair of SEQ ID NO:1 and SEQ ID NO:2; and (iii) analyzing the amplification of the NIF/NLI interacting factor gene that is specific to the infection of lymphocystis disease virus.

    Abstract translation: 提供一种鉴别感染淋巴囊病病毒（LCDV）或具有抗淋巴囊病病毒的Paralichthys olivaceus个体的个体的方法，以产生没有病毒感染的健康个体，通过早期治疗淋巴囊肿病减少淋巴囊肿病发生率，以及 选择性地产生优良的Paralichthys olivaceus个体。 用于鉴定感染淋巴囊病病毒的鱼类个体的遗传标记具有SEQ ID NO：10的核苷酸序列，其中遗传标记通过具有SEQ ID NO：1和SEQ ID NO：1的核苷酸序列的一对引物扩增。 2，特异性NIF / NLI相互作用因子基因; 鱼是Paralichthys olivaceus。 用于鉴别感染淋巴囊病病毒的鱼类个体的方法包括以下步骤：（i）提取待鉴别的鱼的DNA样品; （ii）用SEQ ID NO：1和SEQ ID NO：2的引物对扩增DNA样品; 和（iii）分析淋巴囊性疾病病毒感染特异的NIF / NLI相互作用因子基因的扩增。

    넙치 암수 구분 단일염기다형성 마커
    38.
    发明授权
    넙치 암수 구분 단일염기다형성 마커 有权
    用于在OLIVE FLOUNDER中识别性别的SNP标记

    公开(公告)号：KR101499689B1

    公开(公告)日：2015-03-09

    申请号：KR1020140124525

    申请日：2014-09-18

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 김우진 ,  안철민 ,  공희정 ,  김현철 ,  김영옥 ,  남보혜 ,  김성연

    IPC: C12Q1/68

    CPC classification number: C12Q1/6844 ,  C12Q1/6813

    Abstract: 본 발명은 넙치 암수 유전체를 비교 분석하여 넙치 종묘의 암수를 유전적으로 판별할 수 있는 단일염기다형성(SNP) 마커를 제공한다.

    Abstract translation: 本发明的目的是在性别歧视之前提供用于区分橄榄比目鱼的性别的标记。 该方法中提供的是单核苷酸多态性（SNP）标记，通过比较和分析男性和女性橄榄苜蓿的基因组，可以一般地区分橄榄比目鱼的性别。 提供了一种DNA多核苷酸或补偿其的多核苷酸，用于区分选自包含各种多核苷酸的组的橄榄比目鱼的性别。

    폴딩되는 큰 활성 단백질의 수용성 발현
    39.
    发明授权
    폴딩되는 큰 활성 단백질의 수용성 발현 有权
    大体积折叠的活性蛋白的可溶性表达

    公开(公告)号：KR101184011B1

    公开(公告)日：2012-09-27

    申请号：KR1020100043855

    申请日：2010-05-11

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 이상준 ,  김영옥 ,  남보혜 ,  공희정 ,  김경길

    IPC: C12N15/63 ,  C12N15/11

    CPC classification number: C12N15/625 ,  C07K2319/02 ,  C07K2319/50 ,  C12N15/70 ,  C12P21/06

    Abstract: The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.

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