公开(公告)号：AT346297T

公开(公告)日：2006-12-15

申请号：AT03770419

申请日：2003-09-24

Applicant: INTEL CORP

Inventor： SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  YAMAKAWA MINEO ,  BERLIN ANDREW

IPC: C12Q1/68 ,  G01N33/543 ,  G01B7/34

Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

公开(公告)号：AU2003270751A1

公开(公告)日：2005-04-27

申请号：AU2003270751

申请日：2003-09-15

Applicant: INTEL CORP

Inventor： SU XING ,  BERLIN ANDREW A ,  CHAN SELENA ,  KIRCH STEVEN J ,  KOO TAE-WOONG ,  NEUBAUER GABI ,  RAO VALLURI ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

IPC: C12Q1/68

Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.

公开(公告)号：AU2003278903A1

公开(公告)日：2004-04-19

申请号：AU2003278903

申请日：2003-09-24

Applicant: INTEL CORP

Inventor： YAMAKAWA MINEO ,  BERLIN ANDREW ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG

IPC: C12Q1/68 ,  G01N33/543 ,  G01B7/34

Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

公开(公告)号：AU2003223269A1

公开(公告)日：2003-09-29

申请号：AU2003223269

申请日：2003-03-11

Applicant: INTEL CORP

Inventor： SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO ,  SU XING ,  BERLIN ANDREW ,  KOO TAE-WOONG ,  CHAN SELENA

IPC: G01N21/65 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/68 ,  G01N33/543

Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13 . Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERFS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.