-
公开(公告)号：US20150122991A1
公开(公告)日：2015-05-07
申请号：US14594319
申请日：2015-01-12
Applicant: FLUIDIGM CANADA INC.
Inventor： RAYMOND JONG
CPC classification number: H01J49/0445 , G01N15/06 , G01N15/10 , G01N35/10 , G01N2015/0065 , G01N2015/1006 , H01J49/045 , H01J49/105
Abstract: In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.
Abstract translation: 在质谱仪或质谱仪中，将元素标记的颗粒的样品从分散体转移到通过载体气溶胶喷雾的气流中，以通过电感耦合等离子体（ICP）源进行雾化和电离。 样品转印装置的结构允许通过使样品喷雾通过减速阶段来使样品总消耗，以使颗粒从其高速膨胀中的喷雾减速。 在减速阶段后，减速的颗粒样品可以通过加速阶段加速和聚焦，以转移到ICP中。 这有效地改善了样品喷雾和ICP之间的颗粒转移。
-
公开(公告)号：US12283471B2
公开(公告)日：2025-04-22
申请号：US17620049
申请日：2020-06-17
Applicant: Fluidigm Canada Inc.
Inventor： Paul Corkum , Alexander Loboda , David M. Rayner
Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.
-
公开(公告)号：US12124020B2
公开(公告)日：2024-10-22
申请号：US17274295
申请日：2019-09-10
Applicant: Fluidigm Canada Inc.
Inventor： Daaf Sandkuijl , Alexander V. Loboda , Adam Carew , Khashayar Askarpour
CPC classification number: G02B21/245 , G01N1/04 , G01N33/543 , G02B21/0032 , G02B21/006 , G02B21/26 , H01J49/0004 , H01J49/0031 , H01J49/105 , G01N2001/045 , G01N2458/15
Abstract: We describe in this application systems and methods for autofocusing in imaging mass spectrometry. The present application describes improvements over current IMS and IMC apparatus and methods through an autofocus component including a plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used, the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so as to reduce the number of unsuccessful autofocus attempts.
-
公开(公告)号：US11099116B2
公开(公告)日：2021-08-24
申请号：US16895009
申请日：2020-06-08
Applicant: Fluidigm Canada Inc.
Inventor： Alexandre Loboda
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
-
公开(公告)号：US10752707B2
公开(公告)日：2020-08-25
申请号：US16506243
申请日：2019-07-09
Applicant: Fluidigm Canada Inc.
Inventor： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
IPC: G01N33/532 , C08F8/42 , C08F8/32 , C08F220/36 , G01N33/58 , C08F8/14 , C08F220/56 , G01N33/574
Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
-
Patent Agency Ranking
56.发明申请公开(公告)号：US20190161790A1
公开(公告)日：2019-05-30
申请号：US16270904
申请日：2019-02-08
Applicant: Fluidigm Canada Inc.
Inventor： Olga Ornatsky
IPC: C12Q1/6825 , G01N33/58 , C12Q1/6816 , G01N33/543 , C12Q1/6841 , G01N33/68 , H01J49/00
Abstract: The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.
-
公开(公告)号：US10241023B2
公开(公告)日：2019-03-26
申请号：US15956403
申请日：2018-04-18
Applicant: Fluidigm Canada Inc.
Inventor： Alexandre Loboda
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
-
58.发明申请公开(公告)号：US20180188264A1
公开(公告)日：2018-07-05
申请号：US15740527
申请日：2016-06-29
Applicant: Fluidigm Canada Inc.
Inventor： Olga Ornatsky
IPC: G01N33/68 , C12Q1/6872 , C12Q1/6818
CPC classification number: G01N33/6851 , C07H21/00 , C07H21/02 , C12N2310/3145 , C12N2310/315 , C12Q1/68 , C12Q1/6818 , C12Q1/6872 , C12Q2565/627 , C12Q2600/16
Abstract: In certain embodiments, a method of detection includes providing cells labeled with a first element tag bound to a target RNA sequence and a second element tag bound to a target protein. The method may further include ionizing a cell, or a portion thereof, to produce an ionized first and second element tag, and simultaneously detecting the ionized first and second element tag. The ionization may be performed by an inductively coupled plasma (ICP) torch. The method may further comprise nebulizing the cells in suspension prior to ionizing. In certain embodiments, the ionized first and second element tag may be detected by time-of-flight mass spectrometry. Additional methods, and systems and reagents for simultaneous detection of RNA and protein by mass spectrometry are also described.
-
公开(公告)号：US09632079B2
公开(公告)日：2017-04-25
申请号：US14894287
申请日：2014-05-22
Applicant: FLUIDIGM CANADA INC.
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Alexander V. Loboda , Olga Ornatsky , Scott D. Tanner
IPC: G01N33/532 , G01N15/10 , H01J49/00 , G01N33/543 , G01N27/62 , G01N33/483 , G01N15/14
CPC classification number: G01N33/54366 , G01N15/1031 , G01N15/1404 , G01N15/1459 , G01N27/622 , G01N27/624 , G01N33/483 , G01N33/532 , G01N33/54313 , G01N2015/1006 , G01N2458/15
Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.
-
公开(公告)号：US09284597B2
公开(公告)日：2016-03-15
申请号：US14327948
申请日：2014-07-10
Applicant: Fluidigm Canada Inc.
Inventor： Olga Ornatsky
IPC: C12Q1/48 , C12Q1/42 , G01N33/569 , G01N33/58 , G01N33/68
CPC classification number: G01N33/6848 , C12Q1/42 , C12Q1/485 , G01N33/569 , G01N33/58 , G01N33/6842 , G01N2333/91205 , G01N2333/916 , G01N2440/14 , G01N2458/15 , G01N2560/00 , Y10S435/975
Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes.
Abstract translation: 提供了涉及翻译后修饰的酶的方法和试剂盒。 该方法采用元素分析法，包括ICP-MS。 这些方法允许通过参与翻译后修饰的酶（包括激酶和磷酸酶酶）方便和准确地分析底物的翻译后修饰。
-
-
-
-
-
-
-
-
-
Search Results
210
records
(took 0.033 seconds)
