公开(公告)号：CA3095749A1

公开(公告)日：2019-10-10

申请号：CA3095749

申请日：2019-04-02

Applicant: HARVARD COLLEGE

Inventor： NOVAK RICHARD ,  JALILI-FIROOZINEZHAD SASAN ,  GAZZANIGA FRANCESCA S ,  CALAMARI ELIZABETH L ,  CAMACHO DIOGO M ,  NESTOR BRET A ,  FADEL CICELY ,  CRONCE MICHAEL L ,  KASPER DENNIS L ,  INGBER DONALD E ,  BEIN AMIR

IPC: C12M1/12 ,  C12M3/06 ,  G01N33/48 ,  G01N33/50

Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.

公开(公告)号：AU2017326179A1

公开(公告)日：2019-03-28

申请号：AU2017326179

申请日：2017-09-13

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  KASENDRA MAGDALENA ,  SONTHEIMER-PHELPS ALEXANDRA ,  TOVAGLIERI ALESSIO

IPC: C12N5/071 ,  A61L27/36 ,  C12N5/078 ,  C12N5/28 ,  C12Q1/18

Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.

公开(公告)号：AU2017202616B2

公开(公告)日：2019-01-03

申请号：AU2017202616

申请日：2017-04-20

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  KIM HYUN JUNG

IPC: C12M3/02 ,  C12N1/14 ,  C12N1/20 ,  C12N5/071

Abstract: Abstract The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

公开(公告)号：AU2018204114A1

公开(公告)日：2018-06-28

申请号：AU2018204114

申请日：2018-06-08

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  SUPER MICHAEL ,  WATTERS ALEXANDER L ,  SNELL PHILIP T

IPC: C07K14/805 ,  A61K38/00

Abstract: Described herein are heme-binding compositions and methods relating to their use, e.g. methods of treatment of, for example, sepsis and rhabdomyolysis.

公开(公告)号：CA3031439A1

公开(公告)日：2018-01-25

申请号：CA3031439

申请日：2017-07-18

Applicant: HARVARD COLLEGE

Inventor： GOYAL GIRIJA ,  INGBER DONALD E

IPC: A61K35/28 ,  A61P7/00 ,  A61P35/00 ,  C12N5/07

Abstract: An organ-on-a-chip microfluidic device is disclosed that mimics a human lymph node and/or human lymphoid tissue. The device can include cells from human blood and lymphatic tissue, include an extracellular matrix for the development of immune system components, and provide for the perfusion of fluids and solids resembling blood and lymphatic fluid within micrometer sized channels.

公开(公告)号：AU2016200506B2

公开(公告)日：2017-11-02

申请号：AU2016200506

申请日：2016-01-28

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  KORIN NETANEL ,  KANAPATHIPILLAI MATHUMAI

IPC: A61K47/00 ,  A61K31/00 ,  A61P9/00

Abstract: The invention provides compositions and methods for treating or imaging stenosis, stenotic lesions, occluded lumens, O embolic phenomena or throibotic disorders. The invention further provides compositions and methods for treating internal hemor rhage.

公开(公告)号：AU2012284097B2

公开(公告)日：2017-08-03

申请号：AU2012284097

申请日：2012-07-18

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  SUPER MICHAEL ,  WAY JEFFREY CHARLES ,  CARTWRIGHT MARK J ,  WATTERS ALEXANDER ,  BERTHET JULIA B ,  SUPER DINAH R ,  ROTTMAN MARTIN M

IPC: C07K14/47 ,  C07K14/705 ,  G01N33/543 ,  G01N33/569

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：CA3013309A1

公开(公告)日：2017-06-08

申请号：CA3013309

申请日：2016-12-02

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  HAMILTON GERALDINE ,  JANG KYUNG-JIN ,  HANEY SUZZETTE ,  PATEL PAYAL ,  HERLAND ANNA ,  RESNIKOFF JOSHUA

IPC: C12M3/00 ,  B01L99/00 ,  B81B7/00 ,  C12N5/22 ,  G01N33/483 ,  G01N33/50

Abstract: Provided herein relates to devices for simulating a function of a tissue and methods of using the same. In some embodiments, the devices can be used to simulate a function of a human liver tissue. In some embodiments, the devices can be used to simulate a function of a dog liver tissue. Endothelial cell culture media for long-term culture of endothelial cells are also described herein.

公开(公告)号：AU2015289999A2

公开(公告)日：2017-03-16

申请号：AU2015289999

申请日：2015-07-10

Applicant: HARVARD COLLEGE

Inventor： HINOJOSA CHRISTOPHER DAVID ,  SLIZ JOSIAH ,  THOMPSON GUY ,  GEISLER HUBERT ,  FERNANDEZ-ALCON JOSE ,  INGBER DONALD E ,  LEVNER DANIEL

IPC: B82Y15/00 ,  C12M1/00 ,  C12Q1/02

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

公开(公告)号：CA2983821A1

公开(公告)日：2017-01-05

申请号：CA2983821

申请日：2016-04-25

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  VAN DER MEER ANDRIES ,  HERLAND ANNA

IPC: C12M1/02

Abstract: Systems and methods for producing and using a body having a first structure defining a first chamber, a second structure defining a second chamber, a membrane located at an interface region between the first chamber and the second chamber to separate the first chamber from the second chamber. The first chamber comprises a first permeable matrix disposed therein and the first permeable matrix comprises at least one or a plurality of lumens each extending therethrough, which is optionally lined with at least one layer of cells. The second chamber can comprise cells cultured therein. The systems and methods described herein can be used for various applications, including, e.g., growth and/or differentiation of primary cells, and/or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents). The systems and methods can also permit co-cultures of two or more different cell types.