公开(公告)号：AU2009297145A1

公开(公告)日：2010-04-01

申请号：AU2009297145

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

公开(公告)号：CA2707600C

公开(公告)日：2018-02-20

申请号：CA2707600

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  ROITMAN DANIEL ,  EID JOHN ,  OTTO GEOFF ,  HARDENBOL PAUL ,  FLUSBERG BENJAMIN

IPC: C12Q1/00 ,  C12M1/34 ,  C12M1/40 ,  G01N33/53 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

公开(公告)号：AU2006320414B2

公开(公告)日：2012-10-04

申请号：AU2006320414

申请日：2006-12-01

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： OTTO GEOFFREY ,  EID JOHN ,  TURNER STEPHEN ,  MURPHY DEVON

IPC: G01N33/53 ,  C12N11/02

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.

公开(公告)号：CA2720247C

公开(公告)日：2020-07-14

申请号：CA2720247

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： EID JOHN ,  TURNER STEPHEN ,  DALAL RAVI ,  FLUSBERG BENJAMIN ,  KORLACH JONAS ,  LIN STEVEN ,  FEHR ADRIAN ,  CHRISTIANS FRED ,  EMIG ROBIN

IPC: G01N33/48 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2009251881B2

公开(公告)日：2015-03-26

申请号：AU2009251881

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  CHRISTIANS FRED ,  FEHR ADRIAN ,  FLUSBERG BENJAMIN ,  EMIG ROBIN ,  DALAL RAVI ,  KORLACH JONAS ,  LIN STEVEN ,  EID JOHN

IPC: G01N33/48 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2008331824B2

公开(公告)日：2014-07-24

申请号：AU2008331824

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  EID JOHN ,  OTTO GEOFF ,  KORLACH JONAS ,  ROITMAN DANIEL ,  HARDENBOL PAUL

IPC: G01N33/53 ,  G01N33/535 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.