中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

175 records (took 0.022 seconds)

    Cell Culture System
    42.
    发明专利
    Cell Culture System 未知

    公开(公告)号：SG10201601434RA

    公开(公告)日：2016-03-30

    申请号：SG10201601434R

    申请日：2012-02-28

    Applicant: HARVARD COLLEGE

    Inventor： INGBER DONALD E ,  KIM HYUN JUNG

    Abstract: A method of producing a co-culture of intestinal epithelial cells and microbial cells, comprising providing a cell culture system comprising a fluidic device having a fluid channel connected to a fluid source, the fluid source supplying fluid to the fluid channel; and a membrane positioned within the channel. The method further comprises providing a fluid suitable for maintaining intestinal epithelial cells to the cell culture system such that the fluid contacts the intestinal epithelial cells, culturing the intestinal epithelial cellswherein the intestinal epithelial cells adhere to at least one surface of the membrane, maintaining microbial cells in the system for at least 1 day, wherein the microbial cells adhere to the surface of the viable intestinal epithelial cells, and flowing fluid continuously through the channel such that microbial cells not adhering to the intestinal epithelial cells are washed out.

    Systems and methods for cell culture device interconnection and fluidic device interconnection
    43.
    发明专利
    Systems and methods for cell culture device interconnection and fluidic device interconnection 未知

    公开(公告)号：AU2014287013A1

    公开(公告)日：2016-02-04

    申请号：AU2014287013

    申请日：2014-07-11

    Applicant: HARVARD COLLEGE

    Inventor： INGBER DONALD E ,  LEVNER DANIEL ,  THOMPSON III GUY ,  FERNANDEZ-ALCON JOSE ,  HINOJOSA CHRISTOPHER DAVID

    IPC: C12M3/00 ,  B01L3/02 ,  C12M1/26

    Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

    Engineered microbe-targeting molecules and uses thereof
    45.
    发明专利
    Engineered microbe-targeting molecules and uses thereof 未知

    公开(公告)号：AU2012284097A1

    公开(公告)日：2014-02-06

    申请号：AU2012284097

    申请日：2012-07-18

    Applicant: HARVARD COLLEGE

    Inventor： INGBER DONALD E ,  SUPER MICHAEL ,  WAY JEFFREY CHARLES ,  CARTWRIGHT MARK J ,  WATTERS ALEXANDER ,  BERTHET JULIA B ,  SUPER DINAH R ,  ROTTMAN MARTIN M

    IPC: C07K14/47 ,  A61K47/48 ,  C07K14/705 ,  G01N33/543 ,  G01N33/569

    Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

    DIALYSIS LIKE THERAPEUTIC (DLT) DEVICE
    49.
    发明专利
    DIALYSIS LIKE THERAPEUTIC (DLT) DEVICE 未知

    公开(公告)号：CA2831857A1

    公开(公告)日：2012-10-04

    申请号：CA2831857

    申请日：2012-04-02

    Applicant: CHILDRENS MEDICAL CENTER ,  HARVARD COLLEGE

    Inventor： YUNG CHONG WING ,  DOMANSKY KAREL ,  TERRY RICHARD C ,  KALISH DAVID ,  SCHULTE ALEXA ,  KANG JOO HUN ,  INGBER DONALD E ,  SUPER MICHAEL ,  COOPER RYAN M

    IPC: G01N33/569 ,  B81B1/00 ,  G01N35/08 ,  G01N35/10

    Abstract: A dialysis like therapeutic (DLT) device is provided. The DLT device includes at least one source channel connected at least one collection channels by one or more transfer channels. Fluid contacting surface of the channels can be an anti-fouling surface such as slippery liquid-infused porous surface (SLIPS). Fluids can be flown at high flow rates through the channels. The target components of the source fluid can be magnetic or bound to magnetic particles using an affinity molecule. A source fluid containing magnetically bound target components can be pumped through the source channel of the microfluidic device. A magnetic field gradient can be applied to the source fluid in the source channel causing the magnetically bound target components to migrate through the transfer channel into the collection channel. The collection channel can include a collection fluid to flush the target components out of the collection channel. The target components can be subsequently analyzed for detection and diagnosis. The source channel and the collection channels of the microfluidic device are analogous to the splenic arterioles and venules, respectively; the transfer channels mimic the vascular sinusoids of the spleen where opsonized particles are retained. Thus, the device acts as a dialysis like therapeutic device by combining fluidics and magnetics.

    ENGINEERED OPSONIN FOR PATHOGEN DETECTION AND TREATMENT
    50.
    发明专利
    ENGINEERED OPSONIN FOR PATHOGEN DETECTION AND TREATMENT 未知

    公开(公告)号：SG182577A1

    公开(公告)日：2012-08-30

    申请号：SG2012052965

    申请日：2011-01-19

    Applicant: HARVARD COLLEGE

    Inventor： SUPER MICHAEL ,  WAY JEFFREY CHARLES ,  INGBER DONALD E

    Abstract: The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.

Patent Agency Ranking