公开(公告)号：KR1020150056448A

公开(公告)日：2015-05-26

申请号：KR1020140115688

申请日：2014-09-01

Applicant: 삼성전자주식회사

Inventor： 송지윤 ,  강창덕 ,  박준성 ,  김성수 ,  박영경 ,  이성행 ,  이소영 ,  이주영 ,  조광명 ,  이우용

IPC: C12N1/19 ,  C12N15/52 ,  C12P7/56

Abstract: 그모세포에비하여아세트알데히드를아세틸-CoA로전환하는것을촉매하는효소의활성및 피루베이트를락테이트로전환하는것을촉매하는효소의활성증가되어있는, 락테이트생산능을갖는유전적으로조작된효모세포, 그를제조하는방법및 그를이용하여락테이트를생산하는방법을제공한다.

Abstract translation: 提供了具有乳酸生产能力的遗传工程酵母细胞，其包括催化从乙醛转化为乙酰辅酶A的酶和催化从丙酮酸向乳酸转化的酶，其活性比酵母细胞的亲本细胞增加 ，酵母细胞的制造方法以及使用酵母细胞的乳酸盐的制造方法。

公开(公告)号：KR1020150018232A

公开(公告)日：2015-02-23

申请号：KR1020130094891

申请日：2013-08-09

Applicant: 삼성전자주식회사

Inventor： 김성수 ,  이소영 ,  강창덕 ,  이주영 ,  조광명

IPC: C12N1/19 ,  C12N15/53 ,  C12N15/81 ,  C12P7/56

CPC classification number: C12P7/56 ,  C12N9/0006 ,  C12N15/81 ,  C12Y101/01027 ,  Y02P20/52

Abstract: The present invention is to provide a yeast cell with improved lactate production ability, and a method for producing lactate using the same. More specifically, provided is a yeast cell which has improved activity of lactate dehydrogenase which converts pyruvate into lactate and includes an introduced gene coding the lactate dehydrogenase. In addition, provided is the method for producing lactate using the yeast cell.

Abstract translation: 本发明提供一种具有改善的乳酸盐生产能力的酵母细胞，以及使用该酵母细胞产生乳酸盐的方法。 更具体地，提供了具有改善的乳酸脱氢酶活性的酵母细胞，其将丙酮酸转化成乳酸，并且包括编码乳酸脱氢酶的引入基因。 此外，提供使用酵母细胞产生乳酸盐的方法。

公开(公告)号：KR1020140100901A

公开(公告)日：2014-08-18

申请号：KR1020140012793

申请日：2014-02-04

Applicant: 삼성전자주식회사

Inventor： 김재영 ,  강진규 ,  강창덕 ,  김성수 ,  박재찬 ,  유병조 ,  이주영 ,  임휘섭

IPC: C12N1/19 ,  C12P7/56

CPC classification number: C12P7/56 ,  C07K14/395

Abstract: An example of the present invention relates to a microorganism with improved lactic acid productivity, which improves the lactic acid productivity when overexpressing a lactic acid transporter which is a protein transporting a lactic acid to the inside or the outside of the cell. Moreover, the lactic acid productivity is improved when the activity of a lactic acid oxidase is inhibited and the activity of pyruvate deoxidant enzyme. A lactic acid production yield can be improved by using the transformed microorganism manufactured by the same, thereby being capable of useful industrial application.

Abstract translation: 本发明的一个实例涉及乳酸生产率提高的微生物，当将乳酸转运蛋白过量表达时，乳酸转运蛋白是转运到细胞内部或外部的蛋白质，其提高乳酸生产率。 此外，当抑制乳酸氧化酶的活性和丙酮酸脱氧酶的活性时，乳酸生产率提高。 可以通过使用由其制造的转化微生物来提高乳酸产量，从而能够有用的工业应用。

公开(公告)号：KR1020140094757A

公开(公告)日：2014-07-31

申请号：KR1020130007091

申请日：2013-01-22

Applicant: 삼성전자주식회사 ,  중앙대학교 산학협력단

Inventor： 김재영 ,  강진규 ,  강창덕 ,  김성수 ,  이주영 ,  강현아 ,  박재찬 ,  임휘섭 ,  추진호

IPC: C12N15/63 ,  C12N15/81 ,  C12Q1/68

CPC classification number: C12N15/81 ,  C12N15/905 ,  C12N15/63 ,  C12N15/66 ,  C12N15/902 ,  C12Q1/6865

Abstract: The present invention relates to: a promoter specific homologous part which is homologous with a part of the promoter of a target gene; a marker gene which is connected with the promoter specific homologous part in order to be able to operate; a cassette, for causing a defect of the target gene, which is connected to the downstream of the marker gene and includes a gene specific homologous part which is homologous with at least one target gene; and a gene manipulation method which uses the cassette. The efficient gene manipulation and selection of a cell are possible by using the cassette and the gene manipulation method using the cassette.

Abstract translation: 本发明涉及：与靶基因的一部分启动子同源的启动子特异性同源部分; 与启动子特异性同源部分连接以便能够操作的标记基因; 用于引起靶标基因的缺陷，其与所述标记基因的下游连接并且包括与至少一种靶基因同源的基因特异性同源部分; 以及使用该盒的基因操作方法。 通过使用盒子和使用盒子的基因操作方法，可以有效地进行细胞的基因操作和选择。