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31.发明授权
公开(公告)号：KR101457520B1
公开(公告)日：2014-11-03
申请号：KR1020120088843
申请日：2012-08-14
Applicant: 한국과학기술연구원
CPC classification number: A61K36/04 , A61K2236/39
Abstract: 본 발명은 항바이러스 활성을 갖는 새빨간검둥이 추출물을 유효성분으로 함유하는 조성물을 제공한다. 또한, 새빨간검둥이를 유기용매로 추출한 후 감압증류하여 추출물을 얻는 단계; 상기 단계에서 얻어진 유기용매 추출물을 용매의 극성의 차이를 이용하여 분획하여 분획추출물을 얻는 단계; 및 상기 단계에서 얻어진 분획추출물을 크로마토그래피로 분리 및 정제하는 단계;를 포함하는 폴리브로모카테콜의 분리방법을 제공한다. 본 발명에 의하면 인간 리노바이러스에 대한 억제능이 우수하며 천연물 유래이기 때문에 안전하고 독성이 적은 항바이러스 물질을 제공할 수 있다. 또한, 새빨간검둥이로부터 폴리브로모카테콜 화합물을 손쉽게 분리할 수 있어, 바이러스 질환에 대한 예방 및 치료제로 사용가능한 약학 조성물을 제공할 수 있다.
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公开(公告)号：KR101371361B1
公开(公告)日：2014-03-07
申请号：KR1020120137304
申请日：2012-11-29
Applicant: 한국과학기술연구원
Abstract: The present invention relates to a method for quantifying fatty oil using means IV, and more specifically, to a method for quantifying fatty oil in a living body using means IV. According to one embodiment of the present invention, liquid chromatography (Nanospace SI-2, Shiseido co. Ltd, Tokyo, Japan) with high performance using a sunfire C8 column is used to separate an analyte (triolein, diolein, and monoolein) from an extracted solution. [Reference numerals] (AA) Weight of oil(mg) converted to Sudan IV; (BB) Weight of oil(mg) analyzed to LC/MS; (CC) Sudan recovery rate/Oil recovery rate; (DD) Amount of injected oil(쨉L)
Abstract translation: 本发明涉及使用方法IV定量脂肪油的方法，更具体地，涉及使用方法IV定量生物体中的脂肪油的方法。 根据本发明的一个实施方案，使用具有高性能的Sunfire C8柱的液相色谱（Nanospace SI-2，Shiseido co。Ltd，Tokyo，Japan）将分析物（三油酸甘油酯，二油烯和单油酸酯）从 提取溶液。 （附图标记）（AA）转换为苏丹的油重量（mg）IV; （BB）油液重量（mg）分析至LC / MS; （CC）苏丹回收率/回收率; （DD）注油量（쨉L）
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33.发明公开
公开(公告)号：KR1020130135424A
公开(公告)日：2013-12-11
申请号：KR1020120058960
申请日：2012-06-01
Applicant: 한국과학기술연구원
IPC: C07F5/02 , C07D249/06 , B01J23/72
CPC classification number: B01J23/72 , B01J27/128 , C07D249/06 , C07F5/02
Abstract: The present invention relates to a novel potassium organo-1H-1,2,3-triazole-4-yltrifluoroborate derivative and a manufacturing method thereof. The novel potassium organo-1H-1,2,3- triazole-4-yltrifluoroborate derivative according to the present invention can be usefully used in the manufacture of various kinds of organo 1,2,3-triazole through a general carbon-carbon coupling reaction utilizing a metallic catalyst such as Suzuki-Miyaura coupling, and can be applied to an organic combination reaction, an optical organic material manufacture, medicine production, a medical diagnosis material, a biocompatible material and a bioactive natural material. The manufacturing method for the potassium organo-1H-1,2,3-triazole-4-yltrifluoroborate derivative according to the present invention can manufacture the stable potassium organo-1H-1,2,3-triazole-4-yltrifluoroborate derivative with a single reaction. Accordingly, a manufacturing process is fast, convenient, efficient and economic.
Abstract translation: 本发明涉及一种新型的有机 - 1H-1,2,3-三唑-4-基三氟硼酸钾衍生物及其制造方法。 根据本发明的新型有机基团1H-1,2,3-三唑-4-基三氟硼酸衍生物可用于通过一般碳 - 碳偶联剂制备各种有机-1,2,3-三唑 利用金属催化剂如Suzuki-Miyaura偶联的反应，可以应用于有机组合反应，光学有机材料制造，医药生产，医学诊断材料，生物相容性材料和生物活性天然材料。 本发明的有机-1H-1,2,3-三唑-4-基三氟硼酸钾衍生物的制造方法可以制造稳定的有机-1H-1,2,3-三唑-4-基三氟硼酸钾衍生物， 单反应。 因此，制造过程快速，方便，高效，经济。
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公开(公告)号：KR101260453B1
公开(公告)日：2013-05-06
申请号：KR1020110095963
申请日：2011-09-22
Applicant: 한국과학기술연구원
Abstract: 본발명은생체시료내 스테로이드호르몬들의수산화대사에관여하는여러종류의시토크롬 P450 효소들의활성을질량분석기를이용하여동시에측정및 비교할수 있는분석방법에관한것이다. 본발명은소변과같은생체시료로부터약물복용에따른스테로이드호르몬대사와관련이있는여러종류의시토크롬 P450 효소들의활성도변화를그 대사산물들간의농도비율로나타냄으로써한 번의전처리와분석으로동시에측정할수 있다. 따라서특정약물복용에따른시토크롬 P450 효소활성도변화를통한약물효능평가및 대사기전을총체적으로평가할수 있고, 약물유전체기반의시토크롬 P450 효소분석과의교차검증(cross-validation)이가능한분석기술로도활용할수 있다. 또한, 이들의축척된정보들은신약개발및 약물대사뿐만아니라시토크롬 P450 효소들의활성과관련되는질환등의연구에있어서광범위하게적용될수 있다.
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公开(公告)号：KR1020130032198A
公开(公告)日：2013-04-01
申请号：KR1020110095963
申请日：2011-09-22
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A simultaneous assay method of multiplex cytochrome P450 enzyme activity is provided to simultaneously measure the activity change of various cytochrome P450 enzymes and to study diseases related to the cytochrome P450 enzyme activity. CONSTITUTION: A simultaneous assay method of multiplex cytochrome P450 enzyme activity comprises: a step of simultaneously extracting steroid hormone metabolic products including male hormones, female hormones, corpus luteum hormones, sterol, and adrenal cortical hormones from urine; a step of re-extracting the extracts using an organic solvent and performing trimethyl silyl derivatization; and a step of measuring the concentration of steroid hormone biometabolic products in the urine by gas chromatography-mass spectrometer. [Reference numerals] (AA) Before dosing a drug; (BB) After dosing a drug;
Abstract translation: 目的：提供多重细胞色素P450酶活性的同时测定方法，以同时测量各种细胞色素P450酶的活性变化，并研究与细胞色素P450酶活性相关的疾病。 构成：多重细胞色素P450酶活性的同时测定方法包括：从尿中同时提取包括雄性激素，雌激素，黄体激素，固醇和肾上腺皮质激素的类固醇激素代谢产物的步骤; 使用有机溶剂重新萃取提取物并进行三甲基甲硅烷基衍生化的步骤; 以及通过气相色谱 - 质谱仪测量尿中类固醇激素生物代谢产物浓度的步骤。 （AA）给药前给药; （BB）给药药物后;
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Patent Agency Ranking
公开(公告)号：KR101200423B1
公开(公告)日：2012-11-12
申请号：KR1020110047673
申请日：2011-05-20
Applicant: 한국과학기술연구원
IPC: G01N30/72 , G01N30/02 , G01N33/74 , G01N33/487
Abstract: PURPOSE: A high sensitive analyzing method of estrogen metabolites in urine and blood obtained from postmenopausal women is provided to verify the overall distribution of estrogen metabolism and to be used for overcoming diseases related to estrogen metabolism. CONSTITUTION: A high sensitive analyzing method of estrogen metabolites in urine and blood obtained from postmenopausal women includes the following steps: estrogen is simultaneously extracted from urine and blood obtained from postmenopausal women and is hydrolyzed; the hydrolyzed product is alkoxy carbonylated using C1-4 alkyl chloroformate, and the resultant product is re-extracted using an organic solvent to obtain a re-extract; and the re-extract is acyl-derivated, and the concentration of estrogen is measured in the acyl-derivated product using a gas chromatography-mass spectrometry. The organic solvent is one or the mixture of normal hexane, normal pentane, butyl methyl ether, and ethyl acetate.
Abstract translation: 目的：提供从绝经后妇女获得的尿液和血液中雌激素代谢物的高灵敏度分析方法，以验证雌激素代谢的总体分布，并用于克服与雌激素代谢相关的疾病。 构成：从绝经后妇女获得的尿液和血液中雌激素代谢物的高灵敏度分析方法包括以下步骤：从绝经后妇女获得的尿液和血液中同时提取雌激素，并进行水解; 水解产物是使用氯甲酸C 1-4烷基羰基化烷氧基，所得产物用有机溶剂再萃取，得到再提取物; 并且再提取物是酰基衍生的，并且使用气相色谱 - 质谱法在酰基衍生的产物中测量雌激素的浓度。 有机溶剂是一种或正己烷，正戊烷，丁基甲基醚和乙酸乙酯的混合物。
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公开(公告)号：KR101188714B1
公开(公告)日：2012-10-09
申请号：KR1020110039268
申请日：2011-04-27
Applicant: 한국과학기술연구원
CPC classification number: G01N30/72 , G01N30/64 , G01N2030/8813 , G01N2030/8822 , G01N2030/8827
Abstract: PURPOSE: A method for simultaneously detecting nucleotides in blood plasma using a capillary electrophoresis-mass spectrometer is provided to effectively separate nine kinds of nucleotides from a blood plasma specimen by securing optimal analyzing conditions of the capillary electrophoresis-mass spectrometer. CONSTITUTION: A method for simultaneously detecting nucleotides in blood plasma includes the following: protein is removed from the blood plasma; the blood plasma without the protein passes through a micelle electrokinetically capillary chromatography column which is adjusted with a surfactant containing developing solvent; and the treated blood plasma is analyzed by a capillary electrophoresis-mass spectrometer. The protein is removed using 50-70% of an ethanol solution containing ethylene diamine tetraacetic acid(EDTA). The concentration of the EDTA is in a range between 1 and 5 mM. The surfactant is a cationic surfactant. The developing solvent is an ammonium acetate buffer solution. The concentration of the ammonium acetate buffer solution is in a range between 10 and 60 nM.
Abstract translation: 目的：提供使用毛细管电泳 - 质谱仪同时检测血浆中核苷酸的方法，通过确保毛细管电泳 - 质谱仪的最佳分析条件，有效地将9种核苷酸与血浆标本分离。 构成：同时检测血浆中核苷酸的方法包括：从血浆中除去蛋白质; 没有蛋白质的血浆通过胶束电动毛细管色谱柱，其用含显影溶剂的表面活性剂调节; 并通过毛细管电泳 - 质谱仪分析处理过的血浆。 使用含有乙二胺四乙酸（EDTA）的50-70％的乙醇溶液除去蛋白质。 EDTA的浓度在1至5mM的范围内。 表面活性剂是阳离子表面活性剂。 显影溶剂为乙酸铵缓冲溶液。 乙酸铵缓冲溶液的浓度在10至60nM的范围内。
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公开(公告)号：KR1020120035471A
公开(公告)日：2012-04-16
申请号：KR1020100096995
申请日：2010-10-05
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A method for analyzing metabolic differentiation of hair samples based on quantitative steroid signature is provided to evaluate endocrine disease and biological changes related to steroid hormones. CONSTITUTION: A method for analyzing metabolic differentiation of hair samples includes the following: hair samples are decomposed based on ultrasound wave; steroid hormone biological metabolites including androgen, progesterone, sterol, and adrenocortical hormones are simultaneously extracted; steroid hormone is re-extracted from the metabolites using copolymer absorber; the re-extracted product is trimethylsilyl derivatizated; and the concentration of the metabolites is measured based on a gas chromatography-mass spectrometry.
Abstract translation: 目的：提供一种基于定量类固醇特征分析头发样本代谢分化的方法，以评估与类固醇激素相关的内分泌疾病和生物学变化。 构成：分析头发样本代谢分化的方法包括：头发样品根据超声波分解; 同时提取类固醇激素生物代谢物，包括雄激素，孕激素，固醇和肾上腺皮质激素; 使用共聚物吸收剂从代谢物中再次提取类固醇激素; 再提取产物是衍生的三甲基甲硅烷基化物; 并且基于气相色谱 - 质谱法测量代谢物的浓度。
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公开(公告)号：KR1020110130071A
公开(公告)日：2011-12-05
申请号：KR1020100049515
申请日：2010-05-27
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A method for simultaneously analyzing cholesterol, oxysterol, and bile acid in urine is provided to treat diseases and to prevent side effect. CONSTITUTION: A method for simultaneously detecting cholesterol, oxysterol, and bile acid in urine comprises a step of TMS(trimethylsilylation) derivatization of cholesterol, oxysterol, and bile acid contained in a urine sample. The simultaneous detection comprises: a process of treating enzyme to the urine sample for hydrolyzation; and a process of solvent extraction. The enzyme is beta-glucuronidase or arylsulfatase. They used solvent is a mixture solution of ethyl acetate and n-hexane, ethyl ether, or t-butyl methyl ether.
Abstract translation: 目的：同时分析尿中胆固醇，胆固醇和胆汁酸的方法，用于治疗疾病和预防副作用。 构成：同时检测尿液中胆固醇，氧固醇和胆汁酸的方法包括在尿样中含有胆固醇，氧固醇和胆汁酸的TMS（三甲基甲硅烷基化）衍生化步骤。 同时检测包括：将酶处理至尿样进行水解的过程; 和溶剂萃取过程。 酶是β-葡糖苷酸酶或芳基硫酸酯酶。 使用溶剂是乙酸乙酯和正己烷，乙醚或叔丁基甲基醚的混合溶液。
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公开(公告)号：KR101028042B1
公开(公告)日：2011-04-08
申请号：KR1020090043849
申请日：2009-05-20
Applicant: 한국과학기술연구원
IPC: G01N33/493 , G01N30/12 , G01N30/72
Abstract: 생체 뇨 시료 내 휘발성 유기 화합물(VOC) 대사체 농도 분석 방법으로서, 생체 뇨 시료로부터 휘발성 유기 화합물 대사체들을 추출하기 위하여 생체 뇨 시료의 pH를 산성으로 조절하고, 산성으로 조절된 생체 뇨 시료로부터 음이온 교환 카트리지를 이용하여 휘발성 유기 화합물 대사체들을 고체상 추출하는 방법을 제공한다. 본 발명의 일실시예에 따른 분석 방법을 이용함으로써, 적은 양의 생체 뇨 시료를 이용해서, 뇨 시료에서의 주요 VOC 대사체들의 농도를 동시에 정확하게 분석할 수 있다. 분석된 VOC 대사체들의 농도를 이용하여, 인체 내 VOC 유입에 따른 영향을 판별하기 위한 지표로 활용할 수 있다.
뇨 시료, 휘발성 유기 화합물, 대사체, 고체상 추출
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