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公开(公告)号：KR100482933B1
公开(公告)日：2005-04-14
申请号：KR1020020077531
申请日：2002-12-04
Applicant: 박근형
IPC: G01N33/14
Abstract: 본 발명은 진도홍주색소의 감별 방법에 관한 것으로, 지초를 사용하여 제조된 진도홍주를 분액여두를 이용하여 에칠아세테이트계 용매계로 분배하면 진도홍주의 홍색소가 이들 유기용매계로 분배되어지는 성질을 이용하여 감별하는 1단계 감별방법, 분광광도계의 의한 진도홍주색소의 분광학적 성질을 이용하여 감별하는 2단계 감별방법, 그리고 옥타데실실란 컬럼이 장착된 고성능액체크로마토그라피에 의해 진도홍주 홍색소 용출양상을 검토하여 감별하는 3단계 감별방법으로 구성된 진도홍주색소의 1, 2, 3단계 감별방법에 의해 진도홍주 홍색소가 지초유래의 색소인지를 간편하고도 정확하게 식별할 수 있는 감별방법을 제공한다.
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42.发明授权
公开(公告)号：KR100439012B1
公开(公告)日：2004-07-03
申请号：KR1020010017769
申请日：2001-03-29
Applicant: 박근형
Abstract: PURPOSE: A natural antioxidant substance containing 4-hydroxy-3-methoxy benzoic acid, 4-hydroxy-3-methoxy cinnamic acid, quercetin-3-0-β-D-glucopyranoside and hovenidine B6 as a new natural antioxidant active material separated from Hovenia dulcis Thunb. is provided which is expected to be used as a material of food and medicine having an anti-aging function. CONSTITUTION: Leaves and stems of Hovenia dulcis Thunb. are extracted in water, freeze dried and then extracted in methanol, followed by solvent fractionation to produce an ethyl acetate acidic fraction and neural fraction. An active material contained in the extract is then purified with a purifying means such as silica gel column chromatography, chromatography with Sephadex LH-20, octadecylsilane column chromatography, a reversed-phase high-performance liquid chromatography or the like.
Abstract translation: 用途：含有4-羟基-3-甲氧基苯甲酸，4-羟基-3-甲氧基肉桂酸，槲皮素-3-O-β-D-吡喃葡萄糖苷和hovenidine B6作为新的天然抗氧化剂活性物质的天然抗氧化剂物质 从Hovenia dulcis Thunb分离的材料。 预计将被用作具有抗衰老功能的食物和药物的材料。 组成：Hovenia dulcis Thunb的叶和茎。 在水中萃取，冷冻干燥，然后用甲醇萃取，随后溶剂分馏以产生乙酸乙酯酸性部分和神经部分。 然后提取物中所含的活性物质用硅胶柱色谱等纯化手段，Sephadex LH-20色谱法，十八烷基硅烷柱色谱法，反相高效液相色谱法等纯化。
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公开(公告)号：KR1020040048783A
公开(公告)日：2004-06-10
申请号：KR1020020077531
申请日：2002-12-04
Applicant: 박근형
IPC: G01N33/14
Abstract: PURPOSE: A discriminating method of Jindo-Hongju pigment is provided, thereby easily and accurately discriminating whether red pigment of Jindo-Hongju is derived from puccoon (Lithospermum erythrorhizon Sieb. et Zucc), and maintaining constant quality of Jindo-Hongju pigment. CONSTITUTION: The discriminating method of Jindo-Hongju pigment comprises the steps of: mixing Jindo-Hongju pigment with ethylacetate-hexane mixed solvent in a same ratio, or mixing Jindo-Hongju pigment diluted with 10 to 20% of water with ethyl acetate in a same ratio, settling the mixture for a certain time, and observing color of an upper organic layer with the naked eye; determining whether the Jindo-Hongju pigment has a peak with the highest optical density value at 520 nm, and peaks with second and third optical density values at 496 nm and 550 nm using a spectrophotometer; and measuring the elution pattern by subjecting the Jindo-Hongju pigment to HPLC using the octadecylsilane column.
Abstract translation: 目的：提供金道红菊色素的辨别方法，从而容易准确地区分金杜红色素是否源于茯苓（Lithospermum erythrorhizon Sieb。et Zucc），并保持金道红菊色素的质量。 构成：金道红菊颜料的鉴别方法包括以下方法：将金杜红色素与乙酸乙酯 - 己烷混合溶剂以相同的比例混合，或将乙酸乙酯稀释10〜20％的金杜红色素 相同比例，将混合物沉降一定时间，并用肉眼观察上部有机层的颜色; 确定Jindo-Hongju颜料是否具有在520nm处具有最高光密度值的峰值，并且使用分光光度计在496nm和550nm处具有第二和第三光密度值的峰值; 并使用十八烷基硅烷柱对金杜红菊颜料进行HPLC测定。
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44.发明授权3-메톡시-4-하이드록시벤조산,3-메톡시-4-하이드록시시나믹산, 4-하이드록시벤조산,3,4-디하이드록시벤조산, 4-하이드록시시나믹산 등을포함한 땅콩껍질을 이용한 천연보존제 失效3-메톡시-4-하이드록시벤조산，3-메톡시-4-하이드시시시믹산，4-하이드록시벤조산，3,4-하이드록시벤조산，4-하이드록시시산믹산포함한땅콩껍질을 使用说明
公开(公告)号：KR100424932B1
公开(公告)日：2004-03-30
申请号：KR1020010022481
申请日：2001-04-20
Applicant: 박근형
IPC: C07C51/42
Abstract: PURPOSE: Provided is a natural preservative using the skin of Arachis hypogaea containing natural antimicrobial and antioxidant active materials such as 3-methoxy-4-hydroxybenzoic acid, 3-methoxy-4-hydroxycinnamic acid, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 4-hydroxycinnamic acid, and etc. CONSTITUTION: The 3-methoxy-4-hydroxybenzoic acid, the 3-methoxy-4-hydroxycinnamic acid, the 4-hydroxybenzoic acid, the 3,4-dihydroxybenzoic acid, the 4-hydroxycinnamic acid are separated from the skin of Arachis hypogaea by a process comprising the steps of: extracting from the skin of Arachis hypogaea by methanol; distributing the extract by ethylacetate and a phosphoric acid buffer solution to obtain ethylacetate-soluble acidic fractions; separating the fractions by a silicagel absorption column chromatography, a sephadex LH-20 column chromatography, an octadecylsilane(ODS) column chromatography, and a HPLC.
Abstract translation: 用途：提供一种天然防腐剂，其使用含有天然抗微生物和抗氧化活性物质的花生（Arachis hypogaea）的皮肤，如3-甲氧基-4-羟基苯甲酸，3-甲氧基-4-羟基肉桂酸，4-羟基苯甲酸，3,4-二羟基苯甲酸 酸，4-羟基肉桂酸等。组成：3-甲氧基-4-羟基苯甲酸，3-甲氧基-4-羟基肉桂酸，4-羟基苯甲酸，3,4-二羟基苯甲酸，4-羟基肉桂酸 通过包括以下步骤的方法从花生中分离出酸：用甲醇从花生中提取花生; 通过乙酸乙酯和磷酸缓冲溶液分配提取物以获得乙酸乙酯可溶的酸性部分; 通过硅胶吸收柱色谱法，葡聚糖凝胶LH-20柱色谱法，十八烷基硅烷（ODS）柱色谱法和HPLC分离馏分。
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Patent Agency Ranking
公开(公告)号：KR200298047Y1
公开(公告)日：2002-12-12
申请号：KR2020020026020
申请日：2002-08-30
Applicant: 박근형
Inventor： 박근형
IPC: F16L55/115
Abstract: 본 고안은 주철관 또는 기타 금속재질의 관을 제조, 적재, 운반시 관의 내부로 이물질(흙먼지, 수분등)이 침투되는 것을 차단할 수 있도록 관의 개방구에 씌워서 사용할 수 있도록 한 것으로서, 간단한 구조를 가지고 제조비용이 저렴한 장점을 갖는 조인트관 이물 침투방지용 덮개에 관한 것이다.
이를 위해, 본 고안은 소정의 폭을 갖는 둘레판과, 이 둘레판의 일측 개방부를 마감하는 밀폐판을 서로 일체로 성형하여 요형 단면 구조를 이루게 하고, 상기 둘레판의 내경면에는 등간격을 이루는 다수개의 리브를 일체로 형성하며, 상기 밀폐판에는 다수개의 수분 배출용 홀을 관통 형성하여서 된 것을 특징으로 하는 조인트관 이물 침투방지용 덮개를 제공한다-
公开(公告)号：KR100361542B1
公开(公告)日：2002-11-21
申请号：KR1020000007233
申请日：2000-02-16
Applicant: 박근형
IPC: A47K7/00
Abstract: 본 발명은 차엽카테킨을 포함하는 물티슈제품 및 그의 제조 방법에 관한 것으로, 차엽을 열수로 추출하고, 추출액을 정제하여 에칠아세테이트로 분배시킨 후 에칠아세테이트 분획을 농축하여 유기 용매를 제거하고 물에 녹여 동결건조시키는 단계를 포함하는 방법에 의해 제조된 차엽카테킨은 피부 화농에 관련하는 균의 생육을 억제하는 항균 효과와 자유라디칼(free radical) 소거하는 항산화효과를 가지고 있어 차엽카테킨을 포함하는 물티슈는 피부염 및 피부노화 예방효과를 갖는 위생적인 물티슈제품을 제공한다.
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48.发明公开3-메톡시-4-하이드록시벤조산,3-메톡시-4-하이드록시시나믹산, 4-하이드록시벤조산,3,4-디하이드록시벤조산, 4-하이드록시시나믹산 등을포함한 땅콩껍질을 이용한 천연보존제 失效使用含有3-甲氧基-4-羟基苯甲酸的3-甲氧基-4-羟基苯甲酸，4-羟基苯甲酸，3,4-二羟基苯甲酸，4-羟基苯甲酸的海藻酸的皮肤的天然保鲜剂
公开(公告)号：KR1020020082091A
公开(公告)日：2002-10-30
申请号：KR1020010022481
申请日：2001-04-20
Applicant: 박근형
IPC: C07C51/42
Abstract: PURPOSE: Provided is a natural preservative using the skin of Arachis hypogaea containing natural antimicrobial and antioxidant active materials such as 3-methoxy-4-hydroxybenzoic acid, 3-methoxy-4-hydroxycinnamic acid, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 4-hydroxycinnamic acid, and etc. CONSTITUTION: The 3-methoxy-4-hydroxybenzoic acid, the 3-methoxy-4-hydroxycinnamic acid, the 4-hydroxybenzoic acid, the 3,4-dihydroxybenzoic acid, the 4-hydroxycinnamic acid are separated from the skin of Arachis hypogaea by a process comprising the steps of: extracting from the skin of Arachis hypogaea by methanol; distributing the extract by ethylacetate and a phosphoric acid buffer solution to obtain ethylacetate-soluble acidic fractions; separating the fractions by a silicagel absorption column chromatography, a sephadex LH-20 column chromatography, an octadecylsilane(ODS) column chromatography, and a HPLC.
Abstract translation: 目的：提供使用含有天然抗微生物和抗氧化活性物质如三甲氧基-4-羟基苯甲酸，3-甲氧基-4-羟基肉桂酸，4-羟基苯甲酸，3,4-二羟基苯甲酸 酸，4-羟基肉桂酸等。结构式：3-甲氧基-4-羟基苯甲酸，3-甲氧基-4-羟基肉桂酸，4-羟基苯甲酸，3,4-二羟基苯甲酸，4-羟基肉桂酸 通过包括以下步骤的方法从花生链球菌的皮肤分离酸：用甲醇从花生植物提取; 用乙酸乙酯和磷酸缓冲溶液分配提取物，得到乙酸乙酯可溶性酸性馏分; 通过硅胶吸收柱色谱法，sephadex LH-20柱色谱法，十八烷基硅烷（ODS）柱色谱法和HPLC分离级分。
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49.发明公开
公开(公告)号：KR1020020076980A
公开(公告)日：2002-10-11
申请号：KR1020010017769
申请日：2001-03-29
Applicant: 박근형
Abstract: PURPOSE: A natural antioxidant substance containing 4-hydroxy-3-methoxy benzoic acid, 4-hydroxy-3-methoxy cinnamic acid, quercetin-3-0-β-D-glucopyranoside and hovenidine B6 as a new natural antioxidant active material separated from Hovenia dulcis Thunb. is provided which is expected to be used as a material of food and medicine having an anti-aging function. CONSTITUTION: Leaves and stems of Hovenia dulcis Thunb. are extracted in water, freeze dried and then extracted in methanol, followed by solvent fractionation to produce an ethyl acetate acidic fraction and neural fraction. An active material contained in the extract is then purified with a purifying means such as silica gel column chromatography, chromatography with Sephadex LH-20, octadecylsilane column chromatography, a reversed-phase high-performance liquid chromatography or the like.
Abstract translation: 目的：一种天然的抗氧化物质，含有4-羟基-3-甲氧基苯甲酸，4-羟基-3-甲氧基肉桂酸，槲皮素-3-O-β-D-吡喃葡萄糖苷， Ho ia is 其被预期用作具有抗衰老功能的食品和药物的材料。 构成：枸杞子的叶子和茎。 在水中萃取，冷冻干燥，然后在甲醇中萃取，随后进行溶剂分馏，得到乙酸乙酯酸性馏分和神经级分。 然后用纯化方法如硅胶柱色谱，Sephadex LH-20色谱法，十八烷基硅烷柱色谱法，反相高效液相色谱法等纯化提取物中所含的活性物质。
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50.发明公开
公开(公告)号：KR1020010003889A
公开(公告)日：2001-01-15
申请号：KR1019990024378
申请日：1999-06-22
Applicant: 박근형
IPC: A01N65/08
Abstract: PURPOSE: A preparation method of gardenia fruit chrysophyll extract having inhibition activity of growth of microorganism such as Staphylococcus aureus, E. coli, and containing 3,4-dihydroxybenzoic acid is provided. CONSTITUTION: A preparation method of gardenia fruit chrysophyll extract is characterized by comprising the following steps: (i)grinding the dried gardenia fruit; (ii)washing by dipping in hexane solvent at room temperature for 24 hours so as to remove impurity and fat soluble component; (iii)after dipping residue in water, ethanol, methanol solvent, filtrating to obtain extract; (iv)swelling extract with water or ethanol or methanol solvent by using Sephadex LH-20 and eluting and fractionizing; (v)purifying and manufacturing gardenia fruit chrysophyll extract by collecting eluate fraction whose Ve/Vt(ratio of elution volume to total column volume) is 0.8-1.2; (vi)performing vacuum concentration or freeze drying.
Abstract translation: 目的：提供具有抑制金黄色葡萄球菌，大肠杆菌，含有3,4-二羟基苯甲酸等微生物生长抑制活性的栀子果汁提取物的制备方法。 构成：栀子果汁提取物的制备方法的特征在于包括以下步骤：（i）研磨干栀子果实; （ii）通过在己烷溶剂中浸渍在室温下洗涤24小时，以除去杂质和脂溶性组分; （iii）将残留物浸在水，乙醇，甲醇溶剂中，过滤得到提取物; （iv）使用Sephadex LH-20用水或乙醇或甲醇溶剂溶胀提取物，洗脱分级; （v）通过收集Ve / Vt（洗脱体积与总柱体积的比例）为0.8-1.2的洗脱液级分来纯化和制备栀子果汁黄酮提取物; （vi）进行真空浓缩或冷冻干燥。
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