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    ENGINEERING POLYMERASES AND REACTION CONDITIONS FOR MODIFIED INCORPORATION PROPERTIES
    53.
    发明申请
    ENGINEERING POLYMERASES AND REACTION CONDITIONS FOR MODIFIED INCORPORATION PROPERTIES 审中-公开
    工程聚合物与改性掺入特性的反应条件

    公开(公告)号：WO2010027484A3

    公开(公告)日：2010-06-24

    申请号：PCT/US2009004993

    申请日：2009-09-04

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  PATEL PRANAV ,  KORLACH JONAS ,  BIBILLO ARKADIUSZ ,  BJORNSON KEITH ,  HANES JEREMIAH

    Inventor： PATEL PRANAV ,  KORLACH JONAS ,  BIBILLO ARKADIUSZ ,  BJORNSON KEITH ,  HANES JEREMIAH

    IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25

    CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Q2527/113 ,  C12Q2521/101 ,  C12Q2527/137 ,  C12Q2537/161 ,  C12Q2525/101 ,  C12Q2563/107

    Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.

    Abstract translation: 提供了用于增强核酸模板测序的方法。 还提供了在聚合反应期间增加支化部分的反应条件。 还提供了包含修饰的重组聚合酶的组合物，其表现出比衍生它们的聚合酶的支化部分更高的支化部分。 提供了包含相对于衍生它们的聚合酶显示延迟易位的修饰的重组聚合酶的组合物。 还提供了包含修饰的重组聚合酶的组合物，所述修饰的重组聚合酶在聚合酶的活性位点显示增加的核苷酸或核苷酸类似物停留时间 提供了产生具有上述表型的聚合酶的方法和使用这些聚合酶测序DNA模板或制备DNA的方法。 还提供了用于确定哪些标记的核苷酸在模板依赖性聚合反应期间在位点处掺入的方法和核酸测序系统。

    IMPROVED METHODS, SYSTEMS AND COMPOSITIONS FOR MONITORING ENZYME ACTIVITY AND APPLICATIONS THEREOF
    57.
    发明申请
    IMPROVED METHODS, SYSTEMS AND COMPOSITIONS FOR MONITORING ENZYME ACTIVITY AND APPLICATIONS THEREOF 审中-公开
    用于监测酶活性的改进方法，系统和组合物及其应用

    公开(公告)号：WO2007115205A2

    公开(公告)日：2007-10-11

    申请号：PCT/US2007065687

    申请日：2007-03-30

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  KORLACH JONAS ,  TURNER STEPHEN

    Inventor： KORLACH JONAS ,  TURNER STEPHEN

    IPC: G01C21/32

    CPC classification number: C12Q1/66 ,  C12Q1/42 ,  G01N33/573

    Abstract: Methods of monitoring enzyme mediated reactions, and particularly nucleic acid synthesis reactions such as pyrosequencing methods that employ enzymatic reporter systems. The methods and systems provide elevated signal levels as compared to conventional pyrosequencing processes, and/or mediate de-phasing of sequencing analyses employing pyrosequencing or other "sequencing by synthesis" methods.

    Abstract translation: 监测酶介导的反应的方法，特别是核酸合成反应，例如采用酶报告系统的焦磷酸测序方法。 与常规焦磷酸测序方法相比，该方法和系统提供升高的信号水平，和/或介导使用焦磷酸测序或其他“合成测序”方法进行测序分析的脱相。

    LABELED REACTANTS AND THEIR USES
    58.
    发明专利
    LABELED REACTANTS AND THEIR USES 未知

    公开(公告)号：CA2718404C

    公开(公告)日：2018-04-10

    申请号：CA2718404

    申请日：2009-03-12

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

    Inventor： WEGENER JEFFREY ,  KORLACH JONAS

    IPC: C12Q1/00 ,  G01N33/58

    Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.

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