Abstract:
Apparatus is provided for the detection of chemiluminescence, e.g., the luminescent assay of an analyte in a sample. Sample, namely the production of a permanent photographic record of such chemiluminescence, e.g., by the use of a POLAROID film. The apparatus includes three interrelated elements. The first element comprises a film holder for holding a photographic film sensitive to a chemiluminescent reaction of the sample, the film holder defining a window in registry with any photographic film which may be held in the film holder. The second element comprises a sample holder for holding the sample in registry with the window and any photographic film which may be held in the film holder, and for allowing movements of the sample relative to any photographic film which may be held in the film holder between (i) a raised position, wherein the sample holder, and thus the sample is in spaced relation to any photographic film which may be held in the film holder and (ii) a lowered position, wherein said sample holder is in sufficiently close proximity to any photogarphic film which may be held in the film holder means to allow the sample held thereby directly to contact any photographic film which may be held in the film holder. The third element comprises a shutter operatively associated with the window of the film holder and movable between a closed position, wherein the shutter obstructs the window so as to prevent the sample holder from moving into the lowered position, and an opened position, wherein the window is unobstructed by the shutter so as to permit the sample holder to move from the raised position to the lowered position. The production of the permanent photographic record begins when the test sample in the container is placed in direct contact with the photographic film. The production of the photographic record is stopped when the test sample in the container is removed out of direct contact with the photographic film and the slidig shutter is closed. Various test results can be observed by varying the time of direct contact of the test sample in the reactant container with the photographic film. This provides maximal analytical sensitivity. During the entire test, exposure to ambient light is prevented.
Abstract:
A multi-chronal fluorescence imaging technique for spatial differentiation and correlation of a plurality of separate sample components tagged with site specific dyes is described. The dyes have decay times that are widely separated and vary by approximately a factor of ten from one dye to the next. These are added to the sample and are excited with a short pulse of ultraviolet light. Between the sample and a detector is placed an adjustable shutter or gate which is opened and closed at predetermined intervals so that the detector can see the approximate maximum intensity output of each dye in a shown ordered sequence without significant interference from the other dyes.
Abstract:
The present disclosure, among other things, describes a reader system comprising a casing, an optical system, en electromechanical motor system, and one or more digital processors.
Abstract:
A method and apparatus for visually detecting when a luminescent dissolved oxygen sensor is operating is disclosed. In one example embodiment of the invention, a shutter (216) is placed into the light tight container. When the shutter (216) is open, a user can see into the light tight container and verify probe operation. When the shutter (216) is closed, external light is prevented from entering the light tight container and affecting measurement accuracy. In another example embodiment of the invention, one end of a light pipe (526) is placed on the outside of the light tight container, and the other end is positioned to view the light source (504) of the probe. In another example embodiment of the invention a second light source (628), visible on the outside of the light tight container, is used to verify operation of the probe. In another example embodiment of the invention, a predetermined area is left open in the optically opaque hydrostatically transparent (814) on the face of the sensor window, allowing a user to see light from the sensor when the sensor is operating properly.
Abstract translation:公开了一种用于可视地检测发光溶解氧传感器何时在工作的方法和装置。 在本发明的一个示例性实施例中,快门(216)被放置在不透光的容器中。 当快门(216)打开时,用户可以看到不透光的容器并验证探针操作。 当快门(216)关闭时,防止外部光进入不透光容器并影响测量精度。 在本发明的另一示例性实施例中,光管(526)的一端放置在不透光的容器的外侧,另一端定位成观察探针的光源(504)。 在本发明的另一示例性实施例中,在不透光容器的外侧上可见的第二光源(628)被用于验证探针的操作。 在本发明的另一示例性实施例中,预定区域在传感器窗口的面上的光学不透明流体透明(814)中保持打开,使得用户在传感器正常运行时看到来自传感器的光。 p >
Abstract:
A sample holder and shutter assembly (20) for photometers in which a stationary sample chamber body (40) is mounted in close proximity to a photosensitive device (18). A light path is provided from the sample chamber (53) to the photosensitive device (18) and shutter means is provided for selectively interrupting and opening the light path between the photosensitive device (18) and the sample chamber (53) without moving the sample chamber. Heat exchange means including a heat pump (63) and a heat reservoir (68) are provided for maintaining a desired temperature in the sample chamber (53). The sample chamber body (40) is thermally connected to said heat pump (63) and is preferably thermally isolated from the means mounting it. In one embodiment, the heat pump (63) and heat reservoir (68) are flexibly connected.
Abstract:
L'invention se rapporte à un système d'observation (15) d'une plaque (10) comportant des puits (20), comportant, pour chaque puits (20): - une source (40) comprenant une diode électroluminescente (60) propre à produire un rayonnement lumineux, un sténopé (70), et un intégrateur de lumière (65) - un capteur optique (185) propre à récolter le signal optique issu du puits (20), le système (15) étant tel que : - un rapport entre la longueur et la dimension transversale moyenne (Dt) de chaque intégrateur de lumière (65) est supérieur ou égal à 2,2, ou - au moins un axe optique est décentré par rapport à la ligne de propagation, le rapport entre la longueur et la dimension transversale moyenne de l'intégrateur étant supérieur ou égal à 1,5.
Abstract:
A diagnostic analyzer includes a track, a light-blocking member, a motor, and an optical testing device. The track moves a reaction vessel held by the track. The light-blocking member is disposed adjacent to the track. The light-blocking member moves from a first position apart from the track to a second position closer to the track. When the light-blocking member is disposed in the first position a sample contained within the reaction vessel held by the track is exposed to light. When the light-blocking member is disposed in the second position the sample contained within the reaction vessel held by the track is blocked from exposure to the light. The motor moves the light-blocking member between the first and the second positions. The optical testing device is disposed adjacent to the track for optically testing the sample contained within the reaction vessel held by the track when the at least one light- blocking member is disposed in the second position.
Abstract:
The present invention relates to an apparatus and method for imaging time resolved fluorescence in biochemical and medical samples. In a primary aspect, the device includes a lens of large aperture, a flash lamp in the illumination path, a fast-acting solid state shutter or a gated detector in the emission path, a device for delivering homogenous monochromatic illumination to a plurality of wells distributed within a microwell plate, a digital camera of high quantum efficiency, and a computer. under computer control, the lamp is pulsed at short intervals. The fast-acting emission shutter or gated detector operates to limit exposure of the camera to a period some microseconds after the extinction of each lamp pulse, during which only delayed fluorescence is transmitted to the camera. The invention achieves simultaneous time resolved imaging of a plurality of samples, with high sensitivity and high throughput.
Abstract:
A diagnostic analyzer includes a track, a light-blocking member, a motor, and an optical testing device. The track moves a reaction vessel held by the track. The light-blocking member is disposed adjacent to the track. The light-blocking member moves from a first position apart from the track to a second position closer to the track. When the light-blocking member is disposed in the first position a sample contained within the reaction vessel held by the track is exposed to light. When the light-blocking member is disposed in the second position the sample contained within the reaction vessel held by the track is blocked from exposure to the light. The motor moves the light-blocking member between the first and the second positions. The optical testing device is disposed adjacent to the track for optically testing the sample contained within the reaction vessel.