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    SUBSTITUTED PHENYLACETIC ACIDS AND SALTS AS TBP BLOCKING AGENTS IN IODOTHYRONINE IMMUNOASSAYS
    33.
    发明专利
    SUBSTITUTED PHENYLACETIC ACIDS AND SALTS AS TBP BLOCKING AGENTS IN IODOTHYRONINE IMMUNOASSAYS 未知

    公开(公告)号：CA1208547A

    公开(公告)日：1986-07-29

    申请号：CA414812

    申请日：1982-11-03

    Applicant: MILES LAB

    Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

    IPC: G01N33/78 ,  G01N33/566 ,  G01N33/573

    Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

    APOGLUCOSE OXIDASE PREPARATION
    37.
    发明专利
    APOGLUCOSE OXIDASE PREPARATION 未知

    公开(公告)号：CA1143309A

    公开(公告)日：1983-03-22

    申请号：CA351831

    申请日：1980-05-13

    Applicant: MILES LAB

    Inventor： ELLIS PAUL B ,  MORRIS DAVID L

    IPC: C12Q1/26 ,  C12N9/04 ,  G01N33/542

    Abstract: An apoglucose oxidase preparation having a residual glucose oxidase activity of less than 0.05?, and preferably less than 0.005?. The preparation is obtained by incubation of glucose oxidase in aqueous solution at a pH of less than about 2 and in the presence of about 20 to about 40, preferably about 30, percent by volume glycerol, and separating resulting dissociated prosthetic group, flavin adenine dinucleotide, from apoglucose oxidase by column chromatography, preferably on cross-linked dextran gel. The purified apoglucose oxidase is useful as a reagent in specific binding assays employing flavin adenine dinucleotide as a label.

    SPECIFIC BINDING ASSAY WITH A PROSTHETIC GROUP AS A LABEL COMPONENT
    38.
    发明专利
    SPECIFIC BINDING ASSAY WITH A PROSTHETIC GROUP AS A LABEL COMPONENT 未知

    公开(公告)号：CA1117415A

    公开(公告)日：1982-02-02

    申请号：CA330233

    申请日：1979-06-21

    Applicant: MILES LAB

    Inventor： HORNBY WILLIAM E ,  MORRIS DAVID L

    IPC: C12N9/96 ,  G01N33/542 ,  G01N33/78 ,  C12Q1/26

    Abstract: A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate. Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising s?ch prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after thc binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group-labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

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