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    33.
    发明专利
    未知

    公开(公告)号：DE102005013116A1

    公开(公告)日：2006-10-05

    申请号：DE102005013116

    申请日：2005-03-18

    Applicant: LEICA MICROSYSTEMS

    Inventor： GUGEL HILMAR ,  SEYFRIED VOLKER

    IPC: G02B5/30 ,  G02B21/06 ,  G02B27/50

    Abstract: The invention relates to a phase filter (1) for a light beam in a microscope, particularly for STED microscopy, which has at least one first section (18) and at least one second section (19) that is complementary to the at least one first section (18). Light interacting with the at least one first section (18) and light interacting with the at least second section (19) have, after the interaction, phases that differ by 180°. The phase filter is designed and improved with regard to a particularly high resolution of a microscope operated with the phase filter (1). To this end, the sections (18, 19) are arranged relative to one another so that they are axially symmetric in a complementary manner along a first axis (20), whereby the symmetry of the at least one first section (18) transitions into its complementary at least one second section (19), and that they are mirror symmetric along an axis (21) perpendicular to the first axis (20), or that the sections (18, 19) are arranged relative to one another so that they are rotationally symmetric in a complementary manner with regard to a rotation of 360°/(2*n) with n>2 about the axis of rotation (22), whereby a rotation of this type causes the at least one first section (18) to transition into its complementary at least one second section (19).

    34.
    发明专利
    未知

    公开(公告)号：DE10347712A1

    公开(公告)日：2005-05-12

    申请号：DE10347712

    申请日：2003-10-14

    Applicant: LEICA MICROSYSTEMS

    Inventor： GUGEL HILMAR

    IPC: G02B21/00 ,  G02B21/06 ,  G02B21/16

    Abstract: In a scanning microscope that impinges upon a sample with a first light pulse and a second light pulse, a dispersive medium that modifies the time offset between the first and the second light pulse is provided in the beam path of at least one of the light pulses.

    38.
    发明专利
    未知

    公开(公告)号：DE102006009833B4

    公开(公告)日：2009-01-08

    申请号：DE102006009833

    申请日：2006-03-01

    Applicant: LEICA MICROSYSTEMS

    Inventor： DYBA MARCUS ,  GUGEL HILMAR

    IPC: G01N21/64 ,  G02B21/00

    Abstract: The process involves bringing a substance of a sample in a sample region to be detected in a condition. Another condition is induced by optical signals, and spatial limited partial regions within the sample region to be detected are omitted in a selective manner. The optical signal is provided such that a vertical wave (9) with defined intensity-zero is formed in the sample region to be detected, where the vertical wave is generated by focusing coherent light rays (10,11) in a pupil of an objective (13). The coherent light rays are provided by using glass fibers. An independent claim is also included for a microscope, in particular laser-raster-fluorescence microscope for spatial high resolution examination of samples.

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