公开(公告)号：WO03040293A2

公开(公告)日：2003-05-15

申请号：PCT/EP0212137

申请日：2002-10-31

Applicant: BASF AG ,  ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12N

CPC classification number: C07K14/34

Abstract: The invention relates to novel nucleic acid molecules, the use thereof in the construction of bio-engineered improved microorganisms and to methods for the production of fine chemicals, especially amino acids, with the aid of said bio-engineered improved microorganisms.

Abstract translation: 本发明涉及新的核酸分子，它们的遗传改良的微生物以及用于产生精细化学品，在使用这些遗传改良的微生物的特定氨基酸的方法的结构中使用。

公开(公告)号：WO03040181A2

公开(公告)日：2003-05-15

申请号：PCT/EP0212139

申请日：2002-10-31

Applicant: BASF AG ,  ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C07H21/04 ,  C07K14/34 ,  C12N1/21 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12N15/31 ,  C12P13/08 ,  C12R1/15

CPC classification number: C12N9/1223 ,  C07H21/04 ,  C07K14/34 ,  C12N9/16 ,  C12N9/90 ,  C12P13/08 ,  C12R1/15

Abstract: The invention relates to novel nucleic acid molecules, the use thereof in the construction of bio-engineered improved microorganisms and methods for the production of fine chemicals, especially amino acids with the aid of said bio-engineered improved microorganisms.

Abstract translation: 本发明涉及新的核酸分子，它们的遗传改良的微生物以及用于产生精细化学品，在使用这些遗传改良的微生物的特定氨基酸的方法的结构中使用。

公开(公告)号：WO0166573A3

公开(公告)日：2002-05-10

申请号：PCT/IB0002035

申请日：2000-12-22

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR ,  KIM JUN-WON ,  LEE HEUNG-SHICK ,  HWANG BYUNG-JOON

IPC: C12N15/09 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/21 ,  C12N9/90 ,  C12N15/54 ,  C12P20060101 ,  C12P13/08 ,  C12P13/12 ,  C12P21/02 ,  C12R1/13

CPC classification number: C07K14/34 ,  C12N9/90

Abstract: Isolated nucleic acid molecules, designated MP nucleic acid molecules, which encode novel MP proteins form Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MP proteins, mutated MP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of MP genes in this organism.

Abstract translation: 描述编码新型MP蛋白的分离的核酸分子，命名为MP核酸分子，形成谷氨酸棒杆菌。 本发明还提供反义核酸分子，含有MP核酸分子的重组表达载体，以及引入了表达载体的宿主细胞。 本发明还进一步提供分离的MP蛋白，突变的MP蛋白，融合蛋白，抗原肽以及用于改善从C生产所需化合物的方法。 基于该生物体中MP基因的遗传工程的谷氨酸棒杆菌。

公开(公告)号：WO0100805A3

公开(公告)日：2001-10-25

申请号：PCT/IB0000926

申请日：2000-06-23

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: G01N33/53 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/18 ,  C12N15/09 ,  C12N15/31 ,  C12N15/52 ,  C12P20060101 ,  C12P1/04 ,  C12P13/04 ,  C12P21/02 ,  C12Q20060101 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/15 ,  C12R1/16 ,  G01N33/569

CPC classification number: C07K14/34

Abstract: Isolated nucleic acid molecules, designated MCT nucleic acid molecules, which encode novel MCT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MCT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MCT proteins, mutated MCT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of MCT genes in this organism.

Abstract translation: 描述了编码来自谷氨酸棒状杆菌的新型MCT蛋白质的分离的核酸分子，命名为MCT核酸分子。 本发明还提供了反义核酸分子，含有MCT核酸分子的重组表达载体以及导入了表达载体的宿主细胞。 本发明还进一步提供了分离的MCT蛋白，突变的MCT蛋白，融合蛋白，抗原肽和用于改善从C产生所需化合物的方法。 基于该生物体中MCT基因的遗传工程改造的谷氨酸棒杆菌。

公开(公告)号：WO0100804A3

公开(公告)日：2001-08-02

申请号：PCT/IB0000922

申请日：2000-06-23

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR ,  LEE HEUNG-SHICK ,  KIM HYUNG-JOON

IPC: G01N33/53 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/18 ,  C12N15/09 ,  C12N15/31 ,  C12P20060101 ,  C12P1/04 ,  C12P13/04 ,  C12P21/02 ,  C12Q20060101 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/15 ,  C12R1/16 ,  G01N33/569 ,  G01N37/00

CPC classification number: C12N9/00 ,  C07K14/34 ,  C12N9/18

Abstract: Isolated nucleic acid molecules, designated SRT nucleic acid molecules, which encode novel SRT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SRT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SRT proteins, mutated SRT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SRT genes in this organism.

Abstract translation: 描述了分离的核酸分子，命名为SRT核酸分子，其编码来自谷氨酸棒状杆菌的新SRT蛋白质。 本发明还提供了反义核酸分子，含有SRT核酸分子的重组表达载体以及导入了表达载体的宿主细胞。 本发明还进一步提供了分离的SRT蛋白质，突变的SRT蛋白质，融合蛋白质，抗原肽和用于改善从C产生所需化合物的方法。 基于该生物体中SRT基因的遗传工程改造的谷氨酸棒杆菌。

公开(公告)号：WO0100843A2

公开(公告)日：2001-01-04

申请号：PCT/IB0000923

申请日：2000-06-23

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: G01N33/53 ,  C07H21/04 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N9/18 ,  C12N9/90 ,  C12N15/09 ,  C12N15/31 ,  C12N15/61 ,  C12N15/74 ,  C12P20060101 ,  C12P13/04 ,  C12P13/08 ,  C12P21/02 ,  C12P21/06 ,  C12Q20060101 ,  C12Q1/68 ,  C12R1/15 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12N9/18 ,  C07K14/34 ,  C12N9/00 ,  C12N9/1096 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/24 ,  C12Y206/01005

Abstract: Isolated nucleic acid molecules, designated MP nucleic acid molecules, which encode novel MP proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MP proteins, mutated MP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of MP genes in this organism.

Abstract translation: 描述了分离的核酸分子，称为MP核酸分子，其编码来自谷氨酸棒状杆菌的新型MP蛋白。 本发明还提供了反义核酸分子，含有MP核酸分子的重组表达载体以及导入了表达载体的宿主细胞。 本发明还进一步提供了分离的MP蛋白，突变的MP蛋白，融合蛋白，抗原肽和用于改善从C产生所需化合物的方法。 基于该生物体中MP基因的遗传工程的谷氨酸棒杆菌。

公开(公告)号：BRPI0612900A2

公开(公告)日：2014-10-14

申请号：BRPI0612900

申请日：2006-07-18

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  YOCUM R ROGERS ,  ZELDER OSKAR ,  SCHROEDER HARTWIG ,  WILLIAMS MARK K

IPC: C12P13/12

Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &Dgr;metF organism or a &Dgr;metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &Dgr;metF &Dgr;metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

公开(公告)号：BRPI0520842A2

公开(公告)日：2012-07-03

申请号：BRPI0520842

申请日：2005-12-21

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA

IPC: C12N15/77 ,  C07K14/34

Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

公开(公告)号：IN387CH2008A

公开(公告)日：2008-09-19

申请号：IN387CH2008

申请日：2008-01-23

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  KIEFER PATRICK ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/16 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：TR200707458T2

公开(公告)日：2008-01-21

申请号：TR200707458

申请日：2000-06-23

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  HABERHAUER GREGOR ,  ZELDER OSKAR ,  SCHROEDER HARTWIG

IPC: C12N9/00 ,  G01N33/53 ,  C07K14/34 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12N9/18 ,  C12N9/88 ,  C12N15/09 ,  C12N15/31 ,  C12N15/55 ,  C12P13/04 ,  C12P13/08 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/15 ,  G01N33/566 ,  G01N33/569