公开(公告)号：KR1020080114192A

公开(公告)日：2008-12-31

申请号：KR1020070063532

申请日：2007-06-27

Applicant: 주식회사 중앙백신연구소 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 주한수 ,  윤인중 ,  이경원 ,  강성호 ,  최환원 ,  유성식 ,  현방훈 ,  송재영 ,  조인수 ,  최은진 ,  윤소라 ,  김인중 ,  표현미 ,  이창희

IPC: A61K39/145 ,  A61K39/12 ,  A61P11/00

CPC classification number: A61K39/12 ,  A61K2039/552 ,  Y10S424/815 ,  Y10S424/825

Abstract: A method of manufacturing a vaccine composition containing antigen extracted from pig tissue infected with a complex respiratory disease is provided to prevent and treat a post weaning multisystemic wasting syndrome(PMWS) and a pig respiratory organ complex syndrome(PRDC). A method of manufacture the vaccine composition comprises steps of: i) removing pig tissue infected with a complex respiratory diseases; ii) extracting antigen by adding a solution comprising a glycine buffer solution and triton X-100 of 0.5% to the tissue; and iii) being inactivated by a formalin.

Abstract translation: 提供含有由感染复合呼吸系统疾病的猪组织提取的抗原的疫苗组合物的制造方法，以预防和治疗断乳多系统消耗综合征（PMWS）和猪呼吸器官综合征综合征（PRDC）。 制备疫苗组合物的方法包括以下步骤：i）去除感染复杂呼吸系统疾病的猪组织; ii）通过向组织中加入包含甘氨酸缓冲液和0.5％的triton X-100的溶液来提取抗原; 和iii）被福尔马林灭活。

公开(公告)号：KR100543691B1

公开(公告)日：2006-01-20

申请号：KR1020020079085

申请日：2002-12-12

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 송재영 ,  최은진 ,  박종현 ,  안동준 ,  차상호 ,  권준헌

IPC: G01N33/543

Abstract: 본 발명은 항원-항체 반응을 이용하여 신속하게 돼지콜레라바이러스에 대한 항체의 존재유무를 검출할 수 있도록 고안된 검출 키트 및 이를 이용한 돼지콜레라바이러스 항체의 검출 방법에 관한 것이다.
돼지콜레라, 면역크로마토그라피, 골드 콘쥬게이트, 유전자 재조합

公开(公告)号：KR100430936B1

公开(公告)日：2004-05-14

申请号：KR1020010083879

申请日：2001-12-24

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 강승원 ,  최은진 ,  정우석

IPC: A61K39/002

Abstract: PURPOSE: An attenuated vaccine prepared by isolating a Theileria sergenti Sungwhan stock from Haemaphysalis longicornis and then attenuating it is provided. The vaccine has recognizable specific protection property and thus can be effectively used in the prevention of bovine theileriosis. CONSTITUTION: An attenuated vaccine is prepared by the steps of: recovering Haemaphysalis longicornis imagoes from bovines infected with theileriosis; infecting a rabbit with the Haemaphysalis longicornis imagoes for 6 to 7 days; and aseptically collecting salivary gland from the collected Haemaphysalis longicornis imagoes. The vaccine is formed into an injectable formulation.

Abstract translation: 目的：提供一种减毒疫苗，它是通过从长角血蜱Haemaphysalis longicornis中分离泰勒虫（Theileria sergenti Sungwhan），然后减毒来制备的。 该疫苗具有可识别的特异性保护特性，因此可有效用于预防牛泰勒虫病。 组成：减毒疫苗是通过以下步骤制备的：从感染泰勒氏菌病的牛中回收长角血蜱（Haemaphysalis longicornis）成虫; 用长角血蜱成虫感染兔子6至7天; 并从收集的Haemaphysalis longicornis成虫中无菌收集唾液腺。 疫苗形成可注射制剂。

公开(公告)号：KR101777252B1

公开(公告)日：2017-09-12

申请号：KR1020160086210

申请日：2016-07-07

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  베터리너리 메디컬 리서치 앤 디벨롭먼트

Inventor： 차상호 ,  우인옥 ,  유지은 ,  최은진 ,  송재영 ,  조인수 ,  청원조셉청 ,  아만다린그림

IPC: G01N33/50

CPC classification number: G01N33/5017 ,  G01N33/505 ,  G01N33/5055 ,  G01N2333/005 ,  G01N2333/195

Abstract: 본발명은 T 세포의병원체감염세포증식억제능평가방법및 세포억제능에관여하는병원체특이적 T 세포의정보제공방법에관한것이다. 본발명의 T 세포의병원체감염세포증식억제능평가방법은직접적으로돼지를이용하지않고도실험실내 in vitro 상에서평가가능하고, 또한, T 세포가추출된세포의개체와동일개체(autologous) 혹은동일유전자형을가진다른개체(heterologous)에대한 PRRSV 증식억제를확인할수 있는효과가있어, 백신및 면역증강제의효능평가분야에유용하게이용할수 있다.

Abstract translation: 本发明涉及一种评估T细胞抑制病原体感染细胞增殖能力的方法和一种提供涉及细胞抑制的病原体特异性T细胞信息的方法。 和本发明的细胞的Ť病原体感染的细胞增殖的抑制能力评价方法对体外实验室评估，而无需使用直接猪，也时，T细胞被提取的信元的对象，并且在同一个体（自体）或相同的基因型 本发明可以用于评价疫苗和免疫刺激剂的功效，因为它具有证实对其他异源个体的PRRSV增殖抑制的作用。

公开(公告)号：KR1020140080860A

公开(公告)日：2014-07-01

申请号：KR1020120149343

申请日：2012-12-20

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

Inventor： 송재영 ,  최은진 ,  이창희 ,  이진용 ,  김은주 ,  임성인 ,  이은정 ,  고희영 ,  박경민 ,  정광면

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: G01N33/6857 ,  G01N33/569 ,  G01N33/587 ,  G01N33/6893

Abstract: The present invention relates to a bio probe for detecting swine fever virus, comprising (a) a nanomaterial; (b) an antibody which is specifically combined with an antigen of swine fever virus covalently linked to the surface of the nanomaterial; and (c) a glass fiber, as a solid substrate, having a surface which is modified by the antibody combined nanomaterial. The present invention also relates to a method for diagnosing swine fever virus using the bio probe. According to the present invention, the existence and infection of swine fever virus can be rapidly checked within 10 minutes using a small amount of a sample.

Abstract translation: 本发明涉及一种用于检测猪瘟病毒的生物探针，包括（a）纳米材料; （b）与与纳米材料的表面共价连接的猪瘟病毒的抗原特异性结合的抗体; 和（c）作为固体基质的具有由抗体组合纳米材料修饰的表面的玻璃纤维。 本发明还涉及使用生物探针诊断猪瘟病毒的方法。 根据本发明，可以使用少量的样品在10分钟内快速检查猪瘟病毒的存在和感染。

公开(公告)号：KR1020130063850A

公开(公告)日：2013-06-17

申请号：KR1020110130447

申请日：2011-12-07

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 신연경 ,  송재영 ,  최은진 ,  조우얀 ,  표현미

IPC: C12N7/01 ,  C12N15/44 ,  A61K39/145 ,  A61P31/16

Abstract: PURPOSE: A recombinant baculovirus is provided to protect a swinery from novel swine-origin influenza A(H1N1) and to prevent new or variant virus expressed in a pig. CONSTITUTION: A recombinant baculovirus is prepared by inserting a gene which expresses HA(hemagglutinin), a gene which expresses NA(neuraminidase), and a gene which expresses M1(matrix 1) protein. The HA expressing gene is a gene(GeneBank FJ966082) which expresses HA of influenza virus A/California/04/2009(CA09). The NA expressing gene is a gene(GeneBank FJ966084) which expresses NA of influenzavirus A/California/04/2009(CA09). The M1 protein expressing gene is a gene(GeneBank FJ969513) which expresses M1 protein of influenza virus A/California/04/2009(CA09). A VLP(Virus-Like Particle) for suppressing swine influenza viral infection contains the recombinant baculovirus. A vaccine for suppressing swine influenza viral infection contains the VLP. [Reference numerals] (AA) Tn7L,Tn7R sequence homologous recombination for Baculovirus

Abstract translation: 目的：提供重组杆状病毒以保护猪只免受猪源甲型H1N1流感病毒侵袭，并防止在猪中表达的新病毒或变异病毒。 构成：通过插入表达HA（血凝素）的基因，表达NA（神经氨酸酶）的基因和表达M1（基质1）蛋白质的基因来制备重组杆状病毒。 HA表达基因是表达流感病毒A / California / 04/2009（CA09）的HA的基因（GeneBank FJ966082）。 NA表达基因是表达流感病毒A / California / 04/2009（CA09）的NA的基因（GeneBank FJ966084）。 M1蛋白表达基因是表达流感病毒A / California / 04/2009（CA09）的M1蛋白的基因（GeneBank FJ969513）。 用于抑制猪流感病毒感染的VLP（病毒样颗粒）含有重组杆状病毒。 用于抑制猪流感病毒感染的疫苗包含VLP。 （AA）Tn7L，Tn7R序列同源重组杆状病毒

公开(公告)号：KR1020120055385A

公开(公告)日：2012-05-31

申请号：KR1020100117104

申请日：2010-11-23

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 송재영 ,  최은진 ,  이창희 ,  정호섭 ,  김기성 ,  강도현

IPC: C07D303/12 ,  G01N33/92 ,  C07C57/24

Abstract: PURPOSE: A solid particle having end epoxy radical on lipid bilayer and a manufacturing method thereof are provided to uniformly fix molecules having amino radicals under mild condition. CONSTITUTION: A solid particle having end epoxy radical includes lipid bilayer coated with polyelectrolyte material on a surface. The lipid bilayer comprises a first lipid molecule and a second lipid molecule. The first lipid molecule is diacetylene having the first lipid molecule having epoxy radical in the end. The second lipid molecule is a different lipid molecule from the first lipid molecule. A solid particle before being coated with the polyelectrolyte material is negative charged material. A cationic polyelectrolyte material and electronegative polyelectrolyte material are coated by turns.

Abstract translation: 目的：提供在脂质双层上具有末端环氧基的固体颗粒及其制备方法，以均匀地固定温和条件下具有氨基的分子。 构成：具有末端环氧基的固体颗粒包括在表面上用聚电解质材料涂覆的脂质双层。 脂质双层包含第一脂质分子和第二脂质分子。 第一脂质分子是具有最终具有环氧基的第一脂质分子的乙炔。 第二脂质分子是与第一脂质分子不同的脂质分子。 在用聚电解质材料涂覆之前的固体颗粒是负带电材料。 阳离子聚电解质材料和电负极聚电解质材料被轮转涂覆。

公开(公告)号：KR1020080026064A

公开(公告)日：2008-03-24

申请号：KR1020070094861

申请日：2007-09-18

Applicant: 성균관대학교산학협력단 ,  (주)인비트로플랜트 ,  아주대학교산학협력단 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이석찬 ,  김용성 ,  권명희 ,  김태현 ,  김정선 ,  이건섭 ,  심혜경 ,  박을용 ,  정유철 ,  김기윤 ,  정의정 ,  이우람 ,  김영림 ,  손종남 ,  이승현 ,  송재영 ,  최은진

IPC: A61K38/16 ,  A61P31/12

CPC classification number: C07K16/10 ,  A61K39/00 ,  A61K39/395 ,  A61K2039/505 ,  A61K2300/00 ,  C07K14/47 ,  C07K16/44 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92

Abstract: An antiviral agent is provided to exhibit advantageous effects of selectively degrading foreign nucleic acid chains invaded in an animal cell and have no cytotoxicity to the animal cell, thereby causing no death of the animal cell. An anti-viral agent against animal viruses comprises: a protein which has the binding capacity and the decomposing capacity regarding a foreign nucleic acid chain invading animal cells and shows no toxicity on the animal cells; or a nucleic acid encoding the same protein as an effective ingredient, wherein the protein is recovered from an auto-immune disease induced animal and is an immunoglobulin or a fragment thereof having the binding capacity and the decomposing capacity regarding the nucleic acid chain. Further, the immunoglobulin is an immunoglobulin G.

Abstract translation: 提供抗病毒剂以显示有选择地降解入侵在动物细胞中的外源核酸链并且对动物细胞没有细胞毒性，从而不会导致动物细胞死亡的有利效果。 针对动物病毒的抗病毒剂包括：具有与外来核酸链侵入动物细胞相关的结合能力和分解能力并且对动物细胞无毒性的蛋白质; 或编码与有效成分相同的蛋白质的核酸，其中所述蛋白质是从自身免疫疾病诱导的动物中回收的，并且是具有与所述核酸链相关的结合能力和分解能力的免疫球蛋白或其片段。 此外，免疫球蛋白是免疫球蛋白G.

公开(公告)号：KR100791538B1

公开(公告)日：2008-01-07

申请号：KR1020060050357

申请日：2006-06-05

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

Inventor： 송재영 ,  김병한 ,  탁동섭 ,  최은진 ,  임성인 ,  한규하 ,  주후돈 ,  장병식 ,  이현정 ,  박경민

IPC: C12N15/33 ,  C12N15/09 ,  C12N15/10

Abstract: 본 발명은 돼지콜레라 바이러스 E
rns
단백질 및 이에 특이적으로 반응하는 단클론항체를 이용한 돼지콜레라
항체 감별 진단방법에 관한 것으로, 더욱 상세하게는 국내에서 분리된 다양한 유전형의 돼지콜레라 바이러스(swine fever virus) 중 3종의 E
rns
단백질을 코딩하는 유전자를 배큘로바이러스(Baculovirus)를 이용하여 발현한 재조합 돼지콜레라 바이러스 E
rns
단백질, 및 상기 E
rns
단백질에 특이적으로 반응하는 단클론항체(monoclonal antibody)를 이용하여 돼지콜레라 E
rns
에 대한 항체를 검사하는 방법에 관한 것이다. 본 발명의 단클론항체를 이용하는 경우에 E
rns
에 대한 항체를 검출할 수 있어 야외에서 감염된 돼지콜레라 바이러스 항체를 용이하게 감별 진단할 수 있으며, 또한 본 발명의 진단 방법은, 3종의 재조합 단백질을 동시에 이용하는 경우에 돼지콜레라 바이러스의 모든 유전형에 대해 유전형에 관계없이 일정한 민감도를 나타내므로, 돼지콜레라 바이러스에 대한 항체를 용이하게 검출할 수 있다.
돼지 콜레라 바이러스, 이알엔에스, 단클론항체, 감별 ELISA

公开(公告)号：KR1020070116382A

公开(公告)日：2007-12-10

申请号：KR1020060050357

申请日：2006-06-05

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

Inventor： 송재영 ,  김병한 ,  탁동섭 ,  최은진 ,  임성인 ,  한규하 ,  주후돈 ,  장병식 ,  이현정 ,  박경민

IPC: C12N15/33 ,  C12N15/09 ,  C12N15/10

Abstract: A method for screening and detecting a swine fever virus antibody is provided to easily screen and diagnose infected swine fever virus antibody by using a monoclonal antibody specifically reacting with a recombinant swine fever virus Erns protein and secure constant sensitivity regardless of all genotypes of the swine fever virus when using all three kinds of recombinant protein. A recombinant swine fever virus Erns is prepared by expressing a gene encoding three Erns proteins among swine fever viruses having various genotypes using baculovirus. A method for preparing a monoclonal antibody specifically reacting to the Erns comprises the steps of: (a) constructing a swine fever virus LOM strain-derived Erns recombinant gene using a baculovirus expression system; and (b) producing the monoclonal antibody using the recombinant Erns protein. A method for screening and detecting a swine fever virus antibody comprises the steps of: (a) mixing three kinds of Erns recombinant proteins at proper concentration to prepare an antigen cocktail; (b) after diluting the antigen cocktail into a coating buffer solution, pouring it in a plate to be absorbed into the plate; (c) washing the recombinant antigen not-absorbed into the plate to remove it; (d) adding a sample to be tested to the plat to allow the reaction; (e) washing the sample to be tested not specifically bound to the recombinant Erns antigen protein absorbed into the plate to remove it; (f) adding a monoclonal antibody not being bound to the enzyme but being specifically reacted to the Erns protein to allow the reaction; (g) washing the monoclonal antibody not being bound to the recombinant Erns antigen protein absorbed into the plate to remove it; and (h) adding a substrate reacting with the enzyme to determine the swine fever virus infection.

Abstract translation: 提供了一种筛选和检测猪瘟病毒抗体的方法，通过使用与重组猪瘟病毒Erns蛋白特异性反应的单克隆抗体轻松筛选和诊断感染的猪瘟病毒抗体，无论猪瘟的所有基因型如何 使用全部三种重组蛋白的病毒。 通过使用杆状病毒在具有各种基因型的猪瘟病毒中表达编码三种Erns蛋白质的基因来制备重组猪瘟病毒Erns。 制备与Erns特异性反应的单克隆抗体的方法包括以下步骤：（a）使用杆状病毒表达系统构建猪瘟病毒LOM菌株衍生的Erns重组基因; 和（b）使用重组Erns蛋白产生单克隆抗体。 筛选和检测猪瘟病毒抗体的方法包括以下步骤：（a）以适当浓度混合三种Erns重组蛋白以制备抗原混合物; （b）将抗原混合物稀释到涂布缓冲溶液中后，将其倒入板中吸收到板中; （c）将未吸收的重组抗原洗涤到板中以除去它; （d）将待测试样品加入平板以允许反应; （e）洗涤待测试的样品，未特异性地结合到吸收到板中的重组Erns抗原蛋白质以除去它; （f）加入未结合酶的单克隆抗体，但与Erns蛋白特异性反应以进行反应; （g）洗涤未结合到吸附到板中的重组Erns抗原蛋白的单克隆抗体以除去它; 和（h）加入与酶反应的底物以确定猪瘟病毒感染。