公开(公告)号：US4628026A

公开(公告)日：1986-12-09

申请号：US552090

申请日：1983-11-15

Applicant: Dietlind Gardell ,  Gail Rock

Inventor： Dietlind Gardell ,  Gail Rock

IPC: G01N21/25 ,  G01N21/64 ,  G01N33/53 ,  G01N33/554

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/0446 ,  G01N2201/0484 ,  Y10S435/963 ,  Y10S435/966 ,  Y10S435/973 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/821

Abstract: An automated system for rapid sequential photometric analysis of a collection of double fluorochrome stained lymphocyte specimens, useful for antibody screening or lymphocytotoxicity analysis. The specimens are sequentially alternately irradiated with light of two distinguishable wavelengths, producing fluorescence at two distinguishable wavelengths. The fluorescent emission light intensity for each irradiation of each specimen is measured using a photometer and computer. The computer controls the synchronization of the irradiation through alternately selected condenser sets with the sequential movement of specimens into the optical path of the irradiating and detected light, and calculates the quotient of the light intensities emitted from each specimen at the two selected fluorescent light wavelengths. These quotients are compared against a control ratio (for lymphocytotoxicity analysis) to classify the specimen. Also described is a method of preparing specimens for such analysis, which requires that a complement be added to the first staining solution after the latter is applied to the specimens, then this combination agitated, and then the second staining solution added and the specimen incubated.

Abstract translation: 一种用于快速顺序光度分析双重荧光染色淋巴细胞标本的自动化系统，可用于抗体筛选或淋巴细胞毒性分析。 用两个可辨别的波长的光顺序地交替地照射样品，在两个可区别的波长处产生荧光。 使用光度计和计算机测量每个样品的每次照射的荧光发射光强度。 计算机通过交替选择的聚光器组来控制照射的同步，同时将样品顺序移动到照射和检测光的光路中，并计算在两个选定的荧光波长处从每个样品发射的光强度的商。 将这些商与对照比（用于淋巴细胞毒性分析）进行比较以对样品进行分类。 还描述了制备用于这种分析的样品的方法，其要求在将样品应用于样品后将补体添加到第一染色溶液中，然后将该组合搅拌，然后加入第二染色溶液并将样品孵育。

公开(公告)号：WO2014016500A1

公开(公告)日：2014-01-30

申请号：PCT/FR2013/051740

申请日：2013-07-18

Applicant: MSC & SGCC

Inventor： COLLE, Olivier ,  DROUET, Florence ,  LECONTE, Marc

IPC: G01N21/90 ,  G01N21/88

CPC classification number: G01N21/90 ,  G01N21/8851 ,  G01N21/958 ,  G01N2021/8832 ,  G01N2201/0484 ,  G01N2201/062

Abstract: L'invention concerne un procédé optique d'inspection en ligne de récipients transparents ou translucides (3) défilant entre une source lumineuse (7) et un système (9) de prise d'images des récipients et d'analyse des images prise. Selon se procédé; on illumine chaque récipient (3) par la source lumineuse (7) présentant une variation d'intensité lumineuse selon un motif périodique (7 1 ) de période T1 selon au moins, une première direction de variation (D), on prend pour chaque récipient (3), un nombre N supérieur ou égal à trois, d'images du récipient défilant devant la source lumineuse et occupant respectivement N positions différentes le long de la trajectoire de défilement, entre chaque prise d' image, on crée un décalage relatif entre le récipient et le motif périodique selon une direction de variation (D) du motif périodique (7 1 ), » on détermine et applique une transformation géométrique, dans au moins N-1 images d'un même récipient, pour au moins un ensemble de points appartenant au récipient, afin de mettre en coïncidence les pixels appartenant au récipient dans les H images successives d'un même récipient on construit pour chaque récipient (3), à partir des N images recalées du récipient, une image de phase, on analyse limage de phase afin d'en déduire au moins la présence d'un défaut réfractant la lumière ou la qualité de répartition du matériau constitutif du récipient.

Abstract translation: 本发明涉及一种在线光学方法，用于检查在光源（7）和系统（9）之间行进的透明或半透明容器（3），用于拍摄容器的图像并分析所拍摄的图像。 根据本发明，该方法包括以下步骤：使用具有根据周期T1周期性图案（71）的光强度变化的光源（7）沿着至少一个第一变化方向照亮每个容器（3） D）; 对于每个容器（3），取经过光源前面的容器的数量大于或等于3的图像N，沿着路径的长度分别占据N个不同位置; 在每个图像捕获之间，根据周期性图案（71）的变化方向（D）产生容器和周期性图案之间的相对移动; 确定并应用属于容器的至少一组点的单个容器的至少N-1个图像中的几何变换，以便将属于容器的像素对准单个容器的N个连续图像; 对于每个容器（3），从容器的N个调整图像构造相位图像; 并且分析相位图像以便至少推导出光折射缺陷的存在或形成容器的材料的分布质量。

公开(公告)号：WO2013186672A2

公开(公告)日：2013-12-19

申请号：PCT/IB2013/054660

申请日：2013-06-06

Applicant: BIO-RAD LABORATORIES INC.

Inventor： URI, Yochanan ,  RAN, Boaz ,  NOTCOVICH, Ariel G. ,  NIMRI, Shay

IPC: G01N21/03 ,  G01N21/55

CPC classification number: G01N21/553 ,  G01N21/253 ,  G01N21/658 ,  G01N33/54373 ,  G01N2021/0328 ,  G01N2201/0407 ,  G01N2201/0484 ,  G01N2201/062

Abstract: A tip for use in an optical detection system to analyze an analyte in a fluid sample drawn into the tip, using light reflected from a detection surface inside the tip that the analyte binds to, comprising a first detection surface and a second detection surface located in a same flow path with no controllable valve separating them, wherein the first and second detection surfaces have different surface chemistries.

Abstract translation: 一种用于光学检测系统的尖端，用于使用从分析物结合到所述尖端内部的检测表面反射的光来分析吸入所述尖端的流体样品中的分析物，所述检测表面包括第一检测表面和位于 相同的流动路径，没有可分离它们的可控阀，其中第一和第二检测表面具有不同的表面化学性质。

公开(公告)号：US20240302359A1

公开(公告)日：2024-09-12

申请号：US18666008

申请日：2024-05-16

Applicant: Meso Scale Technologies, LLC.

Inventor： Jeff Debad

IPC: G01N33/53 ,  G01N21/66 ,  G01N35/02

CPC classification number: G01N33/5308 ,  G01N21/66 ,  G01N35/02 ,  G01N2201/0484 ,  G01N2800/28

Abstract: The present invention relates to assays, methods, and kits to assess traumatic brain injury (TBI) in a subject. The present invention also relates to ultrasensitive assays for GFAP, tau, CKBB, IL-1β, IL-2, IL-6, IL-10, IL-22, IP-10, TNFα, TSLP, NFL, and/or NFH.

公开(公告)号：US20230288331A1

公开(公告)日：2023-09-14

申请号：US18180228

申请日：2023-03-08

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Yunlei ZHAO ,  Guangzhong MA

IPC: G01N21/51 ,  B82Y15/00

CPC classification number: G01N21/51 ,  B82Y15/00 ,  G01N2201/0461 ,  G01N2201/0484 ,  G01N2201/067

Abstract: Provided herein are methods of determining molecular binding kinetics on particles, such as magnetic nanoparticles. In some embodiments, the methods include introducing an incident light from a light source toward a sample container that comprises a particle-bound biomolecule-ligand composition comprising a plurality of particle-bound biomolecules and a plurality of ligands that binds, or is capable of binding, to biomolecules of the plurality of particle-bound biomolecules, detecting light scattered from particle-bound biomolecule-ligand complexes in the particle-bound biomolecule-ligand composition over a duration to produce a set of imaging data using the detector, and determining size or volume changes of one or more of the particle-bound biomolecule-ligand complexes during at least a portion of the duration from the set of imaging data to thereby determine the molecular binding kinetics on the particles. Related systems and computer readable media are also provided.

公开(公告)号：US20230266236A1

公开(公告)日：2023-08-24

申请号：US18173035

申请日：2023-02-22

Applicant: Hyperspectral Corp.

Inventor： Euan Mowat ,  Matthew Theurer ,  Sarah Rachel Hernandez ,  Mario Martinez

IPC: G01N21/31 ,  G01N21/27

CPC classification number: G01N21/314 ,  G01N21/274 ,  G01N2021/317 ,  G01N2201/12784 ,  G01N2201/0484 ,  G01N2201/061 ,  G01N2021/0125

Abstract: An example system includes a light intensity measuring apparatus couplable to a food processing apparatus and a computing system. The light intensity measuring apparatus includes a chamber configured to receive a water sample from the food processing apparatus, a light source, a detector configured to detect light that has passed through the water sample and measure multiple times intensities of wavelengths of the light to obtain multiple sets of measured intensities of wavelengths, and a communication module configured to provide the multiple sets of measured intensities of wavelengths. The computing system may receive the multiple sets of measured intensities, process the multiple sets to obtain a set of values, apply a first set of decision trees to the set of values to obtain a first result indicating a positive or negative foodborne pathogen detection, generate a notification indicating either the positive of negative detection, and provide the notification.

公开(公告)号：US20060006237A1

公开(公告)日：2006-01-12

申请号：US11174296

申请日：2005-07-01

Applicant: Debra Sillman ,  Xiangyang Zhou ,  Chetan Jadhaw ,  Herbert Cattell ,  Jayati Ghosh

Inventor： Debra Sillman ,  Xiangyang Zhou ,  Chetan Jadhaw ,  Herbert Cattell ,  Jayati Ghosh

IPC: G06F17/00

CPC classification number: G01N21/6452 ,  G01N33/52 ,  G01N33/582 ,  G01N33/68 ,  G01N2201/0484

Abstract: Methods for programming an array scanner to scan a plurality of biopolymer arrays are provided. In the subject methods, individual scanning parameters for at least two different arrays of the plurality of arrays to be scanned are selected and input into the array scanner prior to scanning the plurality arrays. The programmed scanner then scans the plurality of arrays according to the pre-selected scanning parameters Also provided are scanners programmed according to the subject methods, as well as computer programming that provides for programming of scanners according to the subject methods. The subject methods and compositions find use in a variety of different applications, including both genomics and proteomics applications.

Abstract translation: 提供了对阵列扫描器进行编程以扫描多个生物聚合物阵列的方法。 在主题方法中，在扫描多个阵列之前，选择要扫描的多个阵列的至少两个不同阵列的各个扫描参数并将其输入阵列扫描器。 编程的扫描仪然后根据预先选择的扫描参数扫描多个阵列。还提供了根据主题方法编程的扫描器以及根据主题方法提供对扫描仪的编程的计算机编程。 主题方法和组合物可用于各种不同的应用，包括基因组学和蛋白质组学应用。

公开(公告)号：US06913200B2

公开(公告)日：2005-07-05

申请号：US10301471

申请日：2002-11-20

Applicant: Debra Ann Sillman ,  Xiangyang Zhou ,  Chetan D Jadhaw ,  Herbert F. Cattell ,  Jayati Ghosh

Inventor： Debra Ann Sillman ,  Xiangyang Zhou ,  Chetan D Jadhaw ,  Herbert F. Cattell ,  Jayati Ghosh

IPC: G01N21/64 ,  G01N33/52 ,  G01N33/58 ,  G01N33/68 ,  G06F17/00 ,  G06F7/10 ,  G06F19/00

CPC classification number: G01N21/6452 ,  G01N33/52 ,  G01N33/582 ,  G01N33/68 ,  G01N2201/0484

Abstract: Methods for programming an array scanner to scan a plurality of biopolymer arrays are provided. In the subject methods, individual scanning parameters for at least two different arrays of the plurality of arrays to be scanned are selected and input into the array scanner prior to scanning the plurality arrays. The programmed scanner then scans the plurality of arrays according to the pre-selected scanning parametersAlso provided are scanners programmed according to the subject methods, as well as computer programming that provides for programming of scanners according to the subject methods. The subject methods and compositions find use in a variety of different applications, including both genomics and proteomics applications.

Abstract translation: 提供了对阵列扫描器进行编程以扫描多个生物聚合物阵列的方法。 在主题方法中，在扫描多个阵列之前，选择要扫描的多个阵列的至少两个不同阵列的各个扫描参数并将其输入阵列扫描器。 编程的扫描仪然后根据预先选择的扫描参数扫描多个阵列。还提供根据主题方法编程的扫描仪，以及根据主题方法提供对扫描仪的编程的计算机编程。 主题方法和组合物可用于各种不同的应用，包括基因组学和蛋白质组学应用。

公开(公告)号：US20050014281A1

公开(公告)日：2005-01-20

申请号：US10492899

申请日：2002-10-10

Applicant: Pieter Langeveld ,  Karl Hemert Van ,  Johannes Hendrik Kerkhof ,  Jacobus Stark

Inventor： Pieter Langeveld ,  Karl Hemert Van ,  Johannes Hendrik Kerkhof ,  Jacobus Stark

IPC: C12Q1/18 ,  G01J3/46 ,  G01J3/52 ,  G01N21/86 ,  G01N31/22 ,  G01N33/04 ,  G01N33/49 ,  G01N33/493

CPC classification number: G01J3/52 ,  G01J3/46 ,  G01J3/462 ,  G01J2003/466 ,  G01N31/221 ,  G01N33/04 ,  G01N33/49 ,  G01N33/493 ,  G01N2201/0484 ,  G01N2201/102

Abstract: Detection arrangement and method for detecting presence of a residue in a sample by determining color values of the sample, associated with the L*a*b color model, where a value of a composite parameter Z is calculated as follows: Z=wL+waa+wbb where wL, wa, and wb are weighting factors having a value depending on said residue and said sample, and a determination is made whether or not said sample comprises more or less than a predetermined amount of said residue in dependence on said value of said composite parameter Z. In a preferred embodiment, the arrangement is used to detect antibiotic residues, e.g. penicillin-G, in food products, e.g. milk, or body fluids, e.g. blood, urine.

Abstract translation: 用于通过确定与L * a * b颜色模型相关联的样品的颜色值来检测样品中残留物的检测装置和方法，其中复合参数Z的值如下计算：Z = wL + waa + wbb其中wL，wa和wb是具有取决于所述残基和所述样品的值的加权因子，并且根据所述残基和所述样品的值是否确定所述样品是否包含大于或小于预定量的所述残基 所述复合参数Z。在优选实施方案中，该装置用于检测抗生素残留物，例如， 青霉素G，在食品中，例如 牛奶或体液，例如， 血，尿。

公开(公告)号：US4710874A

公开(公告)日：1987-12-01

申请号：US743596

申请日：1985-06-11

Applicant: Paul-Henri Cinqualbre

Inventor： Paul-Henri Cinqualbre

IPC: G01N15/05 ,  G01N33/48 ,  G01N21/51 ,  G06F15/42

CPC classification number: G01N15/05 ,  G01N2201/0484

Abstract: An apparatus and a method for continuous display of a particle sedimentation rate in a liquid includes a rotary support device adapted to hold a plurality of light-transparent containers, and wherein each container holds a liquid. Any container may be selected to be observed so that at least a portion of the selected container may be observed from the exterior. A light source illuminates the selected container, and an image-forming device is arranged to form an image of the aforesaid portion of the selected container, and the liquid held therein. Video signals are created from the so-formed image, and a computer is arranged to process the video signals. A transmission conduit transmits the video signals from the image-forming device to the computer, and a display is provided for displaying the signals processed by the computer.

Abstract translation: 用于连续显示液体中的颗粒沉降速率的装置和方法包括适于容纳多个透光容器的旋转支撑装置，并且其中每个容器保持液体。 可以选择任何容器进行观察，使得可以从外部观察所选择的容器的至少一部分。 光源照亮所选择的容器，并且图像形成装置被布置成形成所选择的容器的上述部分的图像，并且保持在其中的液体。 视频信号是从如此形成的图像创建的，并且计算机被布置成处理视频信号。 传输导管将图像信号从图像形成装置发送到计算机，并且提供显示器以显示由计算机处理的信号。