公开(公告)号：DE60225157T2

公开(公告)日：2009-03-05

申请号：DE60225157

申请日：2002-09-05

Applicant: INTEL CORP

Inventor： RAO VALLURI ,  NEUBAUER GABI ,  KIRCH STEVEN ,  YAMAKAWA MINEO ,  BERLIN ANDREW

IPC: C12Q1/68 ,  B01L3/00 ,  C07H21/02 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N15/00 ,  C12N15/09 ,  C12P19/34 ,  G01J3/42 ,  G01J3/427 ,  G01J3/44 ,  G01N21/65 ,  G01N33/53 ,  G01N33/566

Abstract: The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.

公开(公告)号：GB2427469B

公开(公告)日：2008-10-29

申请号：GB0614242

申请日：2004-12-28

Applicant: INTEL CORP

Inventor： SU XING ,  ZHANG JINGWU ,  SUN LEI ,  BERLIN ANDREW

IPC: G01N33/543 ,  G01N21/65

Abstract: Composite organic-inorganic nanoclusters (COINs) are provided that produce surface-enhanced Raman signals (SERS) when excited by a laser. The nanoclusters include metal particles and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanocluster and a wide variety of Raman-active organic compounds and combinations thereof can be incorporated into the nanocluster. In addition, polymeric microspheres containing the nanoclusters and methods of making them are also provided. The nanoclusters and microspheres can be used, for example, in assays for multiplex detection of biological molecules.

公开(公告)号：DE60316150D1

公开(公告)日：2007-10-18

申请号：DE60316150

申请日：2003-05-16

Applicant: INTEL CORP

Inventor： CHAN SELENA ,  BERLIN ANDREW ,  MINEO YAMAKAWA

IPC: G01J3/44 ,  C12Q1/68 ,  G01N21/65 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553

Abstract: The methods and apparatus 300 disclosed herein concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates 240, 340 . In certain embodiments of the invention, porous silicon substrates 110, 210 may be formed by anodic etching in dilute hydrofluoric acid 150 . A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon 110, 210 by cathodic electromigration or any known technique. The metal-coated substrate 240, 340 provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain embodiments of the invention, metal nanoparticles may be added to the metal-coated substrate 240, 340 to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus 300.

公开(公告)号：DE60309903D1

公开(公告)日：2007-01-04

申请号：DE60309903

申请日：2003-09-24

Applicant: INTEL CORP

Inventor： SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  YAMAKAWA MINEO ,  BERLIN ANDREW

IPC: G01N33/543 ,  C12Q1/68 ,  G01B7/34

Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

公开(公告)号：AU2003303106A1

公开(公告)日：2004-08-13

申请号：AU2003303106

申请日：2003-11-26

Applicant: INTEL CORP

Inventor： RAO VALLURI ,  YAMAKAWA MINEO ,  BERLIN ANDREW

IPC: B01J19/00 ,  B05D3/00 ,  C12M1/34 ,  C12Q1/68 ,  G03F1/00 ,  G03F7/20

Abstract: A method and apparatus for forming a polymer array on a substrate suitable for synthesizing polymer sequences. This includes forming an array, each location of the array having at least one strand end, forming photosensitive protection on the strand ends, and selectively scanning and modulating at least one energy beam to expose a pattern on the photosensitive protection. In some embodiments, the method further includes removing a protective group from selected strand ends based on the exposed pattern. The method then includes adding a predetermined one or more polymeric subunits to the deprotected strand ends. In some embodiments the photosensitive protection includes a layer of photoresist to cover the strand ends. Some embodiments use an ultra-violet laser.

公开(公告)号：AU2003272286A1

公开(公告)日：2004-04-08

申请号：AU2003272286

申请日：2003-09-05

Applicant: INTEL CORP

Inventor： BERLIN ANDREW ,  YAMAKAWA MINEO ,  CHAN SELENA ,  SUNDARARAJAN NARAYANAN

IPC: G01N37/00 ,  B01D61/02 ,  B01D61/14 ,  B01D61/18 ,  B01D61/20 ,  B01D69/02 ,  B01D71/02 ,  B01J19/00 ,  B01L3/00 ,  B81B1/00

公开(公告)号：AU2002332897A1

公开(公告)日：2003-04-07

申请号：AU2002332897

申请日：2002-09-05

Applicant: INTEL CORP

Inventor： NEUBAUER GABI ,  YAMAKAWA MINEO ,  BERLIN ANDREW ,  KIRCH STEVEN ,  RAO VALLURI

IPC: B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  G01J3/44 ,  G01N21/65 ,  G01N33/53 ,  G01N33/566 ,  C07H21/02 ,  G01J3/427 ,  C07H21/04 ,  G01J3/42 ,  C12P19/34

Abstract: The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.

公开(公告)号：DE112005001895B4

公开(公告)日：2011-12-08

申请号：DE112005001895

申请日：2005-07-20

Applicant: INTEL CORP

Inventor： KOO TAE-WOONG ,  BERLIN ANDREW ,  SALSMAN KEN ,  OSTROVSKY BRIAN

IPC: C12Q1/68 ,  G01N21/35

Abstract: Es werden Verfahren und Systeme zum Detektieren biomolekularer Bindungsereignisse mittels Gigahertz- und Terahertzstrahlung bereitgestellt. Die Verfahren und Systeme verwenden Niedrig-Energie-Spektroskopie zum Detektieren biomolekularer Bindungsereignisse zwischen Molekülen in einer wäßrigen Lösung. Die detektierten biomolekularen Bindungsereignisse umfassen beispielsweise Nukleinsäurehybridisierung, Antikörper-/Antigenbindung und Rezeptor-/Ligandbindung.

公开(公告)号：AT394680T

公开(公告)日：2008-05-15

申请号：AT03819075

申请日：2003-10-31

Applicant: INTEL CORP

Inventor： SU XING ,  BERLIN ANDREW

IPC: G01N33/68 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  G01N33/66 ,  G06F19/00

Abstract: The methods and apparatus, disclosed herein are of use for sequencing and/or identifying proteins, polypeptides and/or peptides. Proteins containing labeled amino acid residues may be synthesized and passed through nanopores. A detector operably coupled to a nanopore may detect labeled amino acid residues as they pass through the nanopore. Distance maps for each type of labeled amino acid residue may be compiled. The distance maps may be used to sequence and/or identify the protein. Apparatus of use for protein sequencing and/or identification is also disclosed herein. In alternative methods, other types of analytes may be analyzed by the same techniques.

公开(公告)号：AT386822T

公开(公告)日：2008-03-15

申请号：AT02799571

申请日：2002-09-05

Applicant: INTEL CORP

Inventor： RAO VALLURI ,  NEUBAUER GABI ,  KIRCH STEVEN ,  YAMAKAWA MINEO ,  BERLIN ANDREW

IPC: C12Q1/68 ,  B01L3/00 ,  C07H21/02 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N15/00 ,  C12N15/09 ,  C12P19/34 ,  G01J3/42 ,  G01J3/427 ,  G01J3/44 ,  G01N21/65 ,  G01N33/53 ,  G01N33/566

Abstract: The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.