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公开(公告)号：KR101540696B1
公开(公告)日：2015-08-03
申请号：KR1020140179759
申请日：2014-12-12
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/68
CPC classification number: C12Q1/6813 , C12Q1/6888 , C12Q2525/151 , C12Q2537/143 , C12Q2600/124 , C12Q2600/16
Abstract: 본발명은바이러스에내성을갖는넙치육종방법에관한것으로, 본발명의바이러스에내성을갖는넙치육종방법으로생산된넙치는 L3, L4, L5, L6, L8, L9, L10 및 L11로구성된 8개의마이크로새틀라이트(microsatellite) 마커에특이적인프라이머를이용하여분석된 8개유전좌위의대립유전형의크기가 L3는 217~249bp, L4는 288~326bp, L5는 87~101bp, L6는 114~140bp, L8은 234~294bp, L9는 80~101bp, L10은 152~188bp 및 L11은 206~254bp인염기쌍(base pairs)을갖고, 바이러스성출혈성패혈증바이러스(viral hemorrhagic septicemia virus, VHSV)에내성이있으며, 빠른성장속도를나타냄으로써, 질병에강한넙치품종을개발하여양식업에유용하게사용될수 있다.
Abstract translation: 本发明涉及一种用于育种耐病性橄榄苜蓿的方法。 通过该方法培养的抗病毒橄榄苜蓿包括碱基对，其中通过使用对由L3，L4，L5，L6，L8，L9，L10和L11组成的8个微卫星标记特异的引物分析的8个基因座位点处的等位基因为217 -249bp，288-326bp，87-101bp，114-140bp，234-294bp，80-101bp，152-188bp和206-254bp的大小。 此外，耐病毒的橄榄苜蓿对病毒性出血性败血病病毒（VHSV）具有抗性，并且显示出快速的生长速率。 因此，该方法能够通过开发一种抗病能力的橄榄苜蓿种，有效地应用于水产养殖业。
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公开(公告)号：KR1020030014814A
公开(公告)日：2003-02-20
申请号：KR1020010048692
申请日：2001-08-13
Applicant: 대한민국(관리부서:국립수산과학원)
Inventor： 최광식 , 강상균 , 불가코브알렉산더에이 , 김윤 , 김성연
IPC: G01N33/53
Abstract: PURPOSE: A quantitative measurement method of reproductive output of Crassostrea gigas using immune reaction is provided, thereby easily and correctly determining the reproductive output of marine mollusk. CONSTITUTION: The quantitative measurement method of reproductive output of Crassostrea gigas using immune reaction comprises the steps of: collecting eggs of Crassostrea gigas to obtain an antigen; subjecting the eggs of Crassostrea gigas to ultrasonic wave and centrifuging the broken eggs to obtain anti-serum using the antigen; removing antibody exhibiting cross-reaction with the body tissue of Crassostrea gigas using immune adsorption method to prepare an antibody specific to Crassostrea gigas eggs; reaction of the Crassostrea gigas eggs antibody with total extract of Crassostrea gigas; and measuring the degree of the reaction, wherein the immune adsorption method uses glutaraldehyde; and the quantification of the Crassostrea gigas eggs protein uses ELISA.
Abstract translation: 目的：提供使用免疫反应的Crassostrea gigas生殖产量的定量测量方法，从而容易且正确地确定海洋软体动物的繁殖产量。 构成：使用免疫反应的Crassostrea gigas生殖产量的定量测定方法包括以下步骤：收集Crassostrea gigas的卵以获得抗原; 将Crassostrea gigas的卵子超声波进行超声波离心并离心分离的卵，使用抗原获得抗血清; 使用免疫吸附法去除与Crassostrea gigas的身体组织交叉反应的抗体，以制备对Crassostrea gigas蛋鸡特异的抗体; Crassostrea gigas egg抗体与Crassostrea gigas的总提取物的反应; 并测量反应程度，其中免疫吸附法使用戊二醛; 并且Crassostrea gigas egg蛋白的定量使用ELISA。
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公开(公告)号：KR101499689B1
公开(公告)日：2015-03-09
申请号：KR1020140124525
申请日：2014-09-18
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , C12Q1/6813
Abstract: 본 발명은 넙치 암수 유전체를 비교 분석하여 넙치 종묘의 암수를 유전적으로 판별할 수 있는 단일염기다형성(SNP) 마커를 제공한다.
Abstract translation: 本发明的目的是在性别歧视之前提供用于区分橄榄比目鱼的性别的标记。 该方法中提供的是单核苷酸多态性（SNP）标记，通过比较和分析男性和女性橄榄苜蓿的基因组，可以一般地区分橄榄比目鱼的性别。 提供了一种DNA多核苷酸或补偿其的多核苷酸，用于区分选自包含各种多核苷酸的组的橄榄比目鱼的性别。
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公开(公告)号：KR1020100117497A
公开(公告)日：2010-11-03
申请号：KR1020090131380
申请日：2009-12-28
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: A01N59/08 , A01N1/0221 , A01N1/0226 , A01N25/02 , A01N25/22
Abstract: PURPOSE: An artificial seminal plasma for preserving grouper is provided to store sperm for a long time and to simplify preservation method. CONSTITUTION: An artificial seminal plasma for grouper contains 0.01 w/v weight% of potassium chloride(KCl), 0.9w/v structure contains 0.01w/v% of potassium chloride, 0.9 2/v% of sodium chloride, 0.006 wt% of calcium chloride, 0.008 w/v% magnesium chloride, 0.05 w/v% of sodium bicarbonate, and 0.002 w/v% of arbumin.
Abstract translation: 目的：提供用于保存石斑鱼的人造精浆等待长时间储存精子，简化保存方法。 构成：石斑鱼的人造精液含有0.01w / v氯化钾（KCl）重量％，0.9w / v结构含有0.01w / v％氯化钾，0.9 2 / v％氯化钠，0.006wt％ 氯化钙，0.008w / v％氯化镁，0.05w / v％碳酸氢钠和0.002w / v％的白蛋白。
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Patent Agency Ranking
公开(公告)号：KR100436027B1
公开(公告)日：2004-06-14
申请号：KR1020010048692
申请日：2001-08-13
Applicant: 대한민국(관리부서:국립수산과학원)
Inventor： 최광식 , 강상균 , 불가코브알렉산더에이 , 김윤 , 김성연
IPC: G01N33/53
Abstract: PURPOSE: A quantitative measurement method of reproductive output of Crassostrea gigas using immune reaction is provided, thereby easily and correctly determining the reproductive output of marine mollusk. CONSTITUTION: The quantitative measurement method of reproductive output of Crassostrea gigas using immune reaction comprises the steps of: collecting eggs of Crassostrea gigas to obtain an antigen; subjecting the eggs of Crassostrea gigas to ultrasonic wave and centrifuging the broken eggs to obtain anti-serum using the antigen; removing antibody exhibiting cross-reaction with the body tissue of Crassostrea gigas using immune adsorption method to prepare an antibody specific to Crassostrea gigas eggs; reaction of the Crassostrea gigas eggs antibody with total extract of Crassostrea gigas; and measuring the degree of the reaction, wherein the immune adsorption method uses glutaraldehyde; and the quantification of the Crassostrea gigas eggs protein uses ELISA.
Abstract translation: 目的：提供一种利用免疫反应定量测定牡蛎繁殖量的方法，从而方便，准确地确定海洋软体动物的繁殖量。 本发明公开了一种利用免疫反应定量测定牡蛎繁殖量的方法，包括以下步骤：采集太平洋牡蛎蛋以获得抗原; 用超声波对龙骨牡蛎的卵进行超声波处理，并使用该抗原离心破碎的卵以获得抗血清; 使用免疫吸附法去除表现出与太平洋牡蛎（Crassostrea gigas）的身体组织交叉反应的抗体以制备特异于巨牡蛎蛋的抗体; Crassostrea gigas卵抗体与Crassostrea gigas总提取物的反应; 并测量反应程度，其中免疫吸附法使用戊二醛; 并且对太平洋牡蛎蛋白质的定量使用ELISA。
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公开(公告)号：KR101272500B1
公开(公告)日：2013-06-11
申请号：KR1020120130630
申请日：2012-11-19
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A01K63/00
CPC classification number: A01K63/003 , A01K63/006 , A01K63/04
Abstract: PURPOSE: A partitioned water tub capable of rotating, attaching and detaching is provided to prevent stress caused by accommodating different kinds of fishes, to extend the life time of fishes, to improve space use of the tub, and to secure convenience for managing fishes according to their sizes. CONSTITUTION: An outer pipe(40) is also formed inside the tub with a drain hole, and an inner pipe(60) connected to the outside of the main body is formed inside the outer pipe. The partition of the tub has a form selected from one between: partitioning the inside by contacting the partition frame and the partition fence in a straight line, and partitioning the inside by contacting the partition wall and the partition frame in the state that their sides are partially out of joint.
Abstract translation: 目的：提供一种能够旋转，分离和分离的分隔式水桶，以防止因各种鱼类而适应的压力，延长鱼类使用寿命，提高鱼缸的使用空间，确保管理鱼类的便利性 到他们的大小。 构成：在桶内还形成有排水孔的外管（40），在外管内部形成有与主体外侧连接的内管（60）。 桶的隔板具有从以下之一形成的形式：以直线分隔与分隔框架和隔板接触的方式分隔内侧，并且以分隔壁与分隔框架的侧面接触来分隔内侧 部分脱节。
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公开(公告)号：KR101109410B1
公开(公告)日：2012-06-27
申请号：KR1020090131380
申请日：2009-12-28
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: 본 발명은 자바리 정자의 냉동보존용 인공정장에 관한 것으로서, 더욱 상세하게는 정자 냉동보존 효과를 극대화하기 위해 자바리 정자의 냉동보존에 적합한 동해방지제를 제안하고, 자바리 정액의 특성을 파악하여 종 특이적인 희석액(인공정장)을 제안하는 것이다. 자바리 정자의 냉동보존용 인공정장과 적정 동해방지제는 정자를 장기간 보존할 수 있도록 할 뿐만 아니라, 자바리처럼 암컷에서 수컷으로 성전환을 하여 성숙한 수컷을 얻기 어렵거나 암수의 성비가 맞지 않아 인공수정이 어려운 경우 매우 유용함으로써 향후 자바리의 종 보존방법을 간소화시킬 수 있는 방안을 제시할 것이다.
본 발명은 염화칼륨(KCl) 0.01 w/v%와, 염화나트륨(NaCl) 0.9 w/v%와, 염화칼슘(CaCl
2 ) 0.006 w/v%와, 염화마그네슘(MgCl
2 ) 0.008 w/v%와, 중탄산나트륨(NaHCO
3 ) 0.05 w/v%와, 알부민 0.002 w/v%로 이루어지는 것에 특징이 있다.
자바리, 정자, 냉동보존, 동해방지제, 희석액, 인공정장-
公开(公告)号：KR100515011B1
公开(公告)日：2005-09-13
申请号：KR1020030054947
申请日：2003-08-08
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A61D19/02
Abstract: 본 발명은 범가자미 정자 냉장보존을 위한 인공정장에 관한 것으로서, 범가자미의 정액 중 정장의 이온조성 K
+ , Na
+ , Cl
- , Ca
2+ , Mg
2+ 및 이들의 함량과 동일하도록 KCl, NaCl, CaCl
2 , MgCl
2 , NaH
2 PO
4 , NaHCO
3 및 Glucose를 포함하여 구성되는 물질을 혼합하여 얻어지는 인공정장을 제공한다. 본 발명에 따르면, 범가자미로부터 추출된 정자를 냉장상태로 3주 이상 안전하게 보관할 수 있다.-
公开(公告)号：KR1020050017634A
公开(公告)日：2005-02-22
申请号：KR1020030054947
申请日：2003-08-08
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A61D19/02
CPC classification number: A61D19/022
Abstract: PURPOSE: Artificial seminal plasma for cool preservation of spotted flounder sperm is provided which is capable of preserving the spotted flounder sperm for more than three weeks safely in a refrigerated state, thereby greatly contributing to development of the culturing industry. CONSTITUTION: The artificial seminal plasma comprises: KCl of 2.6-3.5%, NaCl of 76.0-82.8% and MgCl_2 of 2.1-8.4%; NaH_2PO_4 of 0.3-0.5%; NaHCO_3 of 4.6-5.2%; Glucose of 0.9-2.1%; and (N-£2-Hydroxyethyl|piperazine-N'-£2-ethanesulfonic acid|)(HEPES) of 6.5-7.4%.
Abstract translation: 目的：提供人造精液等离子体，用于冷藏保存斑点比目鱼精子，能够在冷藏状态下安全保存斑点的比目鱼精子超过三周，从而大大有助于培养产业的发展。 构成：人造精浆含有：KCl为2.6-3.5％，NaCl为76.0-82.8％，MgCl_2为2.1-8.4％; NaH_2PO_4为0.3-0.5％; NaHCO 3为4.6-5.2％; 葡萄糖0.9-2.1％; 和（N-乙基-2-乙氧基乙基|哌嗪-N'-乙烷磺酸|）（HEPES）为6.5-7.4％。
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