公开(公告)号：KR1020140022199A

公开(公告)日：2014-02-24

申请号：KR1020120088465

申请日：2012-08-13

Applicant: 서울대학교산학협력단

Inventor： 조종수 ,  조명행 ,  최윤재

IPC: A61K48/00 ,  A61K47/48 ,  A61P17/00

CPC classification number: A61K48/0041 ,  A61K47/595 ,  A61K47/60 ,  B82Y5/00 ,  C12N15/111

Abstract: The present invention relates to a N-acetylglucosaminide-combined P[Asp(Sper)-b-PEO-GlcNAc] (PASPG) copolymer comprising polyethylene oxide (PEO) and polyaspartamide, a gene transporter including the PASPG copolymer, and a composition for preventing or treating fibrosis. The PASPG copolymer according to the present invention displays a remarkable combining power to the DNA to form a PASPG/DNA compound, wherein the PASPG/DNA compound is able to transport a gene through an endocytosis with a size equal to or less than 200 nm, and has a very low cytotoxicity and excellent transfection efficiency, thereby can be effectively be used as a gene transporter or a composition for preventing or treating fibrosis.

Abstract translation: 本发明涉及包含聚环氧乙烷（PEO）和聚天冬氨酰胺的N-乙酰氨基葡糖结合的P [Asp（Sper）-b-PEO-GlcNAc]（PASPG）共聚物，包含PASPG共聚物的基因转运体， 或治疗纤维化。 根据本发明的PASPG共聚物显示与DNA的显着组合能力以形成PASPG / DNA化合物，其中PASPG / DNA化合物能够通过大小等于或小于200nm的内吞运输基因， 并且具有非常低的细胞毒性和优异的转染效率，从而可以有效地用作基因转运蛋白或用于预防或治疗纤维化的组合物。

公开(公告)号：KR1020140019261A

公开(公告)日：2014-02-14

申请号：KR1020130159707

申请日：2013-12-19

Applicant: 서울대학교산학협력단

Inventor： 최윤재 ,  김형기 ,  백삼열 ,  강상기 ,  이남경 ,  김훈

IPC: A61K38/08 ,  C07K7/06 ,  A61P35/00

CPC classification number: A61K38/08 ,  C07K7/06

Abstract: The present invention relates to tumor stem cell targeting peptide GICT which can selectively recognize and bind malignant undifferentiated brain tumor stem cells, and a diagnosis method of brain tumor using the peptide. The present invention provides a pharmaceutical composition for preventing and treating malignant tumor which contains the tumor stem cell targeting peptide denoted by sequence number 1 as an effective component.

Abstract translation: 本发明涉及可以选择性识别和结合恶性未分化脑肿瘤干细胞的肿瘤干细胞靶向肽GICT，以及使用该肽的脑肿瘤诊断方法。 本发明提供了含有以序列号1表示的肿瘤干细胞靶向肽作为有效成分的恶性肿瘤的预防和治疗药物组合物。

公开(公告)号：KR101356740B1

公开(公告)日：2014-02-05

申请号：KR1020110018414

申请日：2011-03-02

Applicant: 서울대학교산학협력단

Inventor： 최윤재 ,  김형기 ,  백삼열 ,  강상기 ,  이남경 ,  김훈

IPC: C07K7/06 ,  G01N33/68 ,  A61K38/08 ,  A61P35/00

Abstract: 본 발명은 악성 뇌종양 미분화 줄기세포를 선별적으로 인지하여 결합할 수 있는 종양줄기세포 표적펩타이드 GICT 및 그 펩타이드를 이용한 뇌종양 진단 방법에 관한 것이다.

公开(公告)号：KR1020120124191A

公开(公告)日：2012-11-13

申请号：KR1020110041930

申请日：2011-05-03

Applicant: 서울대학교산학협력단

Inventor： 최윤재 ,  조종수 ,  강상기 ,  모아매드아리풀이슬람

IPC: A61K9/50 ,  A61K9/54 ,  A61K9/48 ,  A61K47/36

CPC classification number: A61K9/5073 ,  A61K9/0053 ,  A61K9/5031 ,  A61K9/5036 ,  A61K9/5089 ,  A61K47/34 ,  A61K47/36

Abstract: PURPOSE: A microcapsule for oral administration and a method for manufacturing the same are provided to improve mucosal adhesion and durability. CONSTITUTION: A microcapsule for oral administration contains 40-85 wt% of alginate, 5-20 wt% of poly-L-lysine, 5-20 wt% of alginate with a thiol group, and 5-20 wt% of physiologically active substance. The alginate is denoted by chemical formula 1. A method for preparing the alginate with the thiol group comprises: a step of adding L-cysteine salt to a reaction mixture; a step of adjusting pH of reacted mixture; and a step of performing dialysis of the reaction mixture. The average particle diameter of the microcapsule is 5-5000 um. A method for preparing the microcapsule comprises: a step of suspending the physiologically active substance in saline solution and adding alginate to the suspension; a step of reacting the reaction mixture in a calcium salt solution; a step of reacting the reaction mixture in a poly-L-lysine solution; and a step of coating the reaction mixture with the alginate solution containing thiol groups.

Abstract translation: 目的：提供用于口服的微胶囊及其制造方法，以改善粘膜粘附性和耐久性。 构成：用于口服给药的微胶囊包含40-85重量％的藻酸盐，5-20重量％的聚-L-赖氨酸，5-20重量％的具有硫醇基团的藻酸盐和5-20重量％的生理活性物质 。 藻酸盐由化学式1表示。用硫醇基制备藻酸盐的方法包括：向反应混合物中加入L-半胱氨酸盐的步骤; 调节反应混合物的pH的步骤; 以及进行反应混合物的透析的步骤。 微胶囊的平均粒径为5-5000μm。 制备微胶囊的方法包括：将生理活性物质悬浮在盐水溶液中并向悬浮液中加入藻酸盐的步骤; 使反应混合物在钙盐溶液中反应的步骤; 将反应混合物在聚-L-赖氨酸溶液中反应的步骤; 以及用含有硫醇基团的藻酸盐溶液涂覆反应混合物的步骤。

公开(公告)号：KR1020120114468A

公开(公告)日：2012-10-17

申请号：KR1020110031979

申请日：2011-04-07

Applicant: 서울대학교산학협력단

Inventor： 최윤재 ,  김형기 ,  윤금룡 ,  김훈 ,  김준겸

IPC: C12N5/0775 ,  A61K35/28

CPC classification number: C12N5/0668 ,  A61K35/28

Abstract: PURPOSE: A mesenchymal stem cell in which endostatin and carboxyl esterase are concurrently over expressed and preventing, treating or recurrence suppression method of glioma by using the same are provided to drastically prevent carcinogenesis and recurrence. CONSTITUTION: In a mesenchymal stem cell, endostatin and carboxyl esterase are concurrently over expressed. A preventing, treating or recurrence suppression method of glioma includes a step of injecting the mesenchymal stem cell to all mammals except for human. A manufacturing method of human amniotic fluid-derived mesenchymal stem cell(AF-MSC) comprises the following steps: establishing positive number of primary cell cultivation in a minimum essential medium in which fetal bovine serum(ES-FBS) having 15% of embryonic stem cell, 2mM L-glutamine and 1% of penicillin streptomycin are contained, and 18% Chang culture medium B and 2% Chang culture medium C are supplemented; collecting cells by trypsinization for selective cultivation of AF-MSC; plating in DMEM low dextrose broth supplemented with 10% of FBS, 1% of penicillin streptomycin, 2mM L-glutamine and 4ng/mL basic fibroblast growth factor (bFGF: R&D); and obtaining morphologic interspecies population of the AF-MSC after second round of the subculture.

Abstract translation: 目的：提供内皮抑素和羧基酯酶同时过表达的间充质干细胞，通过使用胶质瘤预防，治疗或复发抑制方法，大幅度预防癌变和复发。 构成：在间充质干细胞中，内皮抑素和羧基酯酶同时过度表达。 胶质瘤的预防，治疗或复发抑制方法包括将间充质干细胞注射到除人之外的所有哺乳动物的步骤。 人类羊水间充质干细胞（AF-MSC）的制备方法包括以下步骤：在具有15％的胚胎干细胞的胎牛血清（ES-FBS）的最小必需培养基中建立阳性数量的原代细胞培养 细胞，2mM L-谷氨酰胺和1％青霉素链霉素，并补充18％Chang培养基B和2％Chang培养基C; 通过胰蛋白酶消化来收集细胞以选择性培养AF-MSC; 在补充有10％FBS，1％青霉素链霉素，2mM L-谷氨酰胺和4ng / mL碱性成纤维细胞生长因子（bFGF：R＆D）的DMEM低葡萄糖肉汤中电镀; 并在第二轮传代培养后获得AF-MSC的形态种间群体。

公开(公告)号：KR101132412B1

公开(公告)日：2012-04-03

申请号：KR1020090041161

申请日：2009-05-12

Applicant: 서울대학교산학협력단

Inventor： 조종수 ,  최윤재 ,  유미경 ,  강상기

IPC: A61K9/16 ,  A61K47/36 ,  A61K47/42 ,  A61P37/00

Abstract: PURPOSE: A chitosan nanoaprticle immobilized with M cell targeting peptide and a carrier for transferring oral vaccine containing the same are provided to use as a novel carrier for transferring oral vaccine. CONSTITUTION: A method for chitosan nanoparticle immobilized with M cell targeting peptide comprises: a step of conjugating M cell targeting peptide to water soluble chitosan to obtain M cell targeting peptide-water soluble chitosan; a step of adding tripolyphosphate(TPP) solution to the peptide-water soluble chitosan solution containing Tween 80; a step of performing sonication; and a step of centrifuging solution. The M cell targeting peptide has CKSYHPLSC sequence of sequence number 1.