公开(公告)号：AU6514700A

公开(公告)日：2001-02-19

申请号：AU6514700

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： HADD ANDY ,  JOVANOVICH STEVAN

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

公开(公告)号：DE3583050D1

公开(公告)日：1991-07-11

申请号：DE3583050

申请日：1985-02-19

Applicant: MOLECULAR DYNAMICS

Inventor： CARLSSON KJELL STURE

IPC: H01J37/22 ,  G01N21/59 ,  G02B21/00 ,  G02B21/36 ,  G02B27/40 ,  G03B15/00 ,  G03B17/48

公开(公告)号：AU4697196A

公开(公告)日：1996-07-31

申请号：AU4697196

申请日：1996-01-11

Applicant: MOLECULAR DYNAMICS INC

Inventor： NORRIS MICHAEL C

IPC: B01L3/00 ,  B01L9/00 ,  G01N21/13 ,  G01N35/02 ,  G01N21/01

公开(公告)号：AU3963595A

公开(公告)日：1996-06-26

申请号：AU3963595

申请日：1995-10-20

Applicant: MOLECULAR DYNAMICS

Inventor： KAIN ROBERT C

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  H01J3/14

Abstract: A fluorescence imaging system that includes an objective that is both achromatic and has an external entrance pupil. The objective also serves as a condenser for the system which substantially reduces the system's cost and footprint. With the objective positioned above a sample so that they are in close proximity to one another, a laser directs a collimated beam of light to a scan device located at the objective entrance pupil. The scan device reflects, refracts, or diffracts the light through the lens to illuminate a spot on the sample's surface. The scan device illuminates a line or an area on the sample surface by varying the angle of laser light, in one or two dimensions, into the objective. The sample emits fluorescent light in response to the illumination. The fluorescence light is collected by the objective and passes through the system along the path of the illumination light. A wavelength-discriminating dichroic filter is placed along the optical axis between the laser and the objective to direct the fluorescent light onto a photo-detector to produce a signal representing the sample surface emitting the fluorescent light. A display device is provided that displays the digitized data in a raster format.

公开(公告)号：AU3958995A

公开(公告)日：1996-05-06

申请号：AU3958995

申请日：1995-10-18

Applicant: MOLECULAR DYNAMICS

Inventor： CARLSON JEFFREY D ,  FERNANDES JORGE M

IPC: G06F9/44 ,  G06F15/00

Abstract: A method and apparatus for causing a computer to drive one or more external devices is provided. A computer executes instructions which cause the computer to display an experiment design region and a set of icons. A user copies the icons into the experiment design region to specify the various phases of an experiment. By setting controls in windows associated with the icons, a user specifies how the general stages of an experiment will be performed. Specifically, a user operates controls associated with the icons in the experiment design region to specify which devices will be used in the experiment, how data generated during the experiment will be stored, how different sets of data are related, calculations to be performed on the data, and the format of reports generated based on the data gathered during the experiment. The user specifies the operations to be performed by constructing an icon sequence in a scheduler window. The icon sequence may include device icons which represent devices, operation icons which represent the operations to be performed, and sequence control icons which affect the sequencing of certain designated operations. The computer then issues control signals to the devices specified in the icon sequence to cause the devices to perform the operations specified in the icon sequence. The operations are performed in the order specified by the icon sequence.

公开(公告)号：DE69530072T2

公开(公告)日：2004-03-04

申请号：DE69530072

申请日：1995-10-20

Applicant: MOLECULAR DYNAMICS SUNNYVALE

Inventor： KAIN C

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  H01J3/14

Abstract: A fluorescence imaging system that includes an objective that is both achromatic and has an external entrance pupil. The objective also serves as a condenser for the system which substantially reduces the system's cost and footprint. With the objective positioned above a sample so that they are in close proximity to one another, a laser directs a collimated beam of light to a scan device located at the objective entrance pupil. The scan device reflects, refracts, or diffracts the light through the lens to illuminate a spot on the sample's surface. The scan device illuminates a line or an area on the sample surface by varying the angle of laser light, in one or two dimensions, into the objective. The sample emits fluorescent light in response to the illumination. The fluorescence light is collected by the objective and passes through the system along the path of the illumination light. A wavelength-discriminating dichroic filter is placed along the optical axis between the laser and the objective to direct the fluorescent light onto a photo-detector to produce a signal representing the sample surface emitting the fluorescent light. A display device is provided that displays the digitized data in a raster format.

公开(公告)号：GB0200348D0

公开(公告)日：2002-02-20

申请号：GB0200348

申请日：2000-07-11

Applicant: MOLECULAR DYNAMICS INC

IPC: B01J19/00 ,  C12M1/00 ,  C12Q1/68 ,  C40B60/14 ,  G01N27/447

公开(公告)号：AU3088201A

公开(公告)日：2001-08-14

申请号：AU3088201

申请日：2001-01-30

Applicant: MOLECULAR DYNAMICS INC

Inventor： PENN SHARRON G ,  HANZEL DAVID K ,  CHEN WENSHENG ,  RANK DAVID R

IPC: A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/68 ,  G16B45/00

Abstract: Abstract Methods and apparatus for predicting, confirming and displaying functional regions from genomic sequence data are presented. The methods and apparatus are particularly useful for predicting coding regions within genomic sequence data, confirming the expression thereof experimentally, and relating and displaying the expression data in meaningful relationship to the genomic sequence. The methods and apparatus of the present invention thus present powerful tools for novel gene discovery.

公开(公告)号：AU3088101A

公开(公告)日：2001-08-14

申请号：AU3088101

申请日：2001-01-30

Applicant: MOLECULAR DYNAMICS INC

Inventor： PENN SHARRON G ,  HANZEL DAVID K ,  CHEN WENSHENG ,  RANK DAVID R

IPC: A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/68 ,  G16B45/00

Abstract: Abstract Methods and apparatus for predicting, confirming and displaying functional regions from genomic sequence data are presented. The methods and apparatus are particularly useful for predicting coding regions within genomic sequence data, confirming the expression thereof experimentally, and relating and displaying the expression data in meaningful relationship to the genomic sequence. The methods and apparatus of the present invention thus present powerful tools for novel gene discovery.

公开(公告)号：CA2379969A1

公开(公告)日：2001-02-08

申请号：CA2379969

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： ROACH DAVID J ,  HADD ANDREW G ,  JOVANOVICH STEVAN B ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation o r temperature cycling. The reaction containers may be filled by capillary acti on and dispensed by air displacement, centrifugal force or other means. Reactio n mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reactio n container and a second mixture component pumped of drawn into the container to form a final reaction mixture.