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公开(公告)号：KR1020100049338A
公开(公告)日：2010-05-12
申请号：KR1020080108454
申请日：2008-11-03
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A novel plant constitutive promoter is provided to introduce expression of herbicide resistant gene and reporter gene and to develop a transgenic plant. CONSTITUTION: A plant constitutive promoter contains partial or whole base of sequence number 1. The constitutive promoter is derived from Arabidopsis thaliana. The plant constitutive promoter UK2 is produced by amplifying nucleotides of sequence numbers 6 and 7 through PCR. A recombinant expression vector pBGWFS7-PAtUK2(deposit number: KACC 95085P) contains the plant constitutive promoter UK2. A method for constitutively expressing a target protein in a plant body comprises: a step of preparing the plant expression vector with the constitutive promoter UK2; a step of transforming plant carrier with the plant expression vector; and a step of introducing transgenic plant carrier into a plant body.
Abstract translation: 目的：提供一种新型植物组成型启动子，以引入除草剂抗性基因和报告基因的表达，并开发转基因植物。 构成：植物组成型启动子含有序列号1的部分或全部碱基。组成型启动子来源于拟南芥。 通过PCR扩增序列号6和7的核苷酸来产生植物组成型启动子UK2。 重组表达载体pBGWFS7-PAtUK2（保藏号：KACC 95085P）含有植物组成型启动子UK2。 在植物体中组成型表达靶蛋白的方法包括：用组成型启动子UK2制备植物表达载体的步骤; 用植物表达载体转化植物载体的步骤; 以及将转基因植物载体引入植物体内的步骤。
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公开(公告)号：KR1020100049337A
公开(公告)日：2010-05-12
申请号：KR1020080108453
申请日：2008-11-03
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A novel plant constitutive promoter is provided to express a foreign gene regardless of expression place and to induce constitutive expression of herbicide resistant gene and reporter gene. CONSTITUTION: A plant constitutive promoter UK1 contains a partial or whole base of sequence of sequence number 1. The constitutive promoter is derived from Arabidopsis thaliana. The constitutive promoter UK1 is obtained through PCR of oligonucleotide of sequence numbers 6 and 7. A recombinant expression vector contains the constitutive promoter. The recombinant expression vector is pBGWFS7-PAtUK1(deposit number: KACC 95084P). A transgenic plant is transformed the recombinant expression vector containing the constitutive promoter.
Abstract translation: 目的：提供一种新颖的植物组成型启动子，以表达外源基因，不论其表达情况如何，并诱导除草剂抗性基因和报道基因的组成型表达。 构成：植物组成型启动子UK1含有序列1序列的部分或全部碱基。组成型启动子来源于拟南芥。 通过PCR序列号6和7的寡核苷酸获得组成型启动子UK1。重组表达载体含有组成型启动子。 重组表达载体为pBGWFS7-PAtUK1（保藏号：KACC 95084P）。 转基因植物转化含有组成型启动子的重组表达载体。
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公开(公告)号：KR1020090122822A
公开(公告)日：2009-12-01
申请号：KR1020080048817
申请日：2008-05-26
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A root-specific expression promoter is provided to produce a useful protein and a functional material of regulating metabolism. CONSTITUTION: A root-specific promoter(PAtF10Ext) comprises a gene sequence of the sequence number 1 (SEQ ID NO:1). A root-specific promoter(PAtF10Ext) a promoter of gene with partial homology with extension-like protein having ample proline isolated from Arabidopsis thaliana. A primer for amplifying the root-specific promoter comprises an oligonucleotide of the sequence number 2 and oligonucleotide of the sequence number 3. A plant which is transformed by the promoter includes Arabidopsis thaliana and similar dicotyledoneae.
Abstract translation: 目的：提供根特异性表达启动子以产生有用的蛋白质和调节代谢的功能材料。 构成：根特异性启动子（PAtF10Ext）包含序列号1（SEQ ID NO：1）的基因序列。 根特异性启动子（PAtF10Ext）与具有从拟南芥分离的大量脯氨酸的延伸样蛋白具有部分同源性的基因启动子。 用于扩增根特异性启动子的引物包含序列号2的寡核苷酸和序列号3的寡核苷酸。由启动子转化的植物包括拟南芥和类似的双子叶植物。
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公开(公告)号：KR100803390B1
公开(公告)日：2008-02-14
申请号：KR1020060086012
申请日：2006-09-07
Applicant: 대한민국(농촌진흥청장)
Abstract: A root specific promoter is provided to be able to be effectively used for mass-producing useful proteins in a transgenic storage root tissue and controlling the metabolism of a storage root tissue and the production of a functional material using a transformant by root-specifically inducing a target protein gene such as genetically modified proteins or proteins introduced by a foreign useful gene. A nucleoside phosphatase promoter includes a sequence of SEQ ID : NO. 1 and root-specifically expresses. A primer for amplifying the nucleoside phosphatase promoter consists of sequences of SEQ ID : NOs. 6 and 7. A foreign protein expression vector is characterized in that the nucleoside phosphatase promoter is introduced and further comprises a bar of SEQ ID : NO. 16 or an Egfp:gus of SEQ ID : NO. 17. A transgenic plant is characterized in that the foreign protein expression vector is transformed.
Abstract translation: 提供根特异性启动子以能够有效地用于在转基因储存根组织中批量生产有用的蛋白质，并且通过根特异性诱导转化子控制存储根组织的代谢和产生功能材料 目标蛋白质基因如由外源有用基因引入的遗传修饰蛋白或蛋白质。 核苷磷酸酶启动子包括SEQ ID NO： 1和根特异性表达。 用于扩增核苷磷酸酶启动子的引物由SEQ ID NO：的序列组成。 外来蛋白质表达载体的特征在于引入核苷磷酸酶启动子，并进一步包含SEQ ID NO： 16或Egfp：gus的SEQ ID NO： 转基因植物的特征在于转化外源蛋白质表达载体。
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45.发明公开녹차 어린잎 유래 델타-6 불포화효소 유전자, 이 유전자를포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체및 이 형질전환체의 제조방법 有权绿茶嫩叶衍生的Δ-6去饱和酶基因，含有该基因的表达载体，用该表达载体转化的转化体以及该转化体的制备方法
公开(公告)号：KR1020060079397A
公开(公告)日：2006-07-06
申请号：KR1020040117577
申请日：2004-12-31
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 녹차 어린잎 유래 델타-6 불포화효소 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것으로서, 더욱 상세하게는, 서열번호 1로 기재되는 염기서열로 이루어지는, 녹차 어린잎 유래 델타-6 불포화효소를 코딩하는 유전자, 이 유전자를 포함하는 발현벡터 pYES2/CT-D6DES, 이 발현벡터로 형질전환된 효모(
Saccharomyces cerevisiae ) 형질전환체(수탁번호: KACC 93021) 및 이 형질전환체의 제조방법에 관한 것이다.
델타-6 불포화효소, pYES2/CT, Saccharomyces cerevisiaeAbstract translation: 本发明是绿茶叶得到的小增量-6不饱和基因，涉及一种表达载体，制造方法的转化，并用含有该基因的表达载体转化材料转化的转化体，更具体地，SEQ ID NO： 由以第所示的碱基序列的1，转化的酵母到编码酶的来自茶的嫩叶增量-6基因不饱和的，表达包含基因载体pYES2 / CT-D6DES，表达载体（
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Patent Agency Ranking
公开(公告)号：KR1020040084189A
公开(公告)日：2004-10-06
申请号：KR1020030019072
申请日：2003-03-27
Applicant: 대한민국(농촌진흥청장)
IPC: A01H5/00
CPC classification number: C12N15/8274 , A01H4/005 , A01H4/008 , C12N15/8205 , C12N15/8209 , C12Q1/686 , C12Q1/6895
Abstract: PURPOSE: A method for producing a herbicide-resistant chili pepper plant is provided, thereby increasing the production of a chili pepper plant and breeding a novel chili pepper plant within a short time using the herbicide-resistant chili pepper plant as a selection marker. CONSTITUTION: The method for producing a herbicide-resistant chili pepper plant comprises the steps of: (A) constructing an expression vector for plants containing Basta resistant bar gene, pCK-Bar; (B) transforming Agrobacterium tumefaciens with the expression vector to obtain transformed Agrobacterium tumefaciens; (C) co-culturing the transformed Agrobacterium tumefaciens with a chili pepper plant; (D) selecting transformed shoots from the cotyledon of the co-cultured chili pepper plant and elongating the transformed shoots; and (E) developing roots from the elongated transformed shoots.
Abstract translation: 目的：提供一种生产除草剂辣椒植物的方法，从而增加辣椒植物的产量，并在短时间内使用除草剂辣椒植物作为选择标记种植新颖的辣椒植物。 构成：生产除草剂辣椒植物的方法包括以下步骤：（A）构建含有Basta抗性bar基因pCK-Bar的植物的表达载体; （B）用表达载体转化根癌土壤杆菌以获得转化的根癌土壤杆菌; （C）用辣椒植物共培养转化的根癌土壤杆菌; （D）从共培养的辣椒植物的子叶选择转化的芽，并延长转化的芽; 和（E）从细长的转化芽发育根。
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公开(公告)号：KR101820069B1
公开(公告)日：2018-02-28
申请号：KR1020160116260
申请日：2016-09-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/825 , A01H5/10
Abstract: 본발명은식물의플라보노이드및 루테인함량을증진시키는벼() 유래의유전자(서열번호 1),유전자(서열번호 2) 및벼 최적화 FMDV 유래 2A 서열 (st2A)를포함한폴리뉴클레오티드(서열번호 3) 를포함하는이의용도에관한것으로, 더욱상세하게는벼 유래의유전자(서열번호 1) 및유전자(서열번호 2) 및벼 최적화 FMDV 유래 2A 서열 (st2A)를포함한폴리뉴클레오티드(서열번호 3)를포함하는재조합벡터를식물세포에형질전환시켜유전자(서열번호 1) 및유전자(서열번호 2)를과발현하는단계를포함하는식물의플라보노이드및 루테인함량이증진된형질전환식물체의제조방법, 상기방법에의해제조된형질전환식물체및 이의종자, 및벼 유래의유전자(서열번호 1),유전자(서열번호 2) 및벼 최적화 FMDV 유래 2A 서열 (st2A)를포함한폴리뉴클레오티드(서열번호 3)를포함하는재조합벡터를함유하는식물의플라보노이드생합성을위하여식물체의대사과정의조절의목적으로사용될수 있을뿐만아니라, 이로인하여식물유래의서로다른두가지대사물질인플라보노이드및 루테인함량증진목적으로사용될수 있을뿐 아니라식물의플라보노이드및 루테인함량증진용조성물에관한것이다.
Abstract translation: 基因（SEQ ID NO：2）包含原点2A序列mitbyeo优化FMDV核苷酸（ST2A）：用于增强从（1 SEQ ID NO）衍生的植物（）基因的类黄酮和叶黄素含量本发明水稻植物（SEQ ID NO：3）的实例包括 涉及的是，更具体地，含有大米衍生的基因的重组（SEQ ID NO：1）和基因（SEQ ID NO：2）mitbyeo优化FMDV衍生含2A序列（ST2A）多核苷酸（SEQ ID NO：3） 通过在植物细胞中（SEQ ID NO：1）转化基因的载体和基因（SEQ ID NO：2）的植物的类黄酮包括以下步骤的过表达和促进产生植物的转化方法中，通过上述方法制造的叶黄素含量 转基因植物及其种子，从mitbyeo的基因（SEQ ID NO：1）衍生的基因（SEQ ID NO：2）从含有（ST2A）的多核苷酸衍生出mitbyeo优化FMDV 2A序列（SEQ ID NO：3）的重组载体，其包含一个 它不仅被用于植物代谢过程对植物的类黄酮的生物合成，这导致，因为它可以用作类黄酮和叶黄素含量的增强而衍生自植物如在植物中黄酮类和叶黄素在一起的两个不同的代谢物的调节的目的 还有一个改进内容的作品。
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公开(公告)号：KR101696678B1
公开(公告)日：2017-01-17
申请号：KR1020140134087
申请日：2014-10-06
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은안토시아닌색소합성에관여하는유전자를센스및 안티센스방향으로포함하는식물형질전환용벡터에관한것이다. 본발명은또한상기벡터로형질전환된플라보노이드생산이증가된형질전환식물체및 식물종자에관한것이다. 본발명은또한 1) 서열번호 1, 서열번호 2 또는서열번호 3의염기서열을갖는유전자를센스및 안티센스방향으로삽입한형질전환용발현벡터를제조하는단계; 2) 상기제조된발현벡터를이용하여식물형질전환용아그로박테리움을제조하는단계; 및 3) 상기형질전환용아그로박테리움을이용하여벼를형질전환하는단계;를포함하는것을특징으로하는플라보노이드생산이증가된형질전환벼의제조방법에관한것이다.
Abstract translation: 本发明涉及用于转化植物的载体，包括在有义和反义方向上呈现并参与花青素颜料合成的基因。 此外，本发明涉及具有增加的类黄酮生产力并由所述载体转化的转基因植物及其种子。 本发明还涉及一种具有增加的类黄酮生产力的转基因水稻的制备方法，其包括以下步骤：（1）产生用于转化的表达载体，其中插入序列号为1,2或3的基因插入 并且在某种意义上和反义方向呈现; （2）通过使用产生的表达载体产生用于转化植物的农杆菌; 和（3）使用用于转化的农杆菌转化稻。
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公开(公告)号：KR101459584B1
公开(公告)日：2014-11-12
申请号：KR1020130044797
申请日：2013-04-23
Applicant: 대한민국(농촌진흥청장)
IPC: A01H5/00 , C12N15/29 , C07K14/415 , C12N15/82
Abstract: 본 발명은 진홍무로부터 분리한
신규한 RsMYB1 및 이를 형질전환한 식물체의 안토시아닌 색소 축적량 증가 효과에 관한 것으로, 안토시아닌 생합성에 관련된 MYB 타입 전사인자인 신규한 RsMYB1 유전자로 형질전환된 형질전환 식물체에서 안토시아닌 색소의 축적이 증가하였으며, 안토시아닌 생합성 관련 유전자들의 발현이 증가하였으므로 이를 안토시아닌 색소의 생합성 및 생산에 이용할 수 있다.-
公开(公告)号：KR101120603B1
公开(公告)日：2012-06-27
申请号：KR1020090031765
申请日：2009-04-13
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A beta-carotene hydroxylase 1 gene promoter and expression vector having the same are provided to induce expression of target protein through whole plant body. CONSTITUTION: An AtBch1 promoter for inducing constitutive expression comprises a nucleotide sequence of sequence number 1. A recombinant vector for inducing constitutive expression contains the promoter. The vector is pBGWFS7-PAtBch1 vector(KACC 95096P). A transgenic plant is prepared using transformed cells and the vector.
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