公开(公告)号：WO1996022524A1

公开(公告)日：1996-07-25

申请号：PCT/US1995016940

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KEO, Nida ,  SAFARIAN, Zara ,  LIU, Cheng-Ming ,  WANG, HANN-Ping

IPC: G01N27/447

CPC classification number: G01N27/44747

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Abstract translation: 用于通过毛细管区带电泳分析糖蛋白的缓冲液和方法。 缓冲液包括水，糖络合化合物，用于调节pH的碱性化合物和两性离子化合物。 缓冲剂的一个实施方案包括作为络合化合物的硼酸钠，作为碱的氢氧化钠和作为两性离子化合物的3-环己基氨基-1-丙磺酸。 在该方法中，将选择的糖蛋白进行毛细管区带电泳。 通过对所得电泳图的定量分析来确定糖蛋白的比例或量。

公开(公告)号：WO1996006174A1

公开(公告)日：1996-02-29

申请号：PCT/US1994009450

申请日：1994-08-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W. ,  PANG, Patty, P., Y. ,  BELEI, Marina, C.

IPC: C12N15/55

CPC classification number: C12N9/22 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/02

Abstract: The gene for Streptococcus pyogenes DNase B has been cloned and vectors incorporating the cloned DNA have been used to transform Escherichia coli, allowing efficient and rapid production of the DNase in E. coli without the necessity of growing large quantities of S. pyogenes. The enzyme can be produced with aleader peptide at its amino terminus. An improved method for the purification of naturally occurring S. pyogenes DNase B enzyme is also provided. The DNase B enzyme produced, either by purification of naturally occurring enzyme or by recombinant DNA techniques, can be used to generate antibodies and can also be used in immunochemical assays to detect the presence of anti-DNase B antibodies in serum as a marker of infection by S. pyogenes.

Abstract translation: 已经克隆了化脓性链球菌DNase B的基因，并且已经使用掺入克隆DNA的载体来转化大肠杆菌，从而能够在大肠杆菌中高效快速地生产DNA酶，而不需要生长大量的化脓性链球菌。 该酶可以在其氨基末端用引入肽产生。 还提供了用于纯化天然存在的化脓性链球菌DNA酶B酶的改进方法。 通过纯化天然存在的酶或通过重组DNA技术产生的DNA酶B酶可用于产生抗体，也可用于免疫化学测定以检测血清中抗DNase B抗体作为感染标志物的存在 化脓性链球菌。

公开(公告)号：WO1996005500A1

公开(公告)日：1996-02-22

申请号：PCT/US1995009926

申请日：1995-08-03

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OBREMSKI, Robert, J. ,  SILZEL, John, W.

IPC: G01N21/64

CPC classification number: G01N21/314 ,  G01J3/4338 ,  G01N21/64 ,  G01N2201/1293

Abstract: A method and apparatus determines the analyte content of a sample by generating first and second input signals and directing the input signals to the sample. The input signals differ in wavelength by at least 3 nanometers. Due to the interaction between the input signals and the sample, first and second output signals are generated. Each output signal comprises a resonant signal whose peak wavelength is substantially independent of the wavelength of the respective input signal, and a non-resonant output signal whose peak wavelength is dependent upon the wavelength of respective input signal. A detector is used to detect the two output signals, and by distinguishing the resonant output signals from the non-resonant output signals, data about the analyte content of the sample is determined. Principal components regression analysis or multivariate quantitative analysis can be applied to the output signals, for the purpose of distinguishing between the resonant and non-resonant signals. The method and apparatus can also distinguish resonant output signals from each other, and non-resonant output signals from each other.

Abstract translation: 方法和装置通过产生第一和第二输入信号并将输入信号引导到样品来确定样品的分析物含量。 输入信号波长不同至少3纳米。 由于输入信号和采样之间的相互作用，产生了第一和第二输出信号。 每个输出信号包括峰值波长基本上与各个输入信号的波长无关的谐振信号，以及峰值波长取决于相应输入信号的波长的非谐振输出信号。 检测器用于检测两个输出信号，并且通过区分谐振输出信号与非谐振输出信号，确定关于样品的分析物含量的数据。 主成分回归分析或多变量定量分析可以应用于输出信号，以区分谐振和非谐振信号。 该方法和装置还可以区分彼此的谐振输出信号和彼此之间的非谐振输出信号。

公开(公告)号：WO1996004555A1

公开(公告)日：1996-02-15

申请号：PCT/US1995009476

申请日：1995-07-27

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHENG, Anthony, K. ,  KIM, Julie, S. ,  OH, Chan, S.

IPC: G01N33/53

CPC classification number: G01N33/54393 ,  Y10S435/962 ,  Y10S436/805 ,  Y10S436/817 ,  Y10S436/826 ,  Y10S436/909

Abstract: Methods for modulating the rates and dose responses of immunoassays through the incorporation of one or more detergents into the immunoassay reaction are disclosed. The methods are particularly suitable for automated immunoassay formats, especially with formats that use analyte-biotin bidentate reagents. The methods may be used to facilitate the detection of any desired, preselected pharmacological agent.

Abstract translation: 公开了通过将一种或多种洗涤剂并入免疫测定反应来调节免疫测定的速率和剂量反应的方法。 该方法特别适用于自动化免疫分析方法，特别是使用分析物 - 生物素二齿试剂的方法。 所述方法可用于促进检测任何所需的预选药理剂。

公开(公告)号：WO1996001697A1

公开(公告)日：1996-01-25

申请号：PCT/US1995008557

申请日：1995-07-07

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SRINIVASAN, Krupa, S.

IPC: B04B13/00

CPC classification number: B04B13/003

Abstract: A centrifuge system (10) and method includes generating a radio frequency excitation field within a housing (32) containing a rotor (16) of interest. The excitation field may be generated by an exciter coil (37) fixed to the cover (34) of the housing. The rotor includes a locking knob (23) that encloses a receiver coil (25) inductively coupled to the exciter coil. The excitation field causes current flow through the receiver coil. The current is rectified and used to power encoding circuitry. The encoding circuitry produces a modulated signal unique to the rotor or to a model in which the rotor is classified. The encoded signal is transmitted from within the locking knob to a reader coil (34) connected to the housing of the centrifuge. The reader coil receives the encoded signal, whereafter the signal is decoded and used to identify the rotor or rotor model.

Abstract translation: 离心机系统（10）和方法包括在包含感兴趣的转子（16）的壳体（32）内产生射频激励场。 励磁场可以由固定到壳体的盖（34）的激励器线圈（37）产生。 转子包括锁定旋钮（23），该锁定旋钮包围感应地耦合到激励器线圈的接收器线圈（25）。 励磁场引起电流流过接收器线圈。 电流被整流并用于对编码电路供电。 编码电路产生对转子独特的调制信号或转子分类的模型。 编码信号从锁定旋钮内传送到连接到离心机壳体的读取器线圈（34）。 读取器线圈接收编码信号，此后信号被解码并用于识别转子或转子模型。

公开(公告)号：WO1996001695A1

公开(公告)日：1996-01-25

申请号：PCT/US1995008507

申请日：1995-07-07

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SCHENCK, Alan, L. ,  SONG, Jin, Y.

IPC: B04B13/00

CPC classification number: B04B15/08 ,  B04B13/003 ,  B04B15/02 ,  G01N2001/4027 ,  G01N2035/00495

Abstract: A method and system of determining an operation parameter for a centrifuge includes measuring one or more physical characteristics of a rotor interchangeably supported by a rotor drive. In the preferred embodiment, windage is measured at a first rotational speed and a speed-dependent signal is generated to adjust a vacuum system based upon changes in rotational speed of the rotor. Changes in windage may also be used as a basis for adjusting a refrigeration circuit. Either as an alternative or in addition to monitoring windage, the moment of inertia can be measured and used to adjust adaptive circuitry. For example, the drive circuitry may be adjusted based upon a determination of inertia as the physical characteristic of interest. The method and system isolate inertia and windage as the main forms of resistance to acceleration of the rotor.

Abstract translation: 确定离心机的操作参数的方法和系统包括测量由转子驱动器可互换地支撑的转子的一个或多个物理特性。 在优选实施例中，以第一旋转速度测量风速，并且产生基于速度的信号，以基于转子的转速的变化来调节真空系统。 风阻变化也可以用作调节制冷回路的基础。 作为监测风向的替代或补充，惯性矩可以被测量并用于调整自适应电路。 例如，可以基于惯性的确定来调整驱动电路作为感兴趣的物理特性。 该方法和系统将惯性和风阻作为转子加速阻力的主要形式。

公开(公告)号：WO1995032428A1

公开(公告)日：1995-11-30

申请号：PCT/US1995006367

申请日：1995-05-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  YAN, Cheng, F. ,  OH, Chan, S. ,  CHENG, Anthony, K.

IPC: G01N33/543

CPC classification number: G01N33/532 ,  G01N33/58 ,  G01N33/94 ,  Y10S436/805 ,  Y10S436/815 ,  Y10S436/816

Abstract: Bidentate reagents for rapidly and quantitatively assaying the concentration of pharmacological agents in biological samples are described. The reagents are used in an immunoassay format for determining the concentration of desired, preselected pharmacological agents, such as benzoylecgonine, cocaine, an opiate, PCP, digoxigenin, acetaminophen, carbamazepine, phenytoin, primidone, theophylline, an aminoglycoside antibiotic, vancomycin, quinidine or a cannabinoid.

Abstract translation: 描述了用于快速和定量测定生物样品中药理学剂浓度的二齿试剂。 试剂以免疫测定方式用于测定所需预选的药理学试剂如苯甲酰扁桃酸，可卡因，阿片剂，PCP，地高辛，对乙酰氨基酚，卡马西平，苯妥英，扑米酮，茶碱，氨基糖苷类抗生素，万古霉素，奎尼丁或奎宁 一种大麻素。

公开(公告)号：WO9521270A3

公开(公告)日：1995-10-19

申请号：PCT/US9501468

申请日：1995-02-03

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/686 ,  C12Q1/6862

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

公开(公告)号：WO1995021270A2

公开(公告)日：1995-08-10

申请号：PCT/US1995001468

申请日：1995-02-03

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W.

IPC: C12Q01/68

CPC classification number: C12Q1/686 ,  C12Q1/6862

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

Abstract translation: 公开了靶核酸分子的检测和指数扩增的方法和试剂。 该方法通常使用引物寡核苷酸，其与目标分子的链上的阻断剂寡核苷酸和可以与阻断剂寡核苷酸杂交的末端寡核苷酸一起杂交。

公开(公告)号：WO1995011748A1

公开(公告)日：1995-05-04

申请号：PCT/US1994012443

申请日：1994-10-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MATSON, Robert, S. ,  COASSIN, Peter, J. ,  RAMPAL, Jang, B. ,  SOUTHERN, Edwin, M.

IPC: B01J19/00

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0043 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00722 ,  C07K1/045 ,  C12Q1/6874 ,  C40B40/06 ,  C40B60/14 ,  G01N35/1072

Abstract: An automated method and apparatus for performing biopolymer synthesis on a two-dimensional support surface (56) whereby a two-dimensional matrix or array of biopolymers are obtained on the surface. An applicator (10) in the form of a thick plate (12) having in a surface (16) at least one cavity in the form of an open chamber or channel (14) is used for applying reagents to the surface (56) of a solid phase support material. The applicator (10) is positioned over the solid support material (56) with the surface having the cavity (14) sealed against the material surface. For each cavity (14), reagents for synthesis are introduced sequentially into one end of the cavity and collected from the other end of the cavity. A one-dimensional array of biopolymers are thus formed on the support material (56), where each element of the array contains a population of biopolymers having identical sequence. Further synthesis of additional biopolymers is performed by linearly moving or rotating the applicator (10) relative to the support material (56) and carrying out synthesis procedure so that another one-dimensional matrix or array of biopolymers are formed at an offset position to the previous array. At each overlapping region of the two arrays, a cell of biopolymers are formed which each comprises a strand from the first array and a strand from the second array. The result is a two-dimensional array of biopolymers of different sequences at each discrete cell. The arrays of discrete cell containing different biopolymers may be used to conduct hybridization reaction analysis of a biological sample.

Abstract translation: 一种用于在二维支撑表面（56）上进行生物聚合物合成的自动化方法和装置，由此在表面上获得二维基质或生物聚合物阵列。 形式为厚板（12）的施加器（10），其具有在开口腔或通道（14）形式的至少一个空腔的表面（16）中，用于将试剂施加到表面（56）的表面 固相支撑材料。 涂抹器（10）定位在固体支撑材料（56）上方，具有空腔（14）的表面密封在材料表面上。 对于每个空腔（14），合成试剂依次导入空腔的一端并从空腔的另一端收集。 因此，在载体材料（56）上形成一维生物聚合物阵列，其中阵列的每个元件含有具有相同序列的生物聚合物群。 通过相对于支撑材料（56）线性移动或旋转施加器（10）并执行合成程序来进行另外的生物聚合物的进一步合成，使得另一个一维矩阵或生物聚合物阵列形成在与之前的 阵列。 在两个阵列的每个重叠区域，形成生物聚合物的细胞，每个细胞包含来自第一阵列的链和来自第二阵列的链。 其结果是在每个离散的细胞上具有不同序列的生物聚合物的二维阵列。 含有不同生物聚合物的离散细胞阵列可用于进行生物样品的杂交反​​应分析。