公开(公告)号：GB2363026A

公开(公告)日：2001-12-05

申请号：GB0108719

申请日：2001-04-06

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  HOFFMANN JUERGEN ,  GUGEL HILMAR ,  BEWERSDORF JOERG

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/06

Abstract: A double confocal scanning microscope (1) has an illuminating beam path (2) associated with a light source (3) and a detected beam path (4) associated with a detector (5). In order to achieve a resolution capability approaching that theoretically possible, in particular in the context of multi-color fluorescence applications, the optical properties of components (6, 10, 13, 14) arranged in the beam path are co-ordinated with one another in such a way that the accumulated aberrations, with respect to the optical axis (33) and/or at least one surface (18, 19, 20) in a specimen support region, are at least of the order of magnitude of the theoretically achievable resolution capability.

公开(公告)号：DE10018256A1

公开(公告)日：2001-10-25

申请号：DE10018256

申请日：2000-04-13

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  BEWERSDORF JOERG ,  GUGEL HILMAR ,  HOFFMANN JUERGEN

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/06

Abstract: The present invention concerns a double confocal scanning microscope ( 1 ) having an illuminating beam path ( 2 ) of at least one light source ( 3 ), and a detected beam path ( 4 ) of at least one detector ( 5 ), and in order to achieve almost the theoretically possible resolution capability, in particular in the context of multi-color fluorescence applications, is characterized in that the optical properties in particular of the components ( 6, 10, 13, 14 ) arranged in the beam path are coordinated with one another in such a way that the accumulated aberrations, with respect to the optical axis ( 33 ) and/or at least one surface ( 18, 19, 20 ) in the specimen region, are at least of the order of magnitude of the theoretically achievable resolution capability.