-
公开(公告)号：KR100823684B1
公开(公告)日：2008-04-21
申请号：KR1020060123353
申请日：2006-12-06
Applicant: 한국전자통신연구원
CPC classification number: C12Q1/6816 , C12Q1/6834 , C12Q1/6837 , B82Y5/00 , C12Q2563/143 , C12Q2563/185
Abstract: A method for detecting a biological target material using a barcode DNA is provided to analyze a trace amount of DNA, which is less than femto or atto mole, without an expensive amplification. A method for detecting a biological target material using a barcode DNA comprises the steps of: (a) preparing magnetic particles for isolation where a first probe having at least 30% homology to the biological target material to be detected is attached to the surface of the magnetic particles and preparing polymer particles for analysis where a second probe having at least 25% of homology to the target material and different from the first probe is attached to the surface of polymer particles and a barcode DNA, which exists in a percentage of at least three times of the second probe and has a predetermined arrangement, is attached to the surface of the polymer particles, where the barcode DNA is coupled to a marker; (b) reacting the magnetic particles for isolation, polymer particles for analysis and the target material in a hybridization reaction buffer; (c) isolating a conjugate consisting of the magnetic particles for isolation-target material-polymer particles for analysis from the reaction material using a magnetic separator; (d) applying heat to the isolated conjugate to denature the barcode DNA existing in the polymer particles for analysis and removing the magnetic particles for isolation and the polymer particles for analysis from the conjugate through centrifugation to isolate the barcode DNA; and (e) detecting a signal from the marker coupled to the isolated barcode DNA, wherein each of the target material, the first probe and the second probe is DNA, RNA or protein, respectively, and the marker is a nano-sized magnetic particle, a fluorescence analysis possible material or a material which is negatively charged on the surface thereof. Further, the magnetic particles have superparamagnitic property and are selected from a group consisting of Iron-Oxide, Iron-Cobalt, Iron-Nickel and transition element.
Abstract translation: 提供使用条形码DNA检测生物目标物质的方法，以分析微量的DNA，其小于毫微微摩尔或摩尔比，而不需要昂贵的扩增。 使用条形码DNA检测生物靶材的方法包括以下步骤：（a）制备用于分离的磁性颗粒，其中与待检测的生物目标材料具有至少30％同源性的第一探针附着到 磁性颗粒并制备用于分析的聚合物颗粒，其中具有至少25％的与靶材料同源性并且不同于第一探针的第二探针附着到聚合物颗粒的表面和条形码DNA，其以至少的百分比存在 将三次第二探针并具有预定的布置，附着到聚合物颗粒的表面，其中条形码DNA与标记偶联; （b）使用于分离的磁性颗粒，用于分析的聚合物颗粒和杂交反应缓冲液中的靶材料进行反应; （c）使用磁选机从反应材料中分离由用于分离目标材料 - 聚合物颗粒的磁性颗粒组成的共轭物用于分析; （d）向隔离的结合物施加热量以使存在于聚合物颗粒中的条形码DNA变性以分析并除去用于分离的磁性颗粒和用于通过离心从共轭物分析的聚合物颗粒以分离条形码DNA; 和（e）检测来自与分离的条形码DNA连接的标记的信号，其中目标材料，第一探针和第二探针中的每一个分别是DNA，RNA或蛋白质，并且标记是纳米尺寸的磁性颗粒 荧光分析可能的材料或在其表面上带负电的材料。 此外，磁性颗粒具有超顺反性，并且选自铁氧化物，铁 - 钴，铁 - 镍和过渡元素。
-
-
公开(公告)号：KR101904781B1
公开(公告)日：2018-10-05
申请号：KR1020160132004
申请日：2016-10-12
Applicant: 한국전자통신연구원
IPC: G01R33/12 , G01R33/00 , G01R23/167
CPC classification number: G01R33/0023 , G01R33/0029 , G01R33/12 , G01R33/32 , G01R33/34053 , G06F11/1443
Abstract: FMMD 기술의신호분석을위한신호송수신방법및 이를이용한장치가개시된다. 본발명에따른신호송신장치는마그네틱파티클측정센서에포함된여기(excitation) 코일의전압을고려하여주파수가서로상이한두 개의정현파신호들을생성하는신호생성부; 상기두 개의정현파신호들을전자적으로더하여자기장을발생시키기위한입력신호를생성하는신호가산부; 전송노이즈를최소화하기위해상기입력신호에대한디퍼런셜(differential) 신호를생성하는디퍼런셜신호생성부; 및상기여기코일에서자기장을발생시켜측정대상시료에자기장을인가하기위해상기입력신호와상기디퍼런셜신호의전압을증폭하여상기여기코일로전송하는신호전송부를포함한다.
-
公开(公告)号：KR1020150002303A
公开(公告)日：2015-01-07
申请号：KR1020130075974
申请日：2013-06-28
Applicant: 한국전자통신연구원
IPC: G01N27/72
Abstract: 본 발명에 따른 상자성 물질 분석 장치는 서로 동일한 극성이 마주보도록 배치되며, 공급된 전원에 의해 형성된 자기장을 통해 필드 프리 라인(Filed Free Line, FFL)을 생성하는 둘 이상의 전자석, 둘 이상의 전자석에 전원을 공급하며, 둘 이상의 전자석에 공급되는 전원의 출력을 조절하여 FFL의 위치를 조절하는 전원 공급부, 둘 이상의 전자석의 위치를 이동시켜 생성된 FFL의 위치를 조절하는 이송부 및 생성된 FFL 내의 상자성체로부터 방출되는 비선형 신호를 측정하는 측정부를 포함한다.
Abstract translation: 本发明的顺磁材料分析设备包括两个和更多个电磁体，其布置成使得相同的极性相互面对并通过由所供电的磁场形成的磁场产生无磁场线（FFL）; 电源单元，其通过控制提供给电磁体的功率的输出来向电磁体供电并控制FFL的位置; 传送单元，其控制通过传送电磁体的位置产生的FFL的位置; 以及测量单元，其测量由所产生的FFL内的顺磁材料产生的非线性信号。
-
公开(公告)号：KR1020140137506A
公开(公告)日：2014-12-03
申请号：KR1020130057893
申请日：2013-05-22
Applicant: 한국전자통신연구원
CPC classification number: G09G3/003 , G09G2340/0407 , H04N13/139 , H04N13/388
Abstract: ３차원 발광 장치를 이용한 데이터의 다차원 시각화 방법이 제공되며, 적어도 하나의 센서로부터 시료의 적어도 하나의 부위를 스캔한 데이터를 수집하는 단계, 3 차원 발광 장치의 해상도와 스캔한 데이터의 해상도를 비교하는 단계, 비교 결과 3 차원 발광 장치의 해상도와 스캔한 데이터의 해상도가 동일하지 않은 경우, 스캔한 데이터의 해상도를 3 차원 발광 장치의 해상도에 맞도록 조절하는 단계, 및 조절된 해상도를 가지고 시료에 대응하는 데이터를, 3 차원 발광 장치를 구동하여 디스플레이하는 단계를 포함한다.
Abstract translation: 提供了一种使用三维发光装置对数据进行多维可视化的方法，其包括以下步骤：从至少一个传感器收集至少一部分样本的扫描数据; 比较三维发光装置的分辨率和扫描数据的分辨率; 作为比较的结果，当三维发光装置的分辨率和扫描数据的分辨率不相等时，将扫描数据的分辨率调整为三维发光装置的分辨率; 并通过驱动三维发光装置显示具有调整分辨率的与样本相对应的数据。
-
Patent Agency Ranking
公开(公告)号：KR1020120020985A
公开(公告)日：2012-03-08
申请号：KR1020100084963
申请日：2010-08-31
Applicant: 한국전자통신연구원
Inventor： 홍효봉
IPC: C12Q1/68 , G01N33/553 , G01N33/545
CPC classification number: G01N33/545 , B01J19/0046 , B01J2219/005 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , G01N33/54326 , C12Q1/6834 , G01N33/553
Abstract: PURPOSE: A method for measuring bio substance using a polymer thin film and magnetic bead is provided to ensure wide analysis area and to enable array analysis. CONSTITUTION: A method for measuring a bio substance using a polymer thin film and magnetic bead comprises: a step of fixing the bio substance on the polymer thin film which is formed of nylon or nitrocellulose(S100); a step of conjugating a chemically functional group to the magnetic bead(S110); and a step of measuring the bio substance using the magnetic bead reader(S120). The bio substance is DNA or protein. The chemically functional group is cDNA.
Abstract translation: 目的：提供使用聚合物薄膜和磁珠测量生物物质的方法，以确保广泛的分析区域并实现阵列分析。 构成：使用聚合物薄膜和磁珠测量生物物质的方法包括：将生物物质固定在由尼龙或硝化纤维素形成的聚合物薄膜上的步骤（S100）; 将化学官能团与磁珠接合的步骤（S110）; 以及使用磁珠阅读器测量生物物质的步骤（S120）。 生物物质是DNA或蛋白质。 化学官能团是cDNA。
-
公开(公告)号：KR1020110091104A
公开(公告)日：2011-08-11
申请号：KR1020100010765
申请日：2010-02-05
Applicant: 한국전자통신연구원
Inventor： 홍효봉
CPC classification number: G01N33/6803 , G01N2333/11 , G01N2410/00 , G01N33/6845 , G01N33/532 , G01N33/536
Abstract: PURPOSE: A method for analyzing a protein microstructure is provided to detect novel virus of influenza A virus subtype and to sense cancer or other diseases without expensive equipment. CONSTITUTION: A method for analyzing a protein microstructure comprises: a step of fixing a capture substance on a substance regardless of microsturcture of a target substance; a step of binding the target substance and capture substance to form a first composite; a step of binding the first composite with one kind of probe molecule to form a plurality of second composites; a step of performing chromogenic reaction to the plural second composite to measure binding affinity between the first composite and probe molecule; and a step of comparing the measured binding affinity and existing binding affinity data to determine the target substance. The probe molecule is amino acid dimer. The amino acid dimer is TRP-TRP, SER-HIS, GLU-ILE, SER-PRO, ASN-GLU, GLY-TRP, MET-ALA, MEY-LYS, PHE-GLY, or ILE-LEU.
Abstract translation: 目的：提供一种分析蛋白质微结构的方法，用于检测甲型流感病毒亚型的新型病毒，并在没有昂贵设备的情况下感染癌症或其他疾病。 构成：用于分析蛋白质微结构的方法包括：将捕获物质固定在物质上的步骤，而不管目标物质的微结构如何; 结合目标物质和捕获物质以形成第一复合物的步骤; 将第一复合材料与一种探针分子结合以形成多个第二复合材料的步骤; 对多个第二复合物进行显色反应以测量第一复合材料和探针分子之间的结合亲和力的步骤; 以及比较测量的结合亲和力和现有结合亲和力数据以确定目标物质的步骤。 探针分子是氨基酸二聚体。 氨基酸二聚体是TRP-TRP，SER-HIS，GLU-ILE，SER-PRO，ASN-GLU，GLY-TRP，MET-ALA，MEY-LYS，PHE-GLY或ILE-LEU。
-
公开(公告)号：KR1020110019953A
公开(公告)日：2011-03-02
申请号：KR1020090077587
申请日：2009-08-21
Applicant: 한국전자통신연구원
CPC classification number: C12Q1/6876 , G01N33/6812
Abstract: PURPOSE: A method for analyzing a material using difference of binding affinity is provided to determine presence of a specific protein and minute changer of the specific protein. CONSTITUTION: A method for analyzing a specific protein comprises: a step of patterning a binding affinity of the specific viral protein of a substrate and plural probes containing an amino acid dimer or derivatives thereof and making database; a step of measuring binding affinity by contacting the plural probes with a sample; a step of comparing the measured binding affinity with the database; and a step of confirming the specific protein. The amino acid dimer or derivative thereof is a compound of chemical formula 1.
Abstract translation: 目的：提供使用结合亲和力差异分析材料的方法，以确定特异性蛋白质和特定蛋白质的分选器的存在。 构成：用于分析特定蛋白质的方法包括：图案化底物的特定病毒蛋白质和包含氨基酸二聚体或其衍生物的多个探针的结合亲和力并进行数据库的步骤; 通过使多个探针与样品接触来测量结合亲和力的步骤; 将测量的结合亲和力与数据库进行比较的步骤; 以及确认特定蛋白质的步骤。 氨基酸二聚体或其衍生物是化学式1的化合物。
-
公开(公告)号：KR101003534B1
公开(公告)日：2010-12-30
申请号：KR1020080114059
申请日：2008-11-17
Applicant: 한국전자통신연구원
Abstract: 본 발명은 자성나노입자와 주파수 혼합 자기 판독기(frequency mixing magnetic reader)를 이용한 생체물질의 정량적 검출방법에 관한 것이다. 본 발명의 검출방법에 의하면, 자성나노입자의 수에 대한 신호의 검출로서 간단하게 생체물질의 존재를 정량적으로 측정할 수 있고, ELISA(Enzyme-Linked ImmunoSorbent Assay) 보다 안정하면서 그와 유사한 고민감도의 신뢰성있는 데이터를 확보할 수 있다. 또한 본 발명의 검출방법에 따르면, 1회용의 스트립 유형의 기판부가 센서로 이용되므로 공정 및 비용 측면에서 매우 경제적이다.
자성나노입자, 주파수 혼합 자기 판독기, 스트립-
公开(公告)号：KR1020100073558A
公开(公告)日：2010-07-01
申请号：KR1020080132270
申请日：2008-12-23
Applicant: 한국전자통신연구원
IPC: B01L3/00
Abstract: PURPOSE: A reaction container and a micro reaction container using the same are provided to enhance reaction mount between the reaction materials. CONSTITUTION: A reaction container(101) has one or more protrusion(102) inside. The reaction container is a micro reaction container for biochemical reaction. The reaction container is a well of microplate for biochemical reaction. The protrusion is integral or separated from the reaction container. The protrusion is spherical, semi-spherical, cylindrical, conical, or trapezoidal. The micro reaction array is formed by collecting two or more reaction containers.
Abstract translation: 目的：提供反应容器和使用其的微反应容器，以增强反应材料之间的反应安装。 构成：反应容器（101）内部具有一个或多个突起（102）。 反应容器是用于生化反应的微反应容器。 反应容器是用于生化反应的微板的孔。 突起与反应容器成一体或分离。 突起是球形，半球形，圆柱形，圆锥形或梯形。 微反应阵列通过收集两个或更多个反应容器形成。
-
-
-
-
-
-
-
-
-
Search Results
53
records
(took 0.033 seconds)
