公开(公告)号：CA2641446C

公开(公告)日：2016-06-07

申请号：CA2641446

申请日：2007-02-05

Applicant: HARVARD COLLEGE

Inventor： PARKER KEVIN KIT ,  FEINBERG ADAM W ,  WHITESIDES GEORGE M ,  SHEVKOPLYAS SERGEY S ,  FEIGEL ALEXANDER

IPC: C12N11/02 ,  A61L27/38 ,  C12N5/07 ,  C12N5/071 ,  C12N5/077 ,  C12Q1/02

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

公开(公告)号：AU2014225893A1

公开(公告)日：2015-09-17

申请号：AU2014225893

申请日：2014-03-04

Applicant: HARVARD COLLEGE

Inventor： KWOK SEN W ,  MORIN STEPHEN A ,  MOSADEGH BOBAK ,  SO JU-HEE ,  SHEPHERD ROBERT F ,  WHITESIDES GEORGE M

IPC: B25J9/14

Abstract: Reconfigurable soft robotic actuators with hard components are described. Magnetic attraction is used to couple flexible molded bodies capable of actuation upon pressurization with other flexible molded bodies and/or with hard components (e.g., frames and connectors) to form a seal for fluidic communication and cooperative actuation. Pneumatic de-coupling chambers built into the hard components to de-couple the hard components from the magnetically-coupled soft molded bodies are described. The use of magnetic self-alignment coupling and pneumatic de-coupling allows for the remote assembly and disassembly of complex structures involving hard and soft components. The magnetic coupling allows for rapid, reversible reconfiguration of hybrid soft-hard robots for repair, testing new designs, and carrying out new tasks.

公开(公告)号：AU2013334747A1

公开(公告)日：2015-04-16

申请号：AU2013334747

申请日：2013-10-22

Applicant: HARVARD COLLEGE

Inventor： MOSADEGH BOBAK ,  WHITESIDES GEORGE M

IPC: B25J9/14

Abstract: A pneumatic controller for controllably providing pressurized gas to a target location is disclosed. The pneumatic controller can include an elastomeric manifold comprising a body and a first membrane coupled to a lower portion of the body. The body and the first membrane can form a first integrated channel having a first inlet, a first outlet, and an exhaust, and the first integrated channel is configured to receive pressurized gas at a first pressure at the first inlet and provide the pressurized gas to the first outlet. The body also has a sufficient stiffness to withstand an elevated pressure of the pressurized gas. The pneumatic controller can also include an actuator configured to change the first membrane from a first configuration to a second configuration to control a flow of the pressurized gas in the first integrated channel.

公开(公告)号：CA2788113A1

公开(公告)日：2011-08-11

申请号：CA2788113

申请日：2011-02-03

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  MIRICA KATHERINE A ,  MARTINEZ ANDRES W ,  CHENG CHAO-MIN ,  PHILLIPS SCOTT T ,  MASCARENAS MONICA ,  LIU XINYU ,  LI XIUJUN

IPC: B01L3/00

Abstract: The disclosure provides low cost, portable three-dimensional devices for performing multiplexed assays. The devices comprise at least two substantially planar layers disposed in parallel planes, wherein one of the layers is movable relative to each other parallel to the planes to permit the establishment of fluid flow communication serially between the two layers.

公开(公告)号：CA2329412C

公开(公告)日：2010-09-21

申请号：CA2329412

申请日：1999-04-20

Applicant: HARVARD COLLEGE ,  MASSACHUSETTS INST TECHNOLOGY

Inventor： JACKMAN REBECCA J ,  DUFFY DAVID C ,  WHITESIDES GEORGE M ,  VAETH KATHLEEN M ,  JENSEN KLAVS F

IPC: B41C1/14 ,  G03F7/00 ,  B05D1/32 ,  G03F1/00 ,  G03F7/12 ,  H01L27/32 ,  H01L51/50 ,  H05B33/10

Abstract: An elastomeric mask is provided that allows deposition of a variety of materials through mask openings. The mask seals effectively against substrate surfaces, allowing simple deposition from fluid phase, gas phase, and the like or removal of material using gaseous or liquid etchants. The mask then can be simply peeled from the surface of the substrate leaving the patterned material behind. Multi-layered mask techniques are described in which openings in an upper mask allow selected openings of a lower mask to remain unshielded, while other openings of the lower mask are shielded. A first deposition step, followed by re-orientation of the upper mask to expose a different set of lower mask openings, allows selective deposition of different materials in different openings of the lower mask. Pixelated organic electroluminescent devices are provided via the described technique.

公开(公告)号：CA2754577A1

公开(公告)日：2010-09-10

申请号：CA2754577

申请日：2010-03-08

Applicant: HARVARD COLLEGE

Inventor： NIE ZHIHONG ,  NIJHUIS CHRISTIAN ,  CHEN XIN ,  MARTINEZ ANDRES W ,  NAROVLYANSKY MAX ,  WHITESIDES GEORGE M

IPC: B01L3/00

Abstract: Microfluidic, electrochemical devices are described. The microfluidic, electrochemical device comprises one or more electrode(s) on a substrate and a patterned porous, hydrophilic layer having a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic channels within the patterned porous, hydrophilic layer, wherein the hydrophilic channel(s) comprises a hydrophilic region which is in fiuidic communication with the electrode(s). In some embodiments, the electrodes comprise a working electrode, a counter electrode, and a reference electrode. In some embodiments, the microfluidic, electrochemical device further comprises a fluid sink. The method of assembling the microfluidic, electrochemical device is described. The method of using the device for electrochemical analysis of one or more analytes is also described.

公开(公告)号：CA2564211C

公开(公告)日：2010-08-03

申请号：CA2564211

申请日：2005-01-26

Applicant: HARVARD COLLEGE

Inventor： SIA SAMUEL K ,  LINDER VINCENT ,  WHITESIDES GEORGE M

IPC: B01J4/02 ,  B01L3/00

Abstract: Method and device for storing and/or delivering fluids, wherein at least a first and a second fluid, such as chemical or biochemical reagents or rinse solutions, are maintained separately from each other in a common vessel and transferred in series from the vessel to a reaction site to carry out a predetermined chemical or biochemical reaction. Separation may be achieved by interposing a third fluid, e.g., a gaseous fluid plug, between the first and second fluids.

公开(公告)号：AU2003253751B2

公开(公告)日：2009-10-08

申请号：AU2003253751

申请日：2003-06-30

Applicant: HARVARD COLLEGE ,  GOVERNING COUNCIL UNIV TORONTO

Inventor： LINK DARREN ROY ,  WHITESIDES GEORGE M ,  BONTOUX NATHALIE ,  STONE HOWARD A ,  DILUZIO WILLOW ,  GITLIN IRINA ,  KUMACHEVA EUGENIA ,  GARSTECKI PIOTR ,  ANNA SHELLY L ,  WEITZ DAVID A

IPC: B01F25/46 ,  B01L3/00 ,  B05B7/04

Abstract: A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-availabe, inexpensive material using simple techniques.

公开(公告)号：CA2719320A1

公开(公告)日：2009-10-01

申请号：CA2719320

申请日：2009-03-27

Applicant: HARVARD COLLEGE

Inventor： CARRILHO EMANUEL ,  MARTINEZ ANDRES W ,  MIRICA KATHERINE A ,  PHILLIPS SCOTT T ,  SIEGEL ADAM C ,  WILEY BENJAMIN ,  WHITESIDES GEORGE M

IPC: G01N35/00 ,  G01N33/48

Abstract: Three-dimensional microfluidic devices including by a plurality of patterned porous, hydrophilic layers and a fluid-impermeable layer disposed between every two adjacent patterned porous, hydrophilic layers are described. Each patterned porous, hydrophilic layer has a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic regions within the patterned porous, hydrophilic layer. The fluid-impermeable layer has openings which are aligned with at least part of the hydrophilic region within at least one adjacent patterned porous, hydrophilic layer. Microfluidic assay device, microfluidic mixer, microfluidic flow control device are also described.

公开(公告)号：AU2009228091A1

公开(公告)日：2009-10-01

申请号：AU2009228091

申请日：2009-03-27

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  CARRILHO EMANUEL ,  MIRICA KATHERINE A ,  SIEGEL ADAM C ,  PHILLIPS SCOTT T ,  MARTINEZ ANDRES W ,  WILEY BENJAMIN

IPC: G01N35/00 ,  G01N33/48

Abstract: Three-dimensional microfluidic devices including by a plurality of patterned porous, hydrophilic layers and a fluid-impermeable layer disposed between every two adjacent patterned porous, hydrophilic layers are described. Each patterned porous, hydrophilic layer has a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic regions within the patterned porous, hydrophilic layer. The fluid-impermeable layer has openings which are aligned with at least part of the hydrophilic region within at least one adjacent patterned porous, hydrophilic layer. Microfluidic assay device, microfluidic mixer, microfluidic flow control device are also described.