公开(公告)号：FR2540516B1

公开(公告)日：1987-08-07

申请号：FR8401863

申请日：1984-02-07

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO

IPC: C12N15/09 ,  A61K39/05 ,  C07K14/34 ,  C12N15/00 ,  C12N15/12 ,  C12P21/00 ,  C12R1/16 ,  C12P21/02

Abstract: Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 mu g/ml to 0.5 mu g/ml, at a temperature of from 30 DEG C. to 40 DEG C., in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.

公开(公告)号：CA1212642A

公开(公告)日：1986-10-14

申请号：CA446410

申请日：1984-01-31

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO

IPC: C12N15/09 ,  A61K39/05 ,  C07K14/34 ,  C12N15/00 ,  C12N15/12 ,  C12P21/00 ,  C12R1/16

Abstract: PROCESS FOR PRODUCING PROTEINS CORRELATED WITH THE DIPHTHERIC TOXIN Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 .mu.g/ml to 0.5 .mu.g/ml, at a temperature of from 30.degree.C to 40.degree.C, in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.

公开(公告)号：AT176913T

公开(公告)日：1999-03-15

申请号：AT89912578

申请日：1989-10-20

Applicant: UNIV LELAND STANFORD JUNIOR ,  SCLAVO SPA

Inventor： RELMAN DAVID A ,  DOMENIGHINI MARIO ,  RAPPUOLI RINO ,  FALKOW STANLEY

IPC: C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61K39/395 ,  A61P37/04 ,  C07K14/235 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19

公开(公告)号：DE69022106T2

公开(公告)日：1996-02-15

申请号：DE69022106

申请日：1990-04-25

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO ,  NENCIONI LUCIANO

IPC: C12N1/20 ,  A61K39/00 ,  A61K39/10 ,  C07K14/235 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/31 ,  C12R1/01

公开(公告)号：DK0396964T3

公开(公告)日：1995-10-30

申请号：DK90107892

申请日：1990-04-25

Applicant: SCLAVO SPA

Inventor： NENCIONI LUCIANO ,  PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO

IPC: C12N1/20 ,  A61K39/00 ,  A61K39/10 ,  C07K14/235 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/31 ,  C12R1/01

公开(公告)号：IT1248735B

公开(公告)日：1995-01-26

申请号：IT2071090

申请日：1990-06-21

Applicant: SCLAVO SPA

Inventor： NENCIONI LUCIANO ,  BUGNOLI MASSIMO ,  MANETTI ROBERTO ,  MARSILI ILIO ,  RUGGIERO PAOLO ,  RAPPUOLI RINO

IPC: A61K39/10 ,  A61K38/00 ,  A61K39/00 ,  A61K39/102 ,  A61K39/116 ,  C07K14/235 ,  C12N1/21 ,  C12N9/10 ,  C12N15/31 ,  C12R1/01 ,  A61K

公开(公告)号：DE3889562T2

公开(公告)日：1994-12-15

申请号：DE3889562

申请日：1988-03-01

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： VELATI BELLINI ADA ,  TOMA SALVATORE ,  GRANDI GUIDO ,  COLLETTI ELISABETTA ,  RIBOLI BARBARA ,  COSMINA PAOLA ,  TOGNONI ANGELO ,  PEDRONI PAOLA ,  RAPPUOLI RINO

IPC: A61K39/10 ,  A61K39/00 ,  A61K39/07 ,  C07K14/235 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/70 ,  C12N15/75 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/04 ,  C12R1/07 ,  C12R1/125 ,  C12R1/19 ,  G01N33/569 ,  G01N33/577

公开(公告)号：DE3750094D1

公开(公告)日：1994-07-28

申请号：DE3750094

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：DE3878553T2

公开(公告)日：1993-09-02

申请号：DE3878553

申请日：1988-12-15

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： PEDRONI PAOLA ,  RIBOLI BARBARA ,  DE FERRA FRANCESCA ,  GRANDI GUIDO ,  TOMA SALVATORE ,  ARICO BEATRICE ,  RAPPUOLI RINO

IPC: C12N1/20 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  A61P37/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/19 ,  C12N1/21 ,  C12N15/30 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19

Abstract: The cloning and sequencing of the gene which codes for a new pilinic subunit of Bordetella pertussis are described. The aminoacid sequence of the mature subunit, deduced from its necleotide sequence, is similar but not identical to that of the known pilins 2, 3 and 6. Polypeptides having the aminoacid sequence of the mature pilinic subunit or of regions thereof are particularly useful for the development of synthetic acellular vaccines against pertussis.

公开(公告)号：CA2045071A1

公开(公告)日：1991-12-22

申请号：CA2045071

申请日：1991-06-20

Applicant: SCLAVO SPA

Inventor： NENCIONI LUCIANO ,  BUGNOLI MASSIMO ,  MANETTI ROBERTO ,  MARSILI ILIO ,  RUGGIERO PAOLO ,  RAPPUOLI RINO

IPC: A61K39/10 ,  A61K38/00 ,  A61K39/00 ,  A61K39/102 ,  A61K39/116 ,  C07K14/235 ,  C12N1/21 ,  C12N9/10 ,  C12N15/31 ,  C12R1/01

Abstract: Novel acellular anti-pertussis vaccines are described, which comprises a non-toxic double mutant of pertussis toxin (PT), filamentous haemagglutinin (FHA) and the 69 kilodaltons protein (69Kd) taken alone or in combination with the diphtheria and tetanus anatoxin. The completely non-toxic double mutant of the PT is characterized by the fact that is comprises in the amino acid sequence of the S1 subunit the substitution of Glu 129 and of one of any other amino acid of the subunit, whose substitution brings about a decrease in toxicity, by natural amino acids. Moreover there are described purification methods for, and the characterisation of the FHA and the 69 Kd protein expressed by transformed B. pertussis W28-9K/129G strains, which produce the PT double mutant and by transformed B. pertussis W28- Tox which do not produce the PT. These proteins are particularly suitable for preparing safer and more immunogenic acellular anti-pertussis vaccine.