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    2'-O-TETRAHYDROPYRANYLNUCLEOSIDE DERIVATIVE AND ITS PREPARATION
    31.
    发明专利
    2'-O-TETRAHYDROPYRANYLNUCLEOSIDE DERIVATIVE AND ITS PREPARATION 失效

    公开(公告)号：JPH01224391A

    公开(公告)日：1989-09-07

    申请号：JP4927388

    申请日：1988-03-02

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： FURUSAWA KIYOTAKA ,  SAKAI TSUKASA

    IPC: C07H19/067 ,  C07H19/167 ,  C07H23/00

    Abstract: NEW MATERIAL:A compound of formula I [B is nucleic acid base which may be protected; R, R' are phenyl, branched alkyl or 3 or more carbon atoms.; R'' is tetrahydropyranyl residue]. EXAMPLE:2'-O-tetrahydropyranyl-3',5'-O-(di-tert.-butylsilandiyl)-uridine. USE:An intermediate of RNA-relating compounds in genetic engineering. PREPARATION:The nucleoside of formula II is silylated with a difunctional silicon compound of the formula: RR'SiX2 (X is acid residue), then allowed to react with dihydropyran.

    MANIFESTATION VECTOR PTP104-4
    33.
    发明专利
    MANIFESTATION VECTOR PTP104-4 失效

    公开(公告)号：JPH01144979A

    公开(公告)日：1989-06-07

    申请号：JP30215787

    申请日：1987-11-30

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： IWAKURA MASAHIRO ,  FURUSAWA KIYOTAKA ,  SAKAI TSUKASA ,  TANAKA YOSHIO

    IPC: C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/53 ,  C12N15/62 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

    Abstract: PURPOSE:To produce a substance containing a heterogenic product fused to a carboxy-terminal of dihydrofolic acid reductase(DHFR), in high efficiency, by modifying a plasmid pTP70-1 capable of easily utilizing a DHFR gene as a genetic marker. CONSTITUTION:A terminator region of rrnB gene (one of ribosomal RNA genes) known as an efficient terminator is introduced at the downstream side of a DHFR gene of plasmid pTP70-1 to obtain a plasmid pTP104-4 capable of efficiently producing a substance containing heterogenic product fused to the carboxy-terminal of DHFR. The plasmid has a size of 4,466 base pairs and can impart a host E.coli with trimethoprim resistance and ampicillin resistance.

    PRODUCING APPARATUS FOR PROTEIN THIN FILM
    35.
    发明专利
    PRODUCING APPARATUS FOR PROTEIN THIN FILM 失效

    公开(公告)号：JPS61291994A

    公开(公告)日：1986-12-22

    申请号：JP13498685

    申请日：1985-06-20

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： SORA TATSUO ,  SAKAI TSUKASA ,  TSUDA KEISHIRO

    IPC: C25D13/00 ,  C25D13/04 ,  C25D13/08 ,  G01N27/30 ,  G01N27/327

    Abstract: PURPOSE:To produce a uniform protein thin film free from the impurities on an electrode quickly and easily by dropping a small quantity of liquid contg. a charged colloidal particle on the electrode and bringing the other electrode into contact with the liquid from an upper part to perform the electrodeposition. CONSTITUTION:A movable arm 4 capable of regulating a position in the vertical direction is fitted to a vertical axis 2 fixed on a horizontal base 1 with a knob 3. A micrometer head 5 fitted with a metallic electrode 6 such as Pt used in case of the electrodeposition is fixed t the arm 4. On the other hand, a stage 8 of an electrode holder 9 mounted with a metallic electrode 10 is horizontally transferred on the above-mentioned horizontal base 1 by operating a knob 7 and the above-mentioned electrode 10 is properly positioned. Then after dropping a small quantity of a liquid 11 contg. a high molecular electrolyte such as protein and a charged colloidal particle such as a biomembrane fraction, the above-mentioned electrode 6 is descended by operating the knob 3 and brought into contact with the above-mentioned liquid 11. Thereafter both electrodes 6, 10 are joined to a DC electric power source 12 to perform the electrodeposition and a protein thin film is formed on the electrode 10.

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