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    벼 줄무늬잎마름병에 대한 저항성을 증진시키는 유전자 및 이의 용도
    41.
    发明授权
    벼 줄무늬잎마름병에 대한 저항성을 증진시키는 유전자 및 이의 용도 有权
    增加对水稻条纹病抗性的基因及其用途

    公开(公告)号：KR101120458B1

    公开(公告)日：2012-06-27

    申请号：KR1020090099069

    申请日：2009-10-19

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 황덕주 ,  장지영 ,  노태환 ,  박상렬 ,  김민갑 ,  배신철

    IPC: C12N15/29 ,  C12N15/82 ,  A01H1/00

    CPC classification number: C07K14/415 ,  C12N15/8261 ,  C12N15/8279 ,  Y02A40/146

    Abstract: 본 발명은
    OsLRP
    유전자가 도입되어 벼 줄무늬잎마름병에 대한 저항성이 증진된 형질전환 식물체에 관한 것으로, 더욱 상세하게는 벼 유래의
    OsLRP (
    Oryza
    sativa leucine rich repeat protein)
    유전자를 포함하는 재조합 벡터로 형질전환된 벼 줄무늬잎마름병에 대한 저항성이 증진된 식물체 및 이의 종자, 상기
    OsLRP
    유전자를 이용하여 벼 줄무늬잎마름병에 대한 저항성이 증진된 벼 식물체의 제조 방법, 상기
    OsLRP
    유전자를 이용하여 벼 줄무늬잎마름병에 대한 저항성을 증진시키는 방법, 상기
    OsLRP
    유전자를 포함하는 식물체의 벼 줄무늬잎마름병에 대한 저항성 증진용 조성물 및 상기
    OsLRP
    유전자를 이용하여 식물체의 생산성을 향상시키는 방법에 관한 것이다.
    벼 줄무늬잎마름병, 병 저항성, 벼, 식물체, OsLRP, 형질전환체

    ＯｓＷＲＫＹ６유전자를 과량 발현시켜 식물 병 저항성을증진시키는 방법, 이러한 활성을 가진 ＯｓＷＲＫＹ６유전자, 이 유전자를 포함하는 발현벡터 및 형질전환체
    42.
    发明授权
    ＯｓＷＲＫＹ６유전자를 과량 발현시켜 식물 병 저항성을증진시키는 방법, 이러한 활성을 가진 ＯｓＷＲＫＹ６유전자, 이 유전자를 포함하는 발현벡터 및 형질전환체 有权
    通过OSWRKY6基因的超表达，具有该活性的OSWRKY6基因，含有相同基因的表达载体和转基因植物细胞提高对植物病害的抗性的方法

    公开(公告)号：KR100809671B1

    公开(公告)日：2008-03-05

    申请号：KR1020060091525

    申请日：2006-09-21

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 황덕주 ,  이데레사 ,  서석철

    IPC: C12N15/29 ,  C12N15/09 ,  C12N15/10

    Abstract: A method for enhancing the resistance against plant diseases and a transformant over-expressing an OsWRKY6 gene are provided to be utilized as a molecular breeding material for developing a disease-resistant crop by having excellent bacterial blight resistance in rice as well as resistance against wide range of pathogenic bacteria range resistance, be used for various crops because other crops preserve well disease defense mechanism relating to the OsWRKY6 gene and decrease the use of pesticide due to the disease resistant species breeding, thereby improving the productivity and supplying safe agricultural products. A method for enhancing the resistance against plant diseases comprises the steps of: (a) preparing an expression vector pB2GW7-OsWRKY6 having a cleavage map depicted in Fig. 4b including an OsWRKY6 gene consisting of SEQ ID : NO. 1; and (b) introducing the expression vector into a plant cell to prepare a transformant over-expressing the OsWRKY6 gene. A transformant is transformed by the expression vector pB2GW7-OsWRKY6, wherein the transformant is selected from the group consisting of a rice cell, a cabbage cell, a hot pepper cell, a potato cell and a rape cell. Further, the plant diseases are selected from a group consisting of Bacterial blight, rice blast disease, Rice stripe tenuivirus, Rhizoctonia solani, Rice Black-Streaked Dwarf Virus and Mycosphaerella leaf spot.

    Abstract translation: 提供了增强抗病性的方法和过表达OsWRKY6基因的转化体，用作通过在水稻中具有优异的抗病性以及耐宽泛的种类来开发抗病作物的分子育种材料 的病原菌种类抗性，用于各种作物，因为其他作物保存了与OsWRKY6基因相关的良好的疾病防治机制，并且由于抗病种的繁殖而减少了农药的使用，从而提高了生产力并提供了安全的农产品。 一种提高对植物病害抗性的方法包括以下步骤：（a）制备具有图1所示切割图的表达载体pB2GW7-OsWRKY6。 4b包括由SEQ ID NO：1组成的OsWRKY6基因。 1; 和（b）将表达载体导入植物细胞以制备过表达OsWRKY6基因的转化体。 通过表达载体pB2GW7-OsWRKY6转化转化体，其中转化体选自水稻细胞，卷心菜细胞，辣椒细胞，马铃薯细胞和强奸细胞。 此外，植物疾病选自细菌性枯萎病，稻瘟病，水稻条纹病毒，立枯丝核菌，水稻黑条纹矮病毒和丝状霉病叶斑病。

    벼에서 병 방어유전자군의 발현을 조절하는 전사인자유전자 및 이를 이용하여 벼의 획득저항성을 강화시키는방법
    45.
    发明授权
    벼에서 병 방어유전자군의 발현을 조절하는 전사인자유전자 및 이를 이용하여 벼의 획득저항성을 강화시키는방법 有权
    벼에서병방어유전자군의발현을조절하는전사인자유전자및이를이용하여벼의획득저항성을강화시키는방벼

    公开(公告)号：KR100382167B1

    公开(公告)日：2003-04-26

    申请号：KR1020000048358

    申请日：2000-08-21

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 황덕주 ,  허성기 ,  배신철 ,  변명옥 ,  고승주 ,  류진창

    IPC: C12N15/11

    Abstract: PURPOSE: Provided are gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene. In particular, provided is Oryza sativa ethylene response element binding protein(OsEREBP), a transcription factor which regulates the expression of defense related genes in rice. CONSTITUTION: The method of selecting OsEREBP comprises: treating the known systemic acquired resistance inducer, benzo-1,2,3-thiadiazole-7-carbothioic acid-S-methyl ether (BTH) to produce mRNA which is related to induction of systemic acquired resistance; transcribing the mRNA to obtain cDNA clone by using a transcriptase; selecting cDNA clone capable of being amplified by BTH treatment using the cDNA as a probe; and confirming a OsEREBP by analyzing nucleotide sequence and homology of cDNA clone. The nucleotide sequence of OsEREBP is represented by SEQ ID NO:1.

    Abstract translation: 目的：提供了调节水稻中防御相关基因表达的转录因子的基因和通过使用该基因诱导系统获得性抗性的方法。 具体而言，提供了水稻（Oryza sativa）乙烯应答元件结合蛋白（OsEREBP），其是调节水稻中防御相关基因表达的转录因子。 构成：选择OsEREBP的方法包括：处理已知的系统获得性抗性诱导剂苯并-1,2,3-噻二唑-7-硫代羧酸-S-甲基醚（BTH）以产生与诱导系统获得性有关的mRNA 抵抗性; 通过使用转录酶转录mRNA以获得cDNA克隆; 使用该cDNA作为探针选择能够通过BTH处理扩增的cDNA克隆; 并通过分析cDNA克隆的核苷酸序列和同源性来确认OsEREBP。 OsEREBP的核苷酸序列由SEQ ID NO：1表示。

    벼에서 병 방어유전자군의 발현을 조절하는 전사인자유전자 및 이를 이용하여 벼의 획득저항성을 강화시키는방법
    46.
    发明公开
    벼에서 병 방어유전자군의 발현을 조절하는 전사인자유전자 및 이를 이용하여 벼의 획득저항성을 강화시키는방법 有权
    用于调节表达防御相关基因的转录因子的基因编码及其使用方法诱发系统获得的抗性

    公开(公告)号：KR1020020015179A

    公开(公告)日：2002-02-27

    申请号：KR1020000048358

    申请日：2000-08-21

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 황덕주 ,  허성기 ,  배신철 ,  변명옥 ,  고승주 ,  류진창

    IPC: C12N15/11

    CPC classification number: C12N15/113 ,  C12N15/8216

    Abstract: PURPOSE: Provided are gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene. In particular, provided is Oryza sativa ethylene response element binding protein(OsEREBP), a transcription factor which regulates the expression of defense related genes in rice. CONSTITUTION: The method of selecting OsEREBP comprises: treating the known systemic acquired resistance inducer, benzo-1,2,3-thiadiazole-7-carbothioic acid-S-methyl ether (BTH) to produce mRNA which is related to induction of systemic acquired resistance; transcribing the mRNA to obtain cDNA clone by using a transcriptase; selecting cDNA clone capable of being amplified by BTH treatment using the cDNA as a probe; and confirming a OsEREBP by analyzing nucleotide sequence and homology of cDNA clone. The nucleotide sequence of OsEREBP is represented by SEQ ID NO:1.

    Abstract translation: 目的：提供调节水稻防御相关基因表达的转录因子的基因编码和使用该基因诱导系统性获得性抗性的方法。 特别地，提供了水稻乙烯应答元件结合蛋白（OsEREBP），一种调节水稻防御相关基因表达的转录因子。 构成：选择OsEREBP的方法包括：治疗已知的系统获得性阻力诱导剂，苯并1,2,3-噻二唑-7-硫代硫酸S-甲基醚（BTH），产生与诱导全身获得的mRNA相关的mRNA 抵抗性; 通过使用转录酶转录mRNA以获得cDNA克隆; 选择能够通过使用cDNA作为探针的BTH处理扩增的cDNA克隆; 并通过分析cDNA克隆的核苷酸序列和同源性来确认OsEREBP。 OsEREBP的核苷酸序列由SEQ ID NO：1表示。

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