公开(公告)号：MY134398A

公开(公告)日：2007-12-31

申请号：MYPI20033401

申请日：2003-09-09

Applicant: INTEL CORP

Inventor： XING SU ,  SELENA CHAN ,  TAE-WOONG KOO ,  MINEO YAMAKAWA ,  BERLIN ANDREW A

IPC: G01N33/543 ,  C12Q1/68 ,  G01B7/34

Abstract: THE PRESENT METHODS AND APPARATUS CONCERN THE DETECTION AND/OR IDENTIFICATION OF TARGET ANALYTES USING PROBE MOLECULES. IN VARIOUS EMBODIMENTS OF THE INVENTION, THE PROBES OR ANALYTES ARE ATTACHED TO ONE OR MORE CANTILEVERS. BINDING OF A PROBE TO AN ANALYTE RESULTS IN DEFLECTION OF THE CANTILEVER, DETECTED BY A DETECTION UNIT. A COUNTERBALANCING FORCE MAY BE APPLIED TO RESTORE THE CANTILEVER TO ITS ORIGINAL POSITION. THE COUNTERBALANCING FORCE MAY BE MAGNETIC, ELECTRICAL OR RADIATIVE. THE DETECTION UNIT AND THE MECHANISM GENERATING THE COUNTERBALANCING FORCE MAY BE OPERABLY COUPLED TO AN INFORMATION PROCESSING AND CONTROL UNIT, SUCH AS A COMPUTER. THE COMPUTER MAY REGULATE A FEEDBACK LOOP THAT MAINTAINS THE CANTILEVER IN A FIXED POSITION BY BALANCING THE DEFLECTING FORCE AND THE COUNTERBALANCING FORCE. THE CONCENTRATION OF ANALYTES IN A SAMPLE MAY BE DETERMINED FROM THE MAGNITUDE OF THE COUNTERBALANCING FORCE REQUIRED TO MAINTAIN THE CANTILEVER IN A FIXED POSITION.(FIG 1)

公开(公告)号：MY134271A

公开(公告)日：2007-11-30

申请号：MYPI20032186

申请日：2003-06-12

Applicant: INTEL CORP

Inventor： SELENA CHAN ,  BERLIN ANDREW A ,  MINEO YAMAKAWA

IPC: G01J3/44 ,  C12Q1/68 ,  G01N21/65 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553

Abstract: THE METHODS AND APPARATUS (300) DISCLOSED HEREIN CONCERN RAMAN SPECTROSCOPY USING METAL COATED NANOCRYSTALLINE POROUS SILICON SUBSTRATES (240, 340). IN CERTAIN EMBODIMENTS OF THE INVENTION, POROUS SILICON SUBSTRATES (110, 210) MAY BE FORMED BY ANODIC ETCHING IN DILUTE HYDROFLUORIC ACID (150).A THIN COATING OF A RAMAN ACTIVE METAL, SUCH AS GOLD OR SILVER, MAY BE COATED ONTO THE POROUS SILICON (110, 210) BY CATHODIC ELECTROMIGRATION OR ANY KNOWN TECHNIQUE. THE METAL-COATED SUBSTRATE (240, 340) PROVIDES AN EXTENSIVE, METAL RICH ENVIRONMENT FOR SERS, SERRS,HYPER- RAMAN AND/OR CARS RAMAN SPECTROSCOPY. IN CERTAIN EMBODIMENTS OF THE INVENTION, METAL NANOPARTICLES MAY BE ADDED TO THE METAL-COATED SUBSTRATE (240, 340) TO FURTHER ENHANCE THE RAMAN SIGNALS. RAMAN SPECTROSCOPY MAY BE USED TO DETECT, IDENTIFY AND/OR QUANTIFY A WIDE VARIETY OF ANALYTES, USING THE DISCLOSED METHODS AND APPARATUS (300).(FIG 2)

公开(公告)号：AU2003270751A1

公开(公告)日：2005-04-27

申请号：AU2003270751

申请日：2003-09-15

Applicant: INTEL CORP

Inventor： SU XING ,  BERLIN ANDREW A ,  CHAN SELENA ,  KIRCH STEVEN J ,  KOO TAE-WOONG ,  NEUBAUER GABI ,  RAO VALLURI ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

IPC: C12Q1/68

Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.

公开(公告)号：MY139029A

公开(公告)日：2009-08-28

申请号：MYPI20033197

申请日：2003-08-22

Applicant: INTEL CORP

Inventor： SELENA CHAN ,  NARAYAN SUNDARARAJAN ,  BERLIN ANDREW A ,  MINEO YAMAKAWA

IPC: G01N37/00 ,  B01D61/02 ,  B01D61/14 ,  B01D61/18 ,  B01D61/20 ,  B01D69/02 ,  B01D71/02 ,  B01J19/00 ,  B01L3/00 ,  B81B1/00

Abstract: MICROFLUIDIC DEVICE (100) WITH POROUS MEMBRANES FOR MOLECULAR SIEVING, METERING, AND SEPARATION OF ANALYTE FLUIDS. IN ONE ASPECT, A MICROFLUIDIC DEVICE INCLUDES A SUBSTRATE (106) HAVING INPUT AND OUTPUT MICROFLUIDIC CHANNEL SECTIONS (108, 112) SEPARATED BY A POROUS MEMBRANE (202) FORMED INTEGRAL TO THE SUBSTRATE. IN ANOTHER ASPECT, THE POROUS MEMBRANE MAY COMPRISE A THIN MEMBRANE THAT IS SANDWICHED BETWEEN UPPER AND LOWER SUBSTRATE MEMBERS (220, 222). THE MICROFLUIDIC DEVICE MAY INCLUDE ONE OR A PLURALITY OF POROUS MEMBRANES. IN ONE EMBODIMENT, A PLURALITY OF POROUS MEMBRANES (202) HAVING INCREASINGLY SMALLER PORES ARE DISPOSED ALONG PORTIONS OF A MICROFLUIDIC CHANNEL. IN ANOTHER EMBODIMENT, A CASCADING SERIES OF UPPER AND LOWER CHANNELS (204, 206) ARE EMPLOYED, WHEREIN EACH UPPER/LOWER CHANNEL INTERFACE IS SEPARATED BY A RESPECTIVE POROUS MEMBRANE. IN ANOTHER ASPECT, A POROUS MEMBRANE IS ROTATABLY COUPLED TO A SUBSTRATE WITHIN A MICROFLUIDIC CHANNEL VIA A MEMS ACTUATOR (642) TO ENABLE THE POROUS MEMBRANE TO BE POSITIONED IN FILTERING AND PASS-THROUGH POSITIONS.