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1.发明授权벼에서 병 방어유전자군의 발현을 조절하는 전사인자유전자 및 이를 이용하여 벼의 획득저항성을 강화시키는방법 有权벼에서병방어유전자군의발현을조절하는전사인자유전자및이를이용하여벼의획득저항성을강화시키는방벼
公开(公告)号：KR100382167B1
公开(公告)日：2003-04-26
申请号：KR1020000048358
申请日：2000-08-21
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/11
Abstract: PURPOSE: Provided are gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene. In particular, provided is Oryza sativa ethylene response element binding protein(OsEREBP), a transcription factor which regulates the expression of defense related genes in rice. CONSTITUTION: The method of selecting OsEREBP comprises: treating the known systemic acquired resistance inducer, benzo-1,2,3-thiadiazole-7-carbothioic acid-S-methyl ether (BTH) to produce mRNA which is related to induction of systemic acquired resistance; transcribing the mRNA to obtain cDNA clone by using a transcriptase; selecting cDNA clone capable of being amplified by BTH treatment using the cDNA as a probe; and confirming a OsEREBP by analyzing nucleotide sequence and homology of cDNA clone. The nucleotide sequence of OsEREBP is represented by SEQ ID NO:1.
Abstract translation: 目的：提供了调节水稻中防御相关基因表达的转录因子的基因和通过使用该基因诱导系统获得性抗性的方法。 具体而言，提供了水稻（Oryza sativa）乙烯应答元件结合蛋白（OsEREBP），其是调节水稻中防御相关基因表达的转录因子。 构成：选择OsEREBP的方法包括：处理已知的系统获得性抗性诱导剂苯并-1,2,3-噻二唑-7-硫代羧酸-S-甲基醚（BTH）以产生与诱导系统获得性有关的mRNA 抵抗性; 通过使用转录酶转录mRNA以获得cDNA克隆; 使用该cDNA作为探针选择能够通过BTH处理扩增的cDNA克隆; 并通过分析cDNA克隆的核苷酸序列和同源性来确认OsEREBP。 OsEREBP的核苷酸序列由SEQ ID NO：1表示。
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2.发明公开
公开(公告)号：KR1020020015179A
公开(公告)日：2002-02-27
申请号：KR1020000048358
申请日：2000-08-21
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/11
CPC classification number: C12N15/113 , C12N15/8216
Abstract: PURPOSE: Provided are gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene. In particular, provided is Oryza sativa ethylene response element binding protein(OsEREBP), a transcription factor which regulates the expression of defense related genes in rice. CONSTITUTION: The method of selecting OsEREBP comprises: treating the known systemic acquired resistance inducer, benzo-1,2,3-thiadiazole-7-carbothioic acid-S-methyl ether (BTH) to produce mRNA which is related to induction of systemic acquired resistance; transcribing the mRNA to obtain cDNA clone by using a transcriptase; selecting cDNA clone capable of being amplified by BTH treatment using the cDNA as a probe; and confirming a OsEREBP by analyzing nucleotide sequence and homology of cDNA clone. The nucleotide sequence of OsEREBP is represented by SEQ ID NO:1.
Abstract translation: 目的：提供调节水稻防御相关基因表达的转录因子的基因编码和使用该基因诱导系统性获得性抗性的方法。 特别地，提供了水稻乙烯应答元件结合蛋白（OsEREBP），一种调节水稻防御相关基因表达的转录因子。 构成：选择OsEREBP的方法包括：治疗已知的系统获得性阻力诱导剂，苯并1,2,3-噻二唑-7-硫代硫酸S-甲基醚（BTH），产生与诱导全身获得的mRNA相关的mRNA 抵抗性; 通过使用转录酶转录mRNA以获得cDNA克隆; 选择能够通过使用cDNA作为探针的BTH处理扩增的cDNA克隆; 并通过分析cDNA克隆的核苷酸序列和同源性来确认OsEREBP。 OsEREBP的核苷酸序列由SEQ ID NO：1表示。
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公开(公告)号：KR1019950018484A
公开(公告)日：1995-07-22
申请号：KR1019930030237
申请日：1993-12-28
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 한우 품종을 수입 소 또는 젖소의 품종으로부터 판별하기 위한 DNA 다형성 분석법에 사용하기 위한 DNA 단편 및 이 단편을 이용하여 한우 품종을 수입 소 또는 젖소의 품종으로부터 판별하는 방법을 제공한다.
본 발명에 의해 제공되는 프라이머는 염기 배열 AAGGTCCTAGGGTCCTGGG의 전부 혹은 일부를 포함하거나 또는 이 배열과 균등한 DNA 단편이며; 본 발명에 따른 한우 품종의 판별방법은 상기 프라이머를 피험 소의 DNA 와 접촉시키고, 상기 프라이머가 부착된 피험 소의 DNA 단편을 검출해냄을 특징으로 한다. 이 판별방법에서는 프라이머 또는 프로브 DNA 단편과 피험 소의 DNA 단편을 41℃이하의 온도에서 30초-100초간 접촉 시키며, 피험 소의 DNA 는 혈액 또는 고기 또는 기타 다른 부위로부터 채취할 수 있다. 이 판별방법에 의해서 프라이머 단편과 부착된 DNA 단편을 갖는 것으로 판명된 소는 한우 품종이 아닌 젖소 또는 수입 소 품종이다.
따라서, 본 발명의 방법에 의하면 신속하고 간단하게, 그리고 정확하게 한우 또는 한우 고기를 젖소 또는 수입 소 또는 그의 고기로부터 판별해낼 수 있다.-
4.发明授权느타리 버섯으로부터 분리된 신규한 ＧＰＤ유전자를 형질전환시킨 오스모틱 스트레스 내성 효모 有权通过过表达从蘑菇中分离的GPD基因产生渗透胁迫抗性转基因酵母Pleurotus sajor-caju
公开(公告)号：KR100325309B1
公开(公告)日：2002-02-25
申请号：KR1019990031152
申请日：1999-07-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/81
Abstract: 본발명은느타리버섯으로부터분리된 Osmotic stress 관련유전자인신규한글리세롤-3-포스페이트디하이드로지나제(이하 GPD라고약칭함)를함유한재조합벡타를이용하여형질전환시킨효모및 그형질전환방법에관한것이다. 본발명에의해얻어진형질전환효모는기존의효모에비하여염, 저온, 고온및 건조등의외부환경적인스트레스에대한저항력이탁월하게증진되어효모의효율성이높으며또한장기보존이가능할뿐만아니라상기유전자는식용버섯에서유래하였기때문에식품으로서유해성이전혀없어산업상매우유용하다. 따라서본 발명은식품제조분야등에있어서내염, 내한발성특성이요구되어지는경우에특히유용할뿐만아니라, 향후미생물을이용한발효기술에있어서작업조건에따라변화하는외부스트레스에대한능동적인대처를가능하게하는장점이있다.
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5.发明公开느타리 버섯으로부터 분리된 신규한 ＧＰＤ유전자와 상기 유전자를 형질전환시킨 오스모틱 스트레스 내성 감자 有权通过从PLEUROTUS SAJOR-CAJU分离的含有GPD基因的重组载体转化的抗氧化应激蛋白
公开(公告)号：KR1020010011674A
公开(公告)日：2001-02-15
申请号：KR1019990031153
申请日：1999-07-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/10
Abstract: PURPOSE: The osmotic stress resistant potatoes transformed by a recombinant vector containing the GPD gene isolated from Pleurotus sajor-caju is provided for improving the osmotic stress resistance of potatoes, so that the potatoes can be grown in soil slightly containing salt. CONSTITUTION: The osmotic stress resistant potatoes transformed by a recombinant vector containing glycerol-3-phosphate dehydrogenase(GPD) gene as an osmotic stress related gene is obtained by the following steps of: incubating Pleurotus sajor-caju ASI 2070 in an MCM medium containing 20g of peptone, 2g of yeast extract, 2g of K2HPO4, 1.0g of KH2PO4, 0.46g of MgSO47H2O, 0.5g of DW1L at 25deg.C for 15 days; filtering with mira cloth; separating total RNA and DNA of Pleurotus sajor-caju ASI 2070 by using a Graham method and a Sambrook method, respectively; preparing pBI vector containing the GPD gene by using restriction enzymes; infecting Agrobacterium with the pBI vector; and transforming potatoes with the pBI infected Agrobacterium.
Abstract translation: 目的：提供通过含有从侧耳属sajor-caju分离的GPD基因的重组载体转化的渗透胁迫性马铃薯，用于提高马铃薯的渗透胁迫抗性，使土豆可以在含有盐的土壤中生长。 构成：通过含有甘油-3-磷酸脱氢酶（GPD）基因的重组载体转化的渗透胁迫土豆作为渗透胁迫相关基因，通过以下步骤获得：将Pleurotus sajor-caju ASI 2070在含有20g 的蛋白胨，2g的酵母提取物，2g的K 2 HPO 4，1.0g的KH 2 PO 4，0.46g的MgSO 4·7H 2 O，0.5g的DW1L，25℃，15天; 用米拉布过滤; 分别使用Graham方法和Sambrook方法分离了侧耳菇sajor-caju ASI 2070的总RNA和DNA; 通过使用限制酶制备含有GPD基因的pBI载体; 用pBI载体感染农杆菌; 并用pBI感染的土壤杆菌转化土豆。
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Patent Agency Ranking
公开(公告)号：KR100257452B1
公开(公告)日：2000-06-01
申请号：KR1019980012807
申请日：1998-04-10
Applicant: 대한민국(농촌진흥청장)
IPC: C12N1/20
Abstract: PURPOSE: Provided is Bacillus subtilis A405 (KFCC-11024) which produces effective peptides in preventing and treating plant diseases to manufacture the peptides in a high yield. CONSTITUTION: Bacillus subtilis A405 (KFCC-11024) is obtained by the following steps of: i) separating bacteria resistant to pathogenic bacteria from soil; ii) cultivating the bacteria in an LB medium containing 1% of bactotrypton, 0.5% of bacto extracts, and 1% of NaCl at 28 deg.C for four days; iii) obtaining supernatant after centrifugation at 7,000rpm for 20 minutes; iv) adding ammonium sulphate to the supernatant to make macromolecules settle, and heating the sediments after centrifugation for 5 minutes; and v) obtaining macromolecules which are not destroyed from the supernatant and broth. The bacteria are gram-positive and have a 99% similarity to Bacillus subtilis.
Abstract translation: 目的：提供枯草芽孢杆菌A405（KFCC-11024），其产生有效的肽以预防和治疗植物疾病以高产率制造肽。 构成：通过以下步骤获得枯草芽孢杆菌A405（KFCC-11024）：i）从土壤中分离出抗病原菌的细菌; ii）在28℃下在含有1％的细菌培养基，0.5％的细菌提取物和1％的NaCl的LB培养基中培养细菌4天; iii）以7000rpm离心20分钟后得到上清液; iv）向上清液中加入硫酸铵使大分子沉降，离心5分钟后加热沉淀物; 和v）获得未从上清液和肉汤中破坏的大分子。 细菌是革兰氏阳性的，与枯草芽孢杆菌具有99％的相似性。
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公开(公告)号：KR100257451B1
公开(公告)日：2000-06-01
申请号：KR1019980011708
申请日：1998-04-02
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A freshness maintaining goods using polyglutamic acid(PGA) from Bacillus subtilis is provided, which extend the term of validity and storage of vegetables and fruits by inhibiting growth of fungus or bacteria. CONSTITUTION: A process for the preparation of freshness maintaining goods using polyglutamic acid comprises the steps of: inoculating Bacillus subtilis TB11(KFCC-11025) into the GA medium, shake-culturing at 28deg.C, 170rpm for 3days, and centrifuging the cultured solution at 10,000xg for 20minutes; adding three times of ethanol in volume to the supernatant, and keeping at 4deg.C for a night; precipitating white gelatinous material by centrifuging at 7,000xg for 15minutes, dissolving the precipitate in distilled water, and dialyzing at 4deg.C for 3days while replacing the distilled water on occasion; and freeze-drying to get polyglutamic acid powder.
Abstract translation: 目的：提供使用来自枯草芽孢杆菌的聚谷氨酸（PGA）的保鲜产品，通过抑制真菌或细菌的生长来延长蔬菜和水果的有效期和储存期。 构成：使用聚谷氨酸制备保鲜品的方法包括以下步骤：将芽孢杆菌TB11（KFCC-11025）接种到GA培养基中，以28℃，170rpm摇动培养3天，离心培养的溶液 10,000xg 20分钟; 向上清液中加入3倍体积的乙醇，并保持在4℃下一夜; 通过以7,000xg离心15分钟沉淀白色凝胶状物质，将沉淀物溶解在蒸馏水中，并在4℃下透析3天，有时更换蒸馏水; 并冷冻干燥得到聚谷氨酸粉末。
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公开(公告)号：KR100382164B1
公开(公告)日：2003-04-26
申请号：KR1020000047902
申请日：2000-08-18
Applicant: 대한민국(농촌진흥청장)
IPC: C12N1/20
Abstract: PURPOSE: A novel Bacillus sp. strain having a strong antifungal activity against a plant pathogen is provided, thereby effectively inhibiting the diseases occurring in oranges. CONSTITUTION: The Bacillus sp. YJP-1 having a strong antifungal activity against Penicillium sp. which causes a diseases in oranges is isolated from the soil, Its preparation method comprises the steps of: collecting a soil sample, spreading it on NB plate, and cultivating it at 30 deg.C for 1 to 2 days; inoculating the colonies grown in NB broth and cultivating them at 30 deg.C for 76 hours with agitation; centrifuging the culture at 12,000 g for 20 minutes to obtain the supernatant; measuring an antifungal activity of the culture by a paper disc method using Penicillium digitatum (ATCC 10030); and selecting and identifying a strain showing a strong antifungal activity.
Abstract translation: 目的：一种新型芽孢杆菌。 提供了对植物病原体具有强抗真菌活性的菌株，从而有效地抑制了在桔子中发生的疾病。 组成：芽孢杆菌属。 YJP-1对青霉菌具有很强的抗真菌活性。 从土壤中分离出导致柑橘病害的土壤，其制备方法包括以下步骤：收集土壤样品，铺在NB平板上，在30℃下培养1-2天; 接种在NB肉汤中生长的菌落并在搅拌下于30℃培养76小时; 以12,000g离心培养20分钟以获得上清液; 通过使用指状青霉（Penicillium digitatum）（ATCC 10030）的纸盘法测量培养物的抗真菌活性; 并选择和鉴定显示出强抗真菌活性的菌株。
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公开(公告)号：KR1020020014560A
公开(公告)日：2002-02-25
申请号：KR1020000047902
申请日：2000-08-18
Applicant: 대한민국(농촌진흥청장)
IPC: C12N1/20
Abstract: PURPOSE: A novel Bacillus sp. strain having a strong antifungal activity against a plant pathogen is provided, thereby effectively inhibiting the diseases occurring in oranges. CONSTITUTION: The Bacillus sp. YJP-1 having a strong antifungal activity against Penicillium sp. which causes a diseases in oranges is isolated from the soil, Its preparation method comprises the steps of: collecting a soil sample, spreading it on NB plate, and cultivating it at 30 deg.C for 1 to 2 days; inoculating the colonies grown in NB broth and cultivating them at 30 deg.C for 76 hours with agitation; centrifuging the culture at 12,000 g for 20 minutes to obtain the supernatant; measuring an antifungal activity of the culture by a paper disc method using Penicillium digitatum (ATCC 10030); and selecting and identifying a strain showing a strong antifungal activity.
Abstract translation: 目的： 提供了对植物病原体具有强抗真菌活性的菌株，从而有效地抑制了发生在橘子中的疾病。 构成：芽孢杆菌 YJP-1对青霉属具有很强的抗真菌活性。 导致橘子的疾病与土壤分离，其制备方法包括：收集土壤样品，铺在NB板上，在30℃下培养1〜2天; 接种生长在NB肉汤中的菌落，并在30℃下搅拌培养76小时; 以12,000g离心培养20分钟，得到上清液; 通过使用Penicillium digitatum（ATCC 10030）的纸盘法测量培养物的抗真菌活性; 选择和鉴定显示出强烈的抗真菌活性的菌株。
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10.发明授权
公开(公告)号：KR100267741B1
公开(公告)日：2001-03-02
申请号：KR1019980011707
申请日：1998-04-02
Applicant: 대한민국(농촌진흥청장)
IPC: C12N1/20
Abstract: PURPOSE: A bacillus subtilis TB11 strain and a method for producing polyglutamic acid(PGA) thereby are provided, therefore the produced PGA having excellent heat resistance can be effectively used in disposing of livestock wastes. CONSTITUTION: The bacillus subtilis TB11 strain(KFCC-11025) produces polyglutamic acid(PGA) having excellent heat resistance. The polyglutamic acid (PGA) is produced by fermenting Bacillus subtilis TB11(KFCC-11025) in a medium containing 2% glycerol, 2% glutamate salt, 0.15% of ammonia chloride and 1.2% of citric acid, pH 7.2. The polyglutamic acid (PGA) consists of glutamate and has the molecular weight of 10,000 kDa or more.
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