-
公开(公告)号：KR1020160116767A
公开(公告)日：2016-10-10
申请号：KR1020150044994
申请日：2015-03-31
Applicant: 아주대학교산학협력단
Abstract: 본발명은재조합벡터로형질전환된스테비올바이오사이드생산용재조합미생물및 상기재조합미생물을이용한스테비올바이오사이드의생산방법에관한것이다. 본발명에따른재조합벡터로형질전환된재조합미생물은스테비아의감미성분으로서스테비올의배당체인스테비올바이오사이드를다량으로생산하는우수한효과가있다. 따라서, 본발명의재조합미생물을이용할경우, 스테비아의추출물로부터직접스테비올바이오사이드를분리하는것이아닌, 미생물에도입된재조합유전자에의해대사경로를조작함으로써, 간단한공정만으로도스테비올바이오사이드를대량으로제공할수 있어, 이를이용하는천연감미료, 음료등의식품산업에유용하게이용될수 있다.
-
32.发明授权메발로네이트 또는 메발로노락톤 생성능을 가지는 재조합 미생물, 및 이를 이용한 메발로네이트 또는 메발로노락톤의 제조방법 有权具有甲羟戊酸或甲羟戊酸内酯生产能力的重组微生物及使用其制备甲羟戊酸或甲羟戊酸的方法
公开(公告)号：KR101625898B1
公开(公告)日：2016-06-01
申请号：KR1020140061139
申请日：2014-05-21
Applicant: 아주대학교산학협력단
Abstract: 본발명은메발로네이트또는메발로노락톤생성능을가지는재조합미생물, 및이를이용한메발로네이트또는메발로노락톤의제조방법에관한것이다. 본발명에따른엔테로코커스킹세종엔시스 Gf1(Enterococcus Kingsejongensis Gf1) 및엔테로코커스안타크티큠 Gf2(Enterococcus Antarticum Gf2) 유래의 mvaS 및 mvaE 유전자를도입한재조합미생물을이용할경우, 간단한공정만으로도메발로네이트또는메발로노락톤을효율적으로대량생산할수 있다.
-
公开(公告)号：KR101533355B1
公开(公告)日：2015-07-06
申请号：KR1020130043540
申请日：2013-04-19
Applicant: 아주대학교산학협력단
CPC classification number: C12Y505/01013 , C12N9/0073 , C12N9/1085 , C12N9/88 , C12N9/90 , C12P5/007 , C12P7/42 , C12P15/00 , C12Y114/13078 , C12Y205/01029 , C12Y402/03019
Abstract: 본발명은스테비오사이드의전구체인카우린, 카우린산또는스테비올생성능을가지는재조합미생물및 이를이용한카우린, 카우린산또는스테비올의제조방법에관한것이다. 본발명에따르면스테비아의추출물로부터직접분리하는것이아닌, 미생물에도입된재조합유전자에의해대사경로를조작함으로써, 간단한공정만으로도천연감미료인스테비오사이드의전구체인카우린, 카우린산또는스테비올을다량으로제공할수 있다.
-
-
公开(公告)号：KR1020130048058A
公开(公告)日：2013-05-09
申请号：KR1020110112974
申请日：2011-11-01
Applicant: 아주대학교산학협력단
Abstract: PURPOSE: Recombinant E. coli with reconstructed sucrose metabolic pathways is provided to be used in a microorganism-based industry by transforming E.coli which produces a secondary metabolite using only sucrose as a carbides source. CONSTITUTION: Sucrose-using E.coli is a reconstructed PTS(phosphoenolpyruvate (PEP)-dependent phosphotransferase transport system) system or non-PTS system. The PTS system is prepared by transduction of sucrose phosphotransferase, sucrose hydrolase, and fructokinase. The non-PTS system is prepared by transduction of sucrose permease, sucrose hydrolase or phophorylase, and fructokinase. The PTS-syste-introduced E.coli is prepared by transformation with pAC-AAK plasmid which expresses scrA, sacA, and sack genes. The gene is derived from Staphylococcus xylosus and Lactobacillus plantarum.
Abstract translation: 目的：重组蔗糖代谢途径的重组大肠杆菌提供用于基于微生物的工业，通过转化大肠杆菌，其仅使用蔗糖作为碳化物源产生次级代谢物。 构成：使用蔗糖的大肠杆菌是重建的PTS（磷酸烯醇丙酮酸（PEP）依赖性磷酸转移酶转运系统）系统或非PTS系统。 PTS系统是通过转导蔗糖磷酸转移酶，蔗糖水解酶和果糖激酶来制备的。 非PTS系统通过蔗糖通透酶，蔗糖水解酶或磷酸化酶和果糖激酶的转导来制备。 通过用表达scrA，sacA和sack基因的pAC-AAK质粒转化来制备PTS-系统引入的大肠杆菌。 该基因来源于木糖葡萄球菌和植物乳杆菌。
-
Patent Agency Ranking
公开(公告)号：KR1020130043382A
公开(公告)日：2013-04-30
申请号：KR1020110107450
申请日：2011-10-20
Applicant: 아주대학교산학협력단
Abstract: PURPOSE: A recombinant microbe with menaquinone productivity, and a method for preparing menaquinone using the same are provided to induce excessive production of menaquinone. CONSTITUTION: A recombinant microbe with menaquinone productivity over-expresses one gene selected from the group consisting of UbiE(ubiquinone-8/menaquinone-8 biosynthesis methyltransferase), MenA(1,4-dihydroxy-2-naphthoate octaprenyltransferase), MenB (naphthoate synthase), MenC(osuccinylbenzoate-CoA synthase), MenD(2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylatesynthase/2-oxoglutarate decarboxylase), MenE(o-succinylbenzoic acid-CoA ligase), MenF(menaquinonespecificisochorismate synthase), ispA (geranyltranstransferase), dxr(1-deoxy-D-xylulose 5-phosphatesynthase), and idi(isopentyl diphosphate isomerase). A method for preparing menaquinone comprises a step of culturing the recombinant microbe, and a step of collecting menaquinone from a culture medium of the recombinant microbe.
Abstract translation: 目的：提供具有美萘醌生产力的重组微生物，以及使用其制备甲萘醌的方法以诱导过量生产甲萘醌。 构成：具有美萘醌生产力的重组微生物过表达一种选自UbiE（泛醌-8 /甲萘醌-8生物合成甲基转移酶），MenA（1,4-二羟基-2-萘甲酸八苯酯转移酶），MenB（萘甲酸合酶） ），MenC（异琥珀酰基苯甲酸酯-CoA合酶），MenD（2-琥珀酰基-6-羟基-2,4-环己二烯-1-羧酸合酶/ 2-氧戊二酸脱羧酶），MenE（邻琥珀酰基苯甲酸-CoA连接酶），MenF（Menaquinonespecificisochorismate 合成酶），ispA（香叶基转移酶），dxr（1-脱氧-D-木酮糖-5-磷酸酯酶）和idi（异戊基二磷酸异构酶））。 制备甲萘醌的方法包括培养重组微生物的步骤和从重组微生物的培养基中收集甲萘醌的步骤。
-
公开(公告)号：KR1020120140526A
公开(公告)日：2012-12-31
申请号：KR1020110060320
申请日：2011-06-21
Applicant: 아주대학교산학협력단
Abstract: PURPOSE: A shuttle vector comprising plasmid from lactic acid bacteria is provided to stably express a gene or protein and to be able to reproduce over 100 generations due to a stable method of theta-typed reproduction. CONSTITUTION: Plasmid pMBLT00 comprises the sequence number marked sequence number 1(SEQ ID NO:1). Plasmid pMBLT00 is separated Leuconostoc mesenteroides. A shuffle vector capable of reproduction both in LAB-E-coli and lactic acid bacteria comprises sequence number marked the sequence number 1 and produced by using plasmid pMBLT00 and transformation vector from LAB-E-coli. The transformation vector from LAB-E-coli is pUC19. The shuffle vector has a method of theta-typed reproduction. A transformant transformed from the shuffle vector is LAB-E-coli or lactic acid bacteria. Lactic acid bacteria are Lactococcus, Leuconostoc or Pediococcus. A method of reproduction comprises the following steps. The shuffle vector capable of reproduction both in LAB-E-coli and lactic acid bacteria is made by using plasmid pMBLT00 with the sequence number marked the sequence number 1 and transformation vector from LAB-E-coli(a). The recombinant vector is made combining the shuffle vector comprising a target gene which codes target proteins(b). The recombinant vector is injected into microorganism.
Abstract translation: 目的：提供包含来自乳酸菌质粒的穿梭载体，以稳定表达基因或蛋白质，并且由于θ型繁殖的稳定方法能够再生100代以上。 构成：质粒pMBLT00包含标记序列号1（SEQ ID NO：1）的序列号。 质粒pMBLT00分离明串珠菌肠炎菌。 能够在LAB-大肠杆菌和乳酸菌中繁殖的洗牌载体包含标记序列号1的序列号，并使用质粒pMBLT00和来自LAB-大肠杆菌的转化载体产生。 来自LAB-大肠杆菌的转化载体是pUC19。 洗牌矢量具有θ型再现的方法。 从洗牌载体转化的转化体是LAB-大肠杆菌或乳酸菌。 乳酸菌是乳球菌属，明串珠菌属或类球菌属。 一种再现方法包括以下步骤。 通过使用序列号为序列号1的质粒pMBLT00和来自LAB-大肠杆菌（a）的转化载体，制备能够在LAB-大肠杆菌和乳酸菌中繁殖的洗发液。 将重组载体组合包含编码靶蛋白（b）的目标基因的洗牌载体。 将重组载体注入微生物。
-
公开(公告)号：KR101669041B1
公开(公告)日：2016-10-25
申请号：KR1020150045009
申请日：2015-03-31
Applicant: 아주대학교산학협력단
Abstract: 본발명은재조합벡터로형질전환된재조합미생물, 상기재조합미생물에돌연변이를유발시킨돌연변이미생물, 및상기재조합미생물또는돌연변이미생물을이용한스테비오사이드의생산방법에관한것이다. 본발명에따른재조합벡터로형질전환된재조합미생물또는상기재조합미생물에돌연변이를유발시킨돌연변이미생물은스테비오사이드를다량으로생산하는우수한효과가있다. 따라서, 스테비아의추출물로부터직접스테비오사이드를분리하는것이아닌, 미생물에도입된재조합유전자에의해대사경로를조작함으로써, 간단한공정만으로도스테비오사이드를대량으로제공할수 있어, 상기스테비오사이드를이용하는천연감미료, 음료등의식품산업에유용하게이용될수 있다.
-
公开(公告)号：KR1020160116765A
公开(公告)日：2016-10-10
申请号：KR1020150044981
申请日：2015-03-31
Applicant: 아주대학교산학협력단
Abstract: 본발명은재조합벡터로형질전환된스테비올모노사이드생산용재조합미생물및 상기재조합미생물을이용한스테비올모노사이드의생산방법에관한것이다. 본발명에따른재조합벡터로형질전환된재조합미생물은스테비아의감미성분으로서스테비올의배당체인스테비올모노사이드를다량으로생산하는우수한효과가있다. 따라서, 본발명의재조합미생물을이용할경우, 스테비아의추출물로부터직접스테비올모노사이드를분리하는것이아닌, 미생물에도입된재조합유전자에의해대사경로를조작함으로써, 간단한공정만으로도스테비올모노사이드를대량으로제공할수 있어, 이를이용하는천연감미료, 음료등의식품산업에유용하게이용될수 있다.
-
40.发明授权젖산 내성 강화능 및 고생산능을 갖는 루코노스톡 메센테로이데스 LMA92A 균주 및 이를 이용한 젖산 생산 방법 有权LMA92A具有增强乳酸耐受性和产生能力的肠膜明串珠菌LMA92A和使用其制备乳酸的方法
公开(公告)号：KR101617883B1
公开(公告)日：2016-05-04
申请号：KR1020140067555
申请日：2014-06-03
Applicant: 아주대학교산학협력단
Abstract: 본발명은젖산내성강화능및 고생산능을갖는루코노스톡메센테로이데스 LMA92A 균주, 상기균주를이용하는젖산의생산방법및 젖산내성강화균주의제조방법에관한것이다. 본발명에따른신규한루코노스톡메센테로이데스 LMA92A 균주는모균주에비해젖산에대한내성이강화되고 D형젖산생산능이현저하게우수하여, 젖산및 PLA 상업화기술뿐만아니라 D형젖산을원료로사용하는각종제품의생산성을향상시키는데널리활용될수 있다.
-
-
-
-
-
-
-
-
-
Search Results
57
records
(took 0.029 seconds)
