-
公开(公告)号：KR101444196B1
公开(公告)日：2014-09-26
申请号：KR1020120129111
申请日：2012-11-14
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: 본 발명은 신규한 미생물 바실러스 코아귤란스(
Bacillus
coagulans ) KM-1, 이를 이용한 발효대두박 및 양어용 사료에 관한 것으로, 보다 상세하게는 알파-갈락토시다제 효소활성을 가지며 난소화성 탄수화물 분해활성이 우수한 신규한 미생물 바실러스 코아귤란스 KM-1 균주를 이용하여 대두박을 고온발효시킴으로써 기존의 대두박보다 소화율이 향상된 양어용 사료를 제조하는 방법에 관한 것이다.-
公开(公告)号：KR101374194B1
公开(公告)日：2014-03-13
申请号：KR1020130055554
申请日：2013-05-16
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C07K14/435 , C12N15/12 , C12N15/63 , A61K38/17
Abstract: The present invention relates to a peptide representing serine protease inhibition activity and firstly separated from Fenneropenaeus chinensis, and proved anti-trypsin activity and anticoagulant activity. According to an embodiment of the present invention, a novel serine protease inhibitor is separated from Fenneropenaeus chinensis and identified, and the present invention proves effects of anti-trypsin activity and anticoagulant activity thereof, thereby efficiently applying the same as an active ingredient of anticoagulants. [Reference numerals] (AA) Anticoagulant activity(Prolongation of APTT(s))
Abstract translation: 本发明涉及一种代表丝氨酸蛋白酶抑制活性的肽，首先从中华ensis ensis分离，证明了抗胰蛋白酶活性和抗凝血活性。 根据本发明的一个实施方案，将新的丝氨酸蛋白酶抑制剂从中华龙胆中分离出来，鉴定出来，本发明证明了抗胰蛋白酶活性和抗凝血活性的作用，从而有效地将其作为抗凝剂的活性成分施用。 （参考号）（AA）抗凝血活性（延长APTT）
-
公开(公告)号：KR1020130003572A
公开(公告)日：2013-01-09
申请号：KR1020110065002
申请日：2011-06-30
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: PURPOSE: A novel strain which decomposes marine-derived polybutylene succinate(PBS) and a method for decomposing PBS using the same are provided to effectively decompose waste fishing gear made of a PBS material. CONSTITUTION: A Staphylococcus sp. PBS-1(KACC91577) is able to decompose polybutylene succinate(PBS). A Paenibacillus sp. PBS-2(KACC91578) is able to decompose PBS. A composition for decomposing PBS contains the Staphylococcus sp. PBS-1 or Paenibacillus sp. PBS-2. A method for decomposing PBS comprises a step of treating the Staphylococcus sp. PBS-1 or Paenibacillus sp. PBS-2 to PBS. Waste fishing gear contains PBS.
Abstract translation: 目的：提供一种分解海洋衍生的聚丁二酸丁二醇酯（PBS）的新型菌株以及使用其分解PBS的方法，以有效分解由PBS材料制成的废物渔具。 构成：葡萄球菌属 PBS-1（KACC91577）能够分解聚丁二酸丁二醇酯（PBS）。 一种Paenibacillus sp。 PBS-2（KACC91578）能够分解PBS。 用于分解PBS的组合物含有葡萄球菌属。 PBS-1或Paenibacillus sp。 PBS-2。 分解PBS的方法包括处理葡萄球菌属的步骤。 PBS-1或Paenibacillus sp。 PBS-2至PBS。 废物渔具包含PBS。
-
公开(公告)号：KR1020110124471A
公开(公告)日：2011-11-17
申请号：KR1020100043855
申请日：2010-05-11
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12N15/625 , C07K2319/02 , C07K2319/50 , C12N15/70 , C12P21/06
Abstract: PURPOSE: A method for improving soluble expression of a target protein is provided to prevent insoluble precipitation of a recombinant protein and to improve recombinant protein secretion. CONSTITUTION: An expression vector for improving soluble expression and secretion of an active protein in which a membrane potential domain is folded contains: a promoter; and a gene construct which is operatively linked to the promoter and contains a polynucleotide encoding a leader polypeptide. A method for improving soluble expression and secretion of the active protein comprises: a step of designing a polynucleotide encoding a polypeptide with pI value of N-terminal to 2.00-6.00 and hydrophilic value to 1.00-2.00; a step of preparing a gene construct comprising the polynucleotide and a polynucleotide encoding the active protein; a step of operatively inserting the gene construct into an expression vector to prepare a recombinant expression vector; a step of transducing the recombinant expression vector to a host cell to form a transformant; and a step of culturing the transformant.
Abstract translation: 目的：提供一种改善靶蛋白可溶性表达的方法，以防止重组蛋白的不溶性沉淀，并改善重组蛋白的分泌。 构成：用于改善其中折叠膜电位域的活性蛋白质的可溶性表达和分泌的表达载体包含：启动子; 和与启动子有效连接并含有编码前导多肽的多核苷酸的基因构建体。 一种用于改善活性蛋白质的可溶性表达和分泌的方法包括：将编码pI值为N-末端的多肽的多核苷酸设计为2.00-6.00，亲水性值设为1.00-2.00的步骤; 制备包含所述多核苷酸的基因构建体和编码所述活性蛋白质的多核苷酸的步骤; 将基因构建体可操作地插入表达载体以制备重组表达载体的步骤; 将重组表达载体转导到宿主细胞以形成转化体的步骤; 以及培养转化体的步骤。
-
公开(公告)号：KR101047322B1
公开(公告)日：2011-07-13
申请号：KR1020090056632
申请日：2009-06-24
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: 본 발명의 뱀장어 유전자 감별법은 뱀장어 종간의 다형성 및 특정 서열의 반복횟수를 PCR을 이용하여 정확하게 구분할 수 있으므로 미량의 뱀장어 조직을 이용하여 간편하고 신속한 방법으로 국내산 실뱀장어와 외국산 실뱀장어를 정확하게 판별할 수 있다.
뱀장어, 유전자 감별법, RAPD, SCAR, PCR, 다형성-
Patent Agency Ranking
公开(公告)号：KR1020110018646A
公开(公告)日：2011-02-24
申请号：KR1020090076221
申请日：2009-08-18
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: PURPOSE: A novel gene encoding Paralichthys olivaceus-derived antibacterial peptide, bea-defensin is provided to ensure pivotal immune response function and to be used in developing natural antibiotics. CONSTITUTION: A Paralichthys olivaceus-derived antibacterial polypeptide comprises: a polypeptide encoded by cDNA having one of nucleic acid sequence among sequence numbers 1-5; a polypeptide encoded by gDNA having one of nucleic acid among sequence numbers 6-10; or a polypeptide having one of amino acid sequence among sequence numbers 11-15. The antibacterial polypeptide is anionic. Antibiotics or feed additives contain the antibacterial polypeptide, mutant thereof, or active fragment thereof as an active ingredient.
Abstract translation: 目的：提供一种编码Paralichthys olivaceus来源的抗菌肽，bea-defensin的新基因，以确保关键的免疫应答功能，并用于开发天然抗生素。 构成：来自Paralichthys olivaceus的抗菌多肽包含：由序列号1-5之一的核酸序列之一的由cDNA编码的多肽; 由序列号6-10中具有核酸之一的gDNA编码的多肽; 或具有序列号11-15之一的氨基酸序列的多肽。 抗菌多肽是阴离子的。 抗生素或饲料添加剂含有作为活性成分的抗菌多肽，其突变体或其活性片段。
-
公开(公告)号：KR1020040045267A
公开(公告)日：2004-06-01
申请号：KR1020030018573
申请日：2003-03-25
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12N9/14
CPC classification number: C12N9/16 , A23K20/189 , C12N15/70
Abstract: PURPOSE: Phytase derived from Citrobacter braakii is provided, which phytase can be used in domestic animal feed because it has improved enzyme activity, cannot be decomposed in the stomach and intestines because it has tolerance to hydrolase. CONSTITUTION: The phytase derived from Citrobacter braakii has the following properties: (a) 47 kDa of molecular weight on SDS-PAGE, (b) pH 3.5 to 4.5 of optimal pH, (c) 45 to 55 deg. C of optimal temperature, (d) substrate specificity wherein the substrates are phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) 3.5 to 0.5 mM of Michaelis constant when the substrate is phytate, and (f) high tolerance to a protein decomposing enzyme selected from pepsin, trypsin, papain, elastase and pancreatin, wherein the phytase comprises the amino acid sequence set forth in SEQ ID NO: 2 in the N-terminal. A gene encoding the phytase derived from Citrobacter braakii has the nucleotide sequence set forth in SEQ ID NO: 6. A phytase producing Citrobacter braakii YH-15(KCCM 10427) is provided.
Abstract translation: 目的：提供从柠檬酸杆菌衍生的植酸酶，其中植酸酶可用于家畜饲料，因为它具有改善的酶活性，不能在胃和肠中分解，因为它具有对水解酶的耐受性。 构成：来自柠檬酸杆菌的植酸酶具有以下性质：（a）SDS-PAGE上分子量为47kDa，（b）最佳pH为3.5至4.5，（c）为45至55℃。 （d）底物特异性，其中底物是植酸盐，对硝基苯基磷酸盐，焦磷酸四钠，ATP或ADP，（e）当底物植酸时，3.5至0.5mM的米氏常数，和（f）高耐受性 涉及选自胃蛋白酶，胰蛋白酶，木瓜蛋白酶，弹性蛋白酶和胰酶的蛋白质分解酶，其中所述植酸酶包含N-末端中SEQ ID NO：2所示的氨基酸序列。 编码来自枸橼酸杆菌的植酸酶的基因具有SEQ ID NO：6所示的核苷酸序列。提供了产生柠檬酸杆菌YH-15（KCCM 10427）的植酸酶。
-
公开(公告)号：KR101583541B1
公开(公告)日：2016-01-12
申请号：KR1020130166125
申请日：2013-12-27
Applicant: 대한민국(관리부서:국립수산과학원) , 주식회사 엘지생활건강
Abstract: 본발명은기능성화장품을제조에이용될수 있는, 기탁번호 KCCM11422P로기탁된, 해양유래신규테나시바큘럼디스칼라 RSS4(RSS4) 균주를제공한다. 상기균주는주름개선, 피부항염, 피부미백, 피부항산화및 피부미백의효과를발휘하는발효성분의제조에유용하게사용될수 있으며, 펄감을나타낼수 있는기능성화장품및 항염증약학적조성물등의안료원료로유용하게사용될수 있다.
-
公开(公告)号：KR101514452B1
公开(公告)日：2015-04-27
申请号：KR1020130031542
申请日：2013-03-25
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: 본 발명은 리파아제를 생산하며, 기탁번호 KCCM11366P로 기탁된, 신규 포토박테리움 MA1-3 균주(
Photobacterium MA1-3), 상기 균주로부터 생산되는 리파아제 및 상기 리파아제를 이용하는 바이오디젤 제조방법을 제공한다.Abstract translation: 本发明涉及产生脂肪酶并以保藏号KCCM11366P保藏的新型光合细菌MA1-3菌株（以下称为“菌株”），
-
公开(公告)号：KR1020130136381A
公开(公告)日：2013-12-12
申请号：KR1020130052956
申请日：2013-05-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12N15/70
Abstract: The present invention relates to an expression vector for controlling total expression of Active foreign proteins comprising 1) a promoter, 2) a restriction enzyme recognition unit for inserting polynucleotide encoding foreign proteins including translation termination codon, and 3) a gene construct in which trinucleotide repeated body of hexanucleotide are linked in a sequence manner.
Abstract translation: 本发明涉及用于控制活性外源蛋白质的总表达的表达载体，其包括1）启动子，2）用于插入编码包含翻译终止密码子的外源蛋白的多核苷酸的限制酶识别单元，和3）三核苷酸重复的基因构建体 六核苷酸体以序列方式连接。
-
-
-
-
-
-
-
-
-
Search Results
65
records
(took 0.029 seconds)
