公开(公告)号：WO1992008123A1

公开(公告)日：1992-05-14

申请号：PCT/US1991008118

申请日：1991-10-30

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  MORRIS, Roger, James ,  BASCOMB, Shoshana ,  OLSON, Carolyn, Shelchuk

IPC: G01N21/78

CPC classification number: G01N21/17 ,  G01N21/534 ,  G01N21/78 ,  G01N2201/122 ,  Y10S436/80

Abstract: The present invention relates to a process to determine the concentration of any substance in a colorimetric, turbidimetric or nephelometric reaction using a fluorometric detector to measure fluorescence intensity. In particular, a change in color can be monitored by observing the measurement of fluorescence intensity of a fluorophore in an inert matrix. The absorption spectrum of the chromophore may overlap the excitation and/or emission spectrum of the fluorophore, thereby allowing the change in fluoroscence to be related to the intensity of color in the reaction and thus related to the quantity of the substance of interest.

Abstract translation: 本发明涉及使用荧光检测器测定荧光强度的比色法，比浊法或比浊法反应中任何物质的浓度的方法。 特别地，可以通过观察惰性基质中荧光团的荧光强度的测量来监测颜色变化。 发色团的吸收光谱可能与荧光团的激发和/或发射光谱重叠，从而允许荧光的变化与反应中的颜色强度相关，并且因此与感兴趣的物质的量有关。

公开(公告)号：WO1991019814A2

公开(公告)日：1991-12-26

申请号：PCT/US1991004190

申请日：1991-06-12

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  PICKEN, Roger, N. ,  AMMONS, Harryl, C.

IPC: C12Q01/68

CPC classification number: C07K14/20 ,  C12Q1/689 ,  Y02A50/57

Abstract: Nucleic acid primers of complementary sequence to portions of the gene encoding a semiconserved region of B. burgdorferi flagellin are utilized to amplify target sequences of nucleic acids extracted from infective sites of Lyme borreliosis, which are then probed with a nucleic acid tracer conjugated to signal-generating molecules for highly specific detection of B. burgdorferi.

Abstract translation: 使用与编码布氏疏螺旋体鞭毛蛋白半数位区域的基因的部分互补序列的核酸引物来扩增从Lyme borreliosis感染位点提取的核酸的靶序列，然后用缀合至信号通路的核酸示踪剂探测， 产生分子，用于高度特异性检测布氏疏螺旋体。

公开(公告)号：WO1991017240A1

公开(公告)日：1991-11-14

申请号：PCT/US1991003010

申请日：1991-05-02

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  YEN-MAGUIRE, Yu, Ping ,  COX, Tom ,  LEWIS, Fred

IPC: C12M03/00

CPC classification number: G01N33/5011 ,  C12M23/12 ,  C12M41/46 ,  G01N2800/52

Abstract: A method and apparatus for assaying the sensitivity of biopsied tumor cells to chemotherapeutic agents using a predetermined amount of chemotherapeutic agent in an easily deliverable form is disclosed and claimed.

Abstract translation: 公开和要求保护的方法和装置，用于使用预定量的化学治疗剂以易于递送的形式测定活检肿瘤细胞对化学治疗剂的敏感性。

公开(公告)号：WO1991013169A1

公开(公告)日：1991-09-05

申请号：PCT/US1991001367

申请日：1991-02-27

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  GRUYS, Kenneth, J. ,  AMMONS, Harryl, C.

IPC: C12Q01/32

CPC classification number: C12Q1/52 ,  C12Q1/32 ,  C12Q1/48

Abstract: A method to use a reaction by-product as a calibrator in an automated enzymatic assay is described. In particular, the reaction by-product pyruvate is used as an instrument calibration standard to measure the activity of alanine aminotransferase. Use of this method eliminates errors due to inaccurate pipetting and corrects for small optical deviations from the wavelength of interest in the instrument. Left uncorrected, pipetting error and optical deviation negatively impact the accuracy of the enzyme activity calculation. In the fluorescence mode, calibration with a pyruvate standard also eliminates having to use unstable NADH standards. The pyruvate calibration standard of this invention is shown to be stable in standard stability testing. This method can be extended to a number of other automated enzymatic assays. Like alanine aminotransferase, the accuracy of the activity calculation for other enzymes should be increased by use of this procedure.

Abstract translation: 描述了在自动化酶测定中使用反应副产物作为校准物的方法。 特别地，反应副产物丙酮酸被用作仪器校准标准来测量丙氨酸氨基转移酶的活性。 使用此方法消除了由于不准确的移液造成的错误，并纠正了与仪器感兴趣的波长有关的小光学偏差。 左未校正，移液误差和光学偏差对酶活性计算的准确性产生负面影响。 在荧光模式下，用丙酮酸标准品进行校准也不必使用不稳定的NADH标准品。 本发明的丙酮酸校准标准在标准稳定性测试中显示为稳定的。 该方法可以扩展到许多其他自动化酶测定。 如丙氨酸氨基转移酶，其他酶活性计算的准确性应通过使用该程序来增加。

公开(公告)号：WO1995012818A1

公开(公告)日：1995-05-11

申请号：PCT/US1994012693

申请日：1994-11-04

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  LEE, Ted, C., K. ,  PELZER, Franz, Hermann ,  MOTLEY, Leslie, A.

IPC: G01N33/86

CPC classification number: G01N33/86 ,  Y10S514/834 ,  Y10T436/106664

Abstract: The compound 2,3,5,6-tetrahydroxy-1,4-quinone, its derivatives and structural analogs are used as activators for intrinsic blood coagulation and as diagnostic reagents for the activated partial thromboplastin time test of blood coagulation.

Abstract translation: 化合物2,3,5,6-四羟基-1,4-醌，其衍生物和结构类似物用作固有凝血活化剂，并用作凝血活化部分凝血活酶时间试验的诊断试剂。

公开(公告)号：WO1995008771A1

公开(公告)日：1995-03-30

申请号：PCT/US1994010032

申请日：1994-09-07

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  LIN, Spencer, H. ,  SINGH, Pratap ,  DIAMOND, Steven, E.

IPC: G01N33/543

CPC classification number: C07K16/3007 ,  C07K16/26 ,  C07K16/40 ,  G01N33/54353

Abstract: Methods and compositions are provided for specific binding assays in which specific binding reagents are immobilized on a solid phase. Immobilization is facilitated by coupling bifunctional reagents to specific binding assay proteins thereby chemically cross-linking the specific binding assay reagents. Such cross-linked specific binding assay reagents provide the advantage of performing assays without the use of secondary reagents. This provides for enhanced sensitivity through minimizing nonspecific background interference with the solid phase or with the target samples.

Abstract translation: 提供了用于特异性结合试验的方法和组合物，其中特异性结合试剂固定在固相上。 通过将双功能试剂偶联到特异性结合测定蛋白，从而化学交联特异性结合测定试剂来促进固定化。 这种交联特异性结合测定试剂提供了在不使用第二试剂的情况下进行测定的优点。 这通过最小化与固相或目标样品的非特异性背景干扰来提供增强的灵敏度。

公开(公告)号：WO1995001839A1

公开(公告)日：1995-01-19

申请号：PCT/US1994007507

申请日：1994-07-06

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  JONES, Donald

IPC: B01L11/00

CPC classification number: B01L3/0293 ,  Y10T137/2579 ,  Y10T137/2642

Abstract: A precision liquid reagent fluid dispensing apparatus (10) and method includes a continuously operating pump (16) inducting and pressurizing ambient air. The pressurized air is delivered at a first location into a flow-through volume (18) with provision to substantially completely abate at a second location any noncontinuous air flow or air pressure variations from the pump. A long-lived pressure sensor (32) which is substantially free of drift is associated with the flow through volume at a third location and provides a signal indicative of sensed air pressure. A controller (36) by reference to a calibration standard for the sensor provides a time-variant control signal in response to the sensed air pressure. At a fourth location a duty cycle valve (38) vents pressurized air from the flow through volume in response to the control signal to provide at the second location a precisely regulated dispensing air pressure. The dispensing air pressure is applied via a reagent liquid container (46) and closure member (62) to a liquid reagent to pressurize this reagent for dispensing at a precise rate via a dispensing valve (82). The dispensing valve is sealingly carried by the closure member to reduce leakage paths and corrosion opportunities in the apparatus of the present invention.

Abstract translation: 精密液体试剂流体分配装置（10）和方法包括对环境空气进行感应和加压的连续运行的泵（16）。 加压空气在第一位置处被输送到流通体积（18）中，其设置成在第二位置基本上完全减轻与泵不连续的气流或气压变化。 基本上没有漂移的长寿命压力传感器（32）与在第三位置处的流量相关联并且提供指示感测到的空气压力的信号。 参考传感器的校准标准的控制器（36）响应于感测到的空气压力而提供时变控制信号。 在第四位置，响应于控制信号，占空比阀（38）从加压空气排出流量，以在第二位置提供精确调节的分配空气压力。 分配空气压力经由试剂液体容器（46）和封闭构件（62）经由分配阀（82）以精确的速率施加到液体试剂以加压该试剂。 分配阀由封闭构件密封地承载，以减少本发明装置中的泄漏路径和腐蚀机会。

公开(公告)号：WO1994024569A1

公开(公告)日：1994-10-27

申请号：PCT/US1994003884

申请日：1994-04-08

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  HERRING, Kathryn ,  SANDBERG, Denise

IPC: G01N33/96

CPC classification number: G01N33/96 ,  G01N33/574 ,  G01N33/57488 ,  G01N2333/695 ,  G01N2333/705 ,  G01N2333/82

Abstract: The present invention provides a stable control serum or plasma for tumor diagnosis, wherein the control contains the tumor markers relevant for the diagnosis of tumors. The serum or plasma has a reduced lipid content. The present invention also provides for a method of making the control.

Abstract translation: 本发明提供用于肿瘤诊断的稳定的对照血清或血浆，其中所述对照含有与肿瘤诊断相关的肿瘤标志物。 血清或血浆脂质含量降低。 本发明还提供了一种进行控制的方法。

公开(公告)号：WO1994019691A1

公开(公告)日：1994-09-01

申请号：PCT/US1994001906

申请日：1994-02-14

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  SINGH, Pratap ,  JOHNSON, Randy, W.

IPC: G01N33/53

CPC classification number: C12Q1/42 ,  G01N33/54393 ,  G01N33/581

Abstract: A composition and method are disclosed to reduce the effects that endogenous alkaline phosphatase found in some human bodily fluid samples have on solid phase immunoassay systems. The composition comprises a detergent, inhibitors to alkaline phosphatase, and a buffer and may contain a substrate to alkaline phosphatase.

Abstract translation: 公开了一种组合物和方法，以减少在一些人体液样品中发现的内源性碱性磷酸酶对固相免疫测定系统的影响。 组合物包含洗涤剂，碱性磷酸酶抑制剂和缓冲液，并且可以含有碱性磷酸酶的底物。

公开(公告)号：WO1994008716A1

公开(公告)日：1994-04-28

申请号：PCT/US1993009395

申请日：1993-10-04

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  HUNTLEY, Brent ,  WOODING, Michael ,  GODSEY, James

IPC: B01L03/00

CPC classification number: B01L3/50255 ,  B01L9/523 ,  B01L2300/0829

Abstract: A cartridge for holding an analytical sample during a diagnostic assay comprises a panel with a number of adjacent wells in its upper side. The wells have an upper opening and a lower opening, and the lower opening extends to the underside of the panel. A filter membrane extends across the lower openings of the wells so that liquids contained in the wells may be drawn through the filter, thereby concentrating suspended solids near the lower openings of the wells. The panel includes a supporting wall for supporting the panel on a non-integral, external surface, which does not form a part of the panel.

Abstract translation: 在诊断测定期间用于保持分析样品的筒包括在其上侧具有多个相邻孔的面板。 井具有上开口和下开口，并且下开口延伸到面板的下侧。 过滤膜延伸穿过孔的下部开口，使得包含在孔中的液体可以被抽吸通过过滤器，从而将靠近孔的下部开口的悬浮固体集中。 面板包括用于将面板支撑在非整体的外表面上的支撑壁，其不形成面板的一部分。