Abstract:
본 발명은 흑염소 반추위 미생물 유래의 엔도글루카나아제 (endo-glucanase) 단백질, 상기 엔도글루카나아제 단백질을 코딩하는 유전자, 상기 유전자를 포함하는 재조합 벡터, 상기 재조합 벡터로 형질전환된 숙주세포, 상기 엔도글루카나아제 단백질 대한 항체, 상기의 재조합 벡터를 숙주세포에 도입하여 엔도글루카나아제를 과발현하는 단계를 포함하는 숙주세포에서 엔도글루카나아제를 대량생산하는 방법, 상기 방법에 의해 제조된 재조합 엔도글루카나아제 단백질, 상기 엔도글루카나아제 단백질 또는 상기 재조합 엔도글루카나아제 단백질을 유효성분으로 포함하는 글루칸 분해 증진용 사료 첨가제 또는 세제 조성물 및 바이오매스 물질에 상기 엔도글루카나아제 단백질 또는 상기 재조합 엔도글루카나아제 단백질을 첨가하여 가수분해시키는 단계를 포함하는 바이오연료 (Biofuel)의 제조 방법을 제공한다.
Abstract:
The present invention relates to a kit for detecting an abdominal adipose tissue of Korean beef using abdominal adipose tissue specific expression gene TBX152 and ZIC1 of the Korean beef, and a detecting method using the same. More specifically, the present invention relates to the kit for detecting the abdominal adipose tissue of the Korean beef by investigating an expression ratio of the TBX152 and ZIC1 genes in an object to be tested, and the detecting method using the same. According to the present invention, the commercialization of the kit for detecting an abdominal adipose specific gene is available. Also, high-quality meat can be produced through the induction of specific fat accumulation within beef loin of the Korean beef, and a body composition control technique can be developed by controlling the contents of fat of the Korean beef. Therefore, body fat control information can be applied to other species.
Abstract:
본 발명은 돼지의 육질 진단을 위한 유전자 마커 및 진단방법에 관한 것이다. 보다 상세하게는, 돼지 종돈을 선발하는 경우 정확성을 높이고 유전능력 개량을 극대화할 수 있는 표지인자 도움 선발(marker assisted selection, MAS)에 활용할 수 있는 표지인자 및 이의 이용방법에 관한 것이다. 본 발명에 따른 표지인자를 활용하면 표현형가에 의한 선발효과보다 25~35% 더 정확한 유전능측정이 가능하여 종돈의 유전능력 개량량을 획기적으로 늘릴 수 있으며, 종돈 수입은 1,800여두로(년 1,836두)로 연간 약 40억원 정도의 수입 규모로 약 30% 대체 시(550두) 연간 약 10억원의 절감 효과가 있을 것으로 기대된다.
Abstract:
PURPOSE: A product of cel10-KG41 gene, endoxylanase, is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoxylanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes endoxylanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoxylanase from the host cell comprises a step of overexpressing endoxylanase by transducing the recombinant vector to the host cells.
Abstract:
PURPOSE: A protein of cel3-KG39 gene, beta-glucosidase, which is acquired by searching glucosidase gene from a ruminant stomach microorganism of a black goat is provided to be used for a feed additive and a detergent composition, and to develop biofuel. CONSTITUTION: A beta-glucosidase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes beta-glucosidase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of beta-glucosidase in the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing beta-glucosidase. [Reference numerals] (A) Front domain area; (B) Rear domain area
Abstract:
PURPOSE: A product of cel-KG35 gene, endoglucanase, is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase in the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing endoglucanase.
Abstract:
PURPOSE: A product of Cel9-KG10 gene, endoglucanase, is provided to be used as a feed additive and a detergent composition and to efficiently develop biofuel by finding a cellulase gene from the microorganism. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. The gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing endoglucanase.
Abstract:
PURPOSE: Endo-1,4-beta-xylanase which is a product of cel10-KG04 gene is provided to be used for a feed additive and a detergent composition, and to develop biofuel. CONSTITUTION: Endo-1,4-beta-xylanase derived from ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes endo-1,4-beta-xylanase. A recombinant vector contains the gene. A host cell is transformed with the recombinant vector. A method for producing a large amount of endo-1,4-beta-xylanase from the host cell comprises a step of transducing a recombinant vector to the host cell and overexpressing endo-1,4-beta-xylanase.
Abstract:
본 발명은 반추동물에 공급되는 L-카르니틴 함유 사료첨가제에 있어서, L-카르니틴을 에틸셀룰로오즈와 아미노알킬메트아크릴레이트 중합체로 이중피복한 펠렛제제를 포함함을 특징으로 하는 반추위 동물급여용 사료첨가제를 제공한다. 또한, 본 발명은 L-카르니틴을 에틸셀룰로오즈와 아미노알킬메트아크릴레이트 중합체로 이중피복하여 얻어지는 반추위 동물 급여용 사료첨가제를 반추동물에 급여함으로써 육용 또는 우유용 반추동물 및 그 생산물에서 L-카르니틴의 함량을 증진시키는 방법을 제공한다. 반추위, 카르니틴, 바이패스