Abstract:
A method for evaluating sheath blight resistance is provided to obtain an objective evaluation result conveniently regarding the sheath blight resistance, raise the inoculation efficiency of pathogenic bacteria of the sheath blight and improve the resistance evaluation method, thereby contributing to breed a species with resistance to the sheath blight. A method for evaluating sheath blight resistance comprises the steps of: (a) after inoculating a liquid culture mycelia of Rhizoctonia solani into rice, sealing the inoculation portion tightly using a shielding film such as aluminum foil for maintaining moisture required for vitality of the mycelia; and (b) after cultivating the mycelia inoculated rice until disease is progressed into branches and leaves, evaluating the resistance degree, wherein the resistance degree evaluation is performed by evaluating the attack degree of all leaves sheaths corresponding to the inoculated stem with 0 to 9 steps and calculating a virus infection rate of the inoculated stem in accordance with a following calculating formula of the virus infection rate being equal to (9n9+7n7+5n5+3n3+1n1+0n0)/9N Î 100(wherein each of the n0 to n9 is a number of leaves sheaths corresponding to each of the attack degree evaluated at the mycelia inoculated stem, and the N is the total leaves sheaths of the mycelia inoculated stem).
Abstract:
PURPOSE: Provided is a novel microorganism which solubilizes fixed phosphates in soil of volcanic lime ash, thereby utilizing phosphates fixed in soil of volcanic lime ash in a tangerine orchard. CONSTITUTION: A novel microorganism strain, Bacillus spharicus PSB-13(KCTC 18005P) is excellent in phosphate solubilization and easily adapts itself to the condition of soil of volcanic lime ash. It shows viability at pH 4-7 and 4-70 deg.C and has an organic acid productability to produce citric acid and oxalic acid. It is also easily mixed with organics and used.