公开(公告)号：KR1020120119560A

公开(公告)日：2012-10-31

申请号：KR1020110037569

申请日：2011-04-22

Applicant: 한림대학교 산학협력단

Inventor： 최수영 ,  김대원 ,  박진서 ,  음원식 ,  황현숙 ,  김덕수 ,  한규형 ,  임순성 ,  강태천

IPC: A61K47/42 ,  A61K9/38 ,  C07K19/00 ,  C12N9/02

CPC classification number: A61K47/62 ,  A61K8/64 ,  A61K9/0014 ,  A61K31/48 ,  A61Q19/00 ,  Y10S514/886

Abstract: PURPOSE: An increase method of fused protein into living cells or organs by adding pergolide to fused protein in which protein transport domains and therapeutic protein are covalently bonded is provided to drastically enhance infiltration efficiency of the fused protein. CONSTITUTION: An increase method of fused protein into living cells or organs is processed by adding pergolide to fused protein in which protein transport domains and therapeutic protein are covalently bonded. The pergolide is primarily processed in the living cells or organs than the fused protein. A protein transport domain is Tat, PEP-1, oligolysine, oligo arginine or oligo(lysine and arginine). The pergolide is pergolide mesylate. The therapeutic protein is an enzyme which removes the active oxygen species. The therapeutic protein is catalase, superoxide dismutase or glutathione peroxidase. A composition for preventing and treating disease due to oxidative stress and inflammatory diseases comprises the fused protein and pergolide of the enzyme having the active oxygen species.

Abstract translation: 目的：提供蛋白质转运区域和治疗性蛋白质共价键合的融合蛋白中加入百里香苷后，将融合蛋白增加到活细胞或器官中，大大增强融合蛋白的浸润效率。 构成：通过向蛋白质转运结构域和治疗性蛋白质共价结合的融合蛋白中加入百里高剂来加工融合蛋白进入活细胞或器官的增加方法。 皮质醇主要在活体细胞或器官中处理，而不是融合蛋白。 蛋白质转运结构域是Tat，PEP-1，寡聚赖氨酸，寡聚精氨酸或寡聚（赖氨酸和精氨酸）。 百里香甙是甲磺酸高甘膦。 治疗性蛋白质是去除活性氧的酶。 治疗性蛋白质是过氧化氢酶，超氧化物歧化酶或谷胱甘肽过氧化物酶。 用于预防和治疗由于氧化应激和炎性疾病引起的疾病的组合物包括具有活性氧的酶的融合蛋白和百里香苷。