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Patent Agency Ranking42.发明公开
公开(公告)号：KR1020170140762A
公开(公告)日：2017-12-21
申请号：KR1020170025781
申请日：2017-02-27
Applicant: 한국해양과학기술원
Abstract: 본발명은남조류스피룰리나추출물을함유하는세포배양액, 이의제조방법, 및이를이용한세포의배양방법에관한것으로, 더욱상세하게는남조류스피룰리나속 시료로부터남조류스피룰리나추출물을수득한다음, 상기남조류스피룰리나추출물을여과하여제조되는남조류스피룰리나추출물을함유하는세포배양액, 이의제조방법, 및이를이용한세포의배양방법에관한것이다. 본발명에따른남조류스피룰리나추출물을함유한세포배양액(Spirulina Animal Cell Culture Solution, SACCS)은세포배양용혈청대체제로유용하게이용할수 있다.
Abstract translation: 本发明是蓝绿藻螺旋藻提取物，细胞培养物，它们的制备方法含有，并获得了蓝绿藻螺旋藻提取物涉及使用相同的，并且在样品中更特别蓝绿藻螺旋藻，然后培养细胞，过滤所述蓝绿藻螺旋藻提取物的方法 ，其生产方法，以及使用其培养细胞的方法。 含有本发明（动物细胞培养液的螺旋藻，SACCS）与蓝绿藻螺旋藻提取物的细胞培养基可以是有用地血清用于细胞培养的替代为零。
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公开(公告)号：KR101556771B1
公开(公告)日：2015-10-06
申请号：KR1020140116195
申请日：2014-09-02
Applicant: 한국해양과학기술원
Abstract: 본발명은미세조류배양용밀폐형광생물반응기에관한것이다. 본발명에따른미세조류배양용밀폐형광생물반응기는, 밀폐형구조를가지며, 그내부에미세조류를수납하여미세조류를배양하는배양수조; 배양수조에근접설치되며, 배양수조내에서배양되는미세조류에광 조건을충족시키기위해배양수조에광 에너지를제공하는조명장치; 배양수조에기계적으로연결되며, 배양수조내에서배양된미세조류의수확및 성분분석을위해미세조류를원심분리하는원심분리기; 및배양수조에기계적으로연결되며, 배양수조내에서배양된미세조류의생산량을측정하기위한정량추출기를포함한다. 이와같은본 발명에의하면, 배양수조가다각형(8각형) 구조로되어있어, 배양수조들사이의유휴공간에조명장치를설치하여 4면에서광 에너지를제공함으로써, 미세조류의최적성장조건에부합할수 있고, 배양수조의규모확대시 효율적으로공간을활용할수 있는장점이있다.
Abstract translation: 本发明涉及一种用于培养微藻的封闭型光生物反应器。 根据本发明的用于培养微藻类的封闭式光生物反应器包括：具有闭合型结构的培养罐，其中存储微藻，并培养微藻; 照明装置，其安装在培养槽附近，向培养罐提供光能以满足在培养罐中培养的微藻的光照条件; 离心分离器机械地连接到培养罐并离心微藻以收获在培养槽中培养的微藻，并分析微藻的成分; 以及与培养槽机械连接并测定在培养槽中培养的微藻的产生量的定量提取装置。 根据本发明，培养箱具有多边形（八边形）结构，并且照明装置安装在培养罐的各部分之间的空闲空间中，以从四侧提供光能。 因此，封闭型光生物反应器可以为微藻提供最佳生长条件，并且当培养罐的尺寸增加时可以有效地利用空间。
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公开(公告)号：KR101342432B1
公开(公告)日：2013-12-17
申请号：KR1020130027473
申请日：2013-03-14
Applicant: 한국해양과학기술원
CPC classification number: C12P1/00 , C12P17/182 , C12R1/89
Abstract: The present invention relates to a production method of pigment using microalgae. The production method of the pigment using microalgae comprises the following steps: preparing a medium containing 200240 mg/L of NaNO3, 200-240 mg/L of KNO3, 130-200 mg/L of P2O5, 50-100 mg/L of KH2PO4, 20-45 mg/L of MgSO4·7H2O, 25-45 mg/L of Mg(NO3)2·6H2O, 50-100 mg/L of Ca(NO3)2, 25-45 mg/L of K2SO4, 20-45 mg/L of Na2-EDTA·2H2O, 0.5-1.5 mg/L of FeCl3·6H2O, 0.01-1.0 mg/L of Co(NO3)·6H2O, 0.01-1.0 mg/L of MnSO4·5H2O, 0.01-1.0 mg/L of ZnSO4·7H2O, 0.001-0.1 mg/L of CuSO4·5H2O, 0.001-0.1 mg/L of (NH4)6Mo7O24·4H2O, and 0.01-1.0 mL/L of HNO3; adjusting the pH of the medium into 7.50-8.75 using KOH, NaOH, or a mixture thereof; and injecting Haematococcus sp. KORDI03 (requisition number KCTC12348BP) to the medium, and culturing.
Abstract translation: 本发明涉及使用微藻的颜料的制备方法。 使用微藻的颜料的制备方法包括以下步骤：制备含有NaNO 3，200-240mg / L的NaNO 3，200〜240mg / L的KNO 3，130〜200mg / L的P 2 O 5，50〜100mg / L的KH 2 PO 4 ，20-45mg / L的MgSO 4·7H 2 O，25-45mg / L的Mg（NO 3）2·6H 2 O，50-100mg / L的Ca（NO 3）2，25-45mg / L的K 2 SO 4，20 -45mg / L的Na 2 EDTA·2H 2 O，0.5-1.5mg / L的FeCl 3·6H 2 O，0.01〜1.0mg / L的Co（NO 3）·6H 2 O，0.01〜1.0mg / L的MnSO 4·5H 2 O， 1.0mg / L的ZnSO 4·7H 2 O，0.001-0.1mg / L的CuSO 4·5H 2 O，0.001-0.1mg / L的（NH 4）6 Mo 7 O 24·4H 2 O和0.01〜1.0mL / L的HNO 3; 使用KOH，NaOH或其混合物将介质的pH调节至7.50-8.75; 并注射Haematococcus sp。 KORDI03（申请编号KCTC12348BP）培养，培养。
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公开(公告)号：KR1020130126467A
公开(公告)日：2013-11-20
申请号：KR1020130036313
申请日：2013-04-03
Applicant: 한국해양과학기술원
Abstract: The present invention relates to a method for saccharifying marine algae extruded by a high pressure extrusion process by pretreatment and, more particularly, an eco-friendly method for saccharifying marine algae without an acidic or alkaline material by pretreating marine algae through a high pressure extrusion process and a process selected among a microwave process, an ultrasonic process, an ultra-high pressure process, and a homogenization process; and treating the marine algae with saccharifying enzymes. Polysaccharides forming the marine algae, such as cellulose, hemicellulose, starch, and conjugated saccharides which are non-biodegradable polymers are hybridized with high saccharification efficiency by the eco-friendly pretreatment process using water. [Reference numerals] (AA) Marine algae extruded by a high pressure extrusion process;(BB) Microwave process;(CC) Ultrasonic process;(DD) Ultra-high pressure process;(EE) Homogenization process;(FF) Saccharifying enzyme process;(GG) Produce final saccharide
Abstract translation: 本发明涉及一种通过预处理通过高压挤压方法挤出的海藻进行糖化的方法，更具体地说，涉及一种通过高压挤出方法预处理海藻来糖化不含酸性或碱性物质的海藻的环保方法 以及选自微波处理，超声波处理，超高压处理和均质化处理的工序; 并用糖化酶处理海藻。 通过使用水的环保预处理方法，形成海藻类的多糖如纤维素，半纤维素，淀粉和不可生物降解的聚合物的共轭糖与糖化效率高。 （AA）通过高压挤压工艺挤出的海藻;（BB）微波法;（CC）超声波法;（DD）超高压法;（EE）均化法;（FF）糖化酶法 ;（GG）生产最终的糖
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公开(公告)号：KR101233277B1
公开(公告)日：2013-02-14
申请号：KR1020120039981
申请日：2012-04-17
Applicant: 한국해양과학기술원
CPC classification number: C13K1/00 , C10G3/52 , C10G2300/1014 , C12N1/066 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C13K1/02 , Y02E50/16 , Y02E50/17 , Y02P30/20
Abstract: PURPOSE: A method for saccharification of biomass such as marine algae or agricultural by-products is provided to enable hydrolysis of non-degradable polymers with a high saccharifying efficiency. CONSTITUTION: A method for saccharification of marine algae or agricultural by-products comprises: a step of homogenizing and crushing the marine algae or agricultural by-products; and a step of extruding the crushed marine algae or agricultural by-products through a pipe with a diameter of 10-500 um. The marine algae or agricultural by-products are homogenized by rotating at 10,000-50,000 rpm using a homogenizer. The method further comprises a step of extracting the extruded marine algae or agricultural by-products by hot water extraction or high pressure liquefaction and treating with an enzyme. The enzyme is cellulase, amyloglucosidase, beta-agarase, beta-galatosidase, beta-glucosidase, endo-1,4-beta-glucanase, alpha-amylase, or beta-amylase. The marine algae are red algae, brown algae, green algae, or microalgae. [Reference numerals] (AA) Bio mass(seaweed or agricultural byproducts); (BB) Homogenization; (CC) Extrusion(pulverized into nanoparticles); (DD) Hot water extraction; (EE) Extraction by high pressure liquefaction; (FF) Enzymatic saccharification process; (GG) Generating final saccharificated products
Abstract translation: 目的：提供生物质糖化方法，如海藻或农副产品，以使糖化效率高的不可降解聚合物水解。 构成：海藻或农副产品糖化的方法包括：均化和粉碎海藻或农副产品的步骤; 以及通过直径为10-500um的管道挤压破碎的海藻或农副产品的步骤。 海藻或农副产物通过使用均化器以10,000-50,000rpm的转速进行均质化。 该方法还包括通过热水提取或高压液化和用酶处理来提取挤出的海藻或农业副产物的步骤。 酶是纤维素酶，淀粉葡糖苷酶，β-琼脂糖酶，β-半乳糖苷酶，β-葡糖苷酶，内切-1,4-β-葡聚糖酶，α-淀粉酶或β-淀粉酶。 海藻是红藻，褐藻，绿藻或微藻。 （附图标记）（AA）生物质量（海藻或农副产物）; （BB）均质化; （CC）挤出（粉碎成纳米颗粒）; （DD）热水提取; （EE）高压液化提取; （FF）酶法糖化过程; （GG）生产最终的糖化产品
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