公开(公告)号：CA2720247C

公开(公告)日：2020-07-14

申请号：CA2720247

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： EID JOHN ,  TURNER STEPHEN ,  DALAL RAVI ,  FLUSBERG BENJAMIN ,  KORLACH JONAS ,  LIN STEVEN ,  FEHR ADRIAN ,  CHRISTIANS FRED ,  EMIG ROBIN

IPC: G01N33/48 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2009251881B2

公开(公告)日：2015-03-26

申请号：AU2009251881

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  CHRISTIANS FRED ,  FEHR ADRIAN ,  FLUSBERG BENJAMIN ,  EMIG ROBIN ,  DALAL RAVI ,  KORLACH JONAS ,  LIN STEVEN ,  EID JOHN

IPC: G01N33/48 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2008331824B2

公开(公告)日：2014-07-24

申请号：AU2008331824

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  EID JOHN ,  OTTO GEOFF ,  KORLACH JONAS ,  ROITMAN DANIEL ,  HARDENBOL PAUL

IPC: G01N33/53 ,  G01N33/535 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

公开(公告)号：AU2011224119B2

公开(公告)日：2014-03-06

申请号：AU2011224119

申请日：2011-09-19

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  KORLACH JONAS

IPC: C12Q1/68 ,  G02B6/00 ,  G02B6/10

Abstract: The present invention relates to optical confinements, method of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.

公开(公告)号：CA2974241A1

公开(公告)日：2010-03-25

申请号：CA2974241

申请日：2009-09-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/17 ,  B82Y20/00 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N21/63 ,  G01N21/77 ,  G02B6/12

Abstract: This specification provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

公开(公告)号：CA2746632C

公开(公告)日：2020-06-30

申请号：CA2746632

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6869

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2737505C

公开(公告)日：2017-08-29

申请号：CA2737505

申请日：2009-09-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/00 ,  G01B9/02 ,  G01N33/483

Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

公开(公告)号：AU2014203354B2

公开(公告)日：2016-06-30

申请号：AU2014203354

申请日：2014-06-20

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/00 ,  G01B9/02 ,  G01N33/483

Abstract: This invention provides substrates for use in various applications, including single molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided. ocdq N"t c 00 (Suonlw)o . o

公开(公告)号：AU2006320739B2

公开(公告)日：2012-03-29

申请号：AU2006320739

申请日：2006-11-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS

IPC: C23C16/00

Abstract: Devices, systems and methods of using same where hybrid substrate materials are provided with a substantially uniform surface to provide uniformity of properties, including interaction with their environments. Uniform surfaces are applied as coatings over, e.g., hybrid metal/silica, metal/polymer, metal/metal surfaces to mask different chemical properties of differing regions of the surface and to afford a protective surface for the hybrid structure.

公开(公告)号：AU2009288696A1

公开(公告)日：2010-03-11

申请号：AU2009288696

申请日：2009-09-04

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： PATEL PRANAV ,  KORLACH JONAS ,  BIBILLO ARKADIUSZ ,  BJORNSON KEITH ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25

Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.