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    Polymer backbone element tags
    61.
    发明授权
    Polymer backbone element tags 有权

    公开(公告)号：US09169335B2

    公开(公告)日：2015-10-27

    申请号：US14659224

    申请日：2015-03-16

    Applicant: FLUIDIGM CANADA INC.

    Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

    IPC: G01N33/544 ,  G01N33/545 ,  G01N33/24 ,  C08F8/32 ,  C08F8/14 ,  C08F8/42

    Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

    POLYMER BACKBONE ELEMENT TAGS
    62.
    发明申请
    POLYMER BACKBONE ELEMENT TAGS 有权
    POLYMER BACKBONE ELEMENT标签

    公开(公告)号：US20150183895A1

    公开(公告)日：2015-07-02

    申请号：US14659224

    申请日：2015-03-16

    Applicant: FLUIDIGM CANADA INC.

    Inventor： Mitchell A. Winnik ,  MARK NITZ ,  VLADIMIR BARANOV ,  XUDONG LOU

    IPC: C08F8/32 ,  C08F8/42 ,  C08F8/14

    CPC classification number: C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

    Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

    Abstract translation: 提供基于新型金属 - 聚合物共轭物的元件标签用于元素分析，包括ICP-MS。 聚合物主链被官能化以不可逆地结合在用户使用之前选择的金属。 该聚合物进一步官能化以连接允许附着于抗体或其它亲和试剂的接头。 聚合物形式允许附加许多拷贝的给定同位素，其线性地提高灵敏度。 金属 - 聚合物缀合物标签使得能够以两种形式进行多重测定：批量测定，其中样品中的平均生物标志物分布是诊断的，以及单细胞格式以区分复杂样品中的罕见（例如患病）细胞（例如， 血液）。

    APPARATUS FOR ELEMENTAL ANALYSIS OF PARTICLES BY MASS SPECTROMETRY
    63.
    发明申请
    APPARATUS FOR ELEMENTAL ANALYSIS OF PARTICLES BY MASS SPECTROMETRY 有权
    用于通过质谱分析颗粒进行元素分析的装置

    公开(公告)号：US20140299763A1

    公开(公告)日：2014-10-09

    申请号：US14309591

    申请日：2014-06-19

    Applicant: Fluidigm Canada Inc.

    Inventor： Alexei Antonov ,  Dmitry Roman Bandura

    IPC: H01J49/00

    CPC classification number: H01J49/0036 ,  H01J49/0027

    Abstract: A mass spectrometer has a particle introduction system and a vaporizer, atomizer, and ionizer configured to produce ions from elements associated with the particle. An ion mass-to-charge ratio analyzer is configured to separate ions according to their mass-to-charge ratio. A detector is positioned to detect at least some of the separated ions. A digital processor is configured to: (a) acquire data from the detector including at least first data in a primary detection group defined to comprise one or more mass-to-charge ratio channels of the mass spectrometer; (b) determine whether or not ions detected during at least one sampling cycle meet at least one selection criterion indicating a presence of a particle in the mass spectrometer; and (c) determine whether or not to use data in a secondary detection group based on whether or not the at least one selection criterion is met.

    Abstract translation: 质谱仪具有粒子引入系统和配置成从与颗粒相关的元件产生离子的蒸发器，雾化器和离子发生器。 离子质荷比分析仪被配置为根据其质荷比分离离子。 定位检测器以检测至少一些分离的离子。 数字处理器被配置为：（a）从检测器采集数据，其中包括被定义为包括质谱仪的一个或多个质荷比通道的初级检测组中的至少第一数据; （b）确定在至少一个采样周期期间检测到的离子是否满足指示质谱仪中颗粒存在的至少一个选择标准; 以及（c）基于是否满足所述至少一个选择标准来确定是否在次级检测组中使用数据。

    SAMPLE ANALYSIS FOR MASS CYTOMETRY
    66.
    发明申请
    SAMPLE ANALYSIS FOR MASS CYTOMETRY 有权

    公开(公告)号：US20220178809A1

    公开(公告)日：2022-06-09

    申请号：US17382764

    申请日：2021-07-22

    Applicant: Fluidigm Canada Inc.

    Inventor： Alexander V. Loboda

    IPC: G01N15/10 ,  H01J49/10 ,  G01N15/14 ,  H01J49/04

    Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.

    LYOPHILIZED ANTIBODY PANEL
    67.
    发明申请
    LYOPHILIZED ANTIBODY PANEL 有权

    公开(公告)号：US20220155308A1

    公开(公告)日：2022-05-19

    申请号：US17439503

    申请日：2020-03-27

    Applicant: Fluidigm Canada Inc.

    Inventor： Stephen K.H. Li ,  Daniel Majonis ,  Vladimir Baranov ,  Olga Ornatsky ,  Bedilu Allo ,  Dmitry Bandura ,  Colin Thom ,  Greg Stelzer ,  Alexander Loboda

    IPC: G01N33/58 ,  G01N1/30 ,  G01N15/10

    Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

    DIRECT IONIZATION IN IMAGING MASS SPECTROMETRY OPERATION
    68.
    发明申请
    DIRECT IONIZATION IN IMAGING MASS SPECTROMETRY OPERATION 有权

    公开(公告)号：US20210391161A1

    公开(公告)日：2021-12-16

    申请号：US17422578

    申请日：2020-01-14

    Applicant: Fluidigm Canada Inc.

    Inventor： Alexander Loboda ,  Daaf Sandkuijl ,  Adam Carew

    IPC: H01J49/00 ,  H01J49/16

    Abstract: As described herein, one or more parameters of a direct ionization imaging mass spectrometer (IMS) may be set to obtain a desired plasma and deliver it to a mass detector. Depending on the application, certain parameters may be predetermined (e.g., a spot size given a desired resolution) and, as described herein, other parameters can be adjusted to obtain the desired plasma properties. Also included is sample preparation suitable for direct ionization IMS and/or other imaging modalities.

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