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    돼지콜레라 바이러스 Ｅｒｎｓ 단백질 및 이에 특이적으로반응하는 단클론항체를 이용한 돼지콜레라 항체 감별진단방법
    61.
    发明公开
    돼지콜레라 바이러스 Ｅｒｎｓ 단백질 및 이에 특이적으로반응하는 단클론항체를 이용한 돼지콜레라 항체 감별진단방법 有权
    SWINE FEVER VIRUS ERNS蛋白质和筛选和检测与其相似的SWINE FEVER病毒抗体的方法

    公开(公告)号：KR1020070116382A

    公开(公告)日：2007-12-10

    申请号：KR1020060050357

    申请日：2006-06-05

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

    Inventor： 송재영 ,  김병한 ,  탁동섭 ,  최은진 ,  임성인 ,  한규하 ,  주후돈 ,  장병식 ,  이현정 ,  박경민

    IPC: C12N15/33 ,  C12N15/09 ,  C12N15/10

    Abstract: A method for screening and detecting a swine fever virus antibody is provided to easily screen and diagnose infected swine fever virus antibody by using a monoclonal antibody specifically reacting with a recombinant swine fever virus Erns protein and secure constant sensitivity regardless of all genotypes of the swine fever virus when using all three kinds of recombinant protein. A recombinant swine fever virus Erns is prepared by expressing a gene encoding three Erns proteins among swine fever viruses having various genotypes using baculovirus. A method for preparing a monoclonal antibody specifically reacting to the Erns comprises the steps of: (a) constructing a swine fever virus LOM strain-derived Erns recombinant gene using a baculovirus expression system; and (b) producing the monoclonal antibody using the recombinant Erns protein. A method for screening and detecting a swine fever virus antibody comprises the steps of: (a) mixing three kinds of Erns recombinant proteins at proper concentration to prepare an antigen cocktail; (b) after diluting the antigen cocktail into a coating buffer solution, pouring it in a plate to be absorbed into the plate; (c) washing the recombinant antigen not-absorbed into the plate to remove it; (d) adding a sample to be tested to the plat to allow the reaction; (e) washing the sample to be tested not specifically bound to the recombinant Erns antigen protein absorbed into the plate to remove it; (f) adding a monoclonal antibody not being bound to the enzyme but being specifically reacted to the Erns protein to allow the reaction; (g) washing the monoclonal antibody not being bound to the recombinant Erns antigen protein absorbed into the plate to remove it; and (h) adding a substrate reacting with the enzyme to determine the swine fever virus infection.

    Abstract translation: 提供了一种筛选和检测猪瘟病毒抗体的方法，通过使用与重组猪瘟病毒Erns蛋白特异性反应的单克隆抗体轻松筛选和诊断感染的猪瘟病毒抗体，无论猪瘟的所有基因型如何 使用全部三种重组蛋白的病毒。 通过使用杆状病毒在具有各种基因型的猪瘟病毒中表达编码三种Erns蛋白质的基因来制备重组猪瘟病毒Erns。 制备与Erns特异性反应的单克隆抗体的方法包括以下步骤：（a）使用杆状病毒表达系统构建猪瘟病毒LOM菌株衍生的Erns重组基因; 和（b）使用重组Erns蛋白产生单克隆抗体。 筛选和检测猪瘟病毒抗体的方法包括以下步骤：（a）以适当浓度混合三种Erns重组蛋白以制备抗原混合物; （b）将抗原混合物稀释到涂布缓冲溶液中后，将其倒入板中吸收到板中; （c）将未吸收的重组抗原洗涤到板中以除去它; （d）将待测试样品加入平板以允许反应; （e）洗涤待测试的样品，未特异性地结合到吸收到板中的重组Erns抗原蛋白质以除去它; （f）加入未结合酶的单克隆抗体，但与Erns蛋白特异性反应以进行反应; （g）洗涤未结合到吸附到板中的重组Erns抗原蛋白的单克隆抗体以除去它; 和（h）加入与酶反应的底物以确定猪瘟病毒感染。

    유전자재조합 바큘로바이러스 및 이에 의한 재조합 돼지전염성위장염바이러스 스파이크 단백질을 이용한 돼지전염성위장염바이러스 항체 검출을 위한 효소면역 검사방법
    62.
    发明授权
    유전자재조합 바큘로바이러스 및 이에 의한 재조합 돼지전염성위장염바이러스 스파이크 단백질을 이용한 돼지전염성위장염바이러스 항체 검출을 위한 효소면역 검사방법 有权
    重组杆状病毒和使用重组TGEV SPIKE蛋白的MACELISA检测特异性抗体对TGEV的检测

    公开(公告)号：KR100267744B1

    公开(公告)日：2000-11-01

    申请号：KR1019980000446

    申请日：1998-01-10

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 탁동섭 ,  권준헌 ,  김병한 ,  송재영 ,  강신영 ,  안수환

    IPC: C12N15/85 ,  C12N15/33

    Abstract: PURPOSE: Provided are a genetic recombinant baculovirus and a recombinant spike protein of transmissible gastroenteritis virus(TGEV) manufactured therefrom. And a monoclonal antibody capture enzyme linked immunosorbent assay for detecting the antibody of TGEV using the same protein is also provided, thereby the antibody of TGEV can be rapidly detected. CONSTITUTION: The recombinant spike protein of TGEV is produced by the steps of: isolating and identifying TGEV from pigs died of diarrhea; analyzing the nucleotide sequence of spike protein of TGEV; inserting the TGEV spike gene into the baculovirus gene to produce a recombinant baculovirus(KFCC-11015); infecting the recombinant baculovirus into SF9 cells to express TGEV spike protein. The antibody of TGEV is produced by inoculating the expressed recombinant TGEV spike protein into Guinea pigs. The monoclonal antibody capture enzyme linked immunosorbent assay using TGEV spike protein that is produced from the recombinant baculovirus(KFCC-11015) in Guinea pigs as an antigen is used in detecting the neutralizing antibody of TGEV.

    Abstract translation: 目的：提供遗传重组杆状病毒和由其制备的传染性胃肠炎病毒（TGEV）的重组穗蛋白。 还提供了使用相同蛋白质检测TGEV抗体的单克隆抗体捕获酶联免疫吸附测定法，从而可以快速检测TGEV抗体。 构成：TGEV的重组穗蛋白是通过以下步骤制备的：从腹泻死亡的猪中分离和鉴定TGEV; 分析TGEV刺突蛋白的核苷酸序列; 将TGEV刺突基因插入杆状病毒基因以产生重组杆状病毒（KFCC-11015）; 将重组杆状病毒感染到SF9细胞中以表达TGEV穗蛋白。 通过将表达的重组TGEV穗蛋白接种到豚鼠中来产生TGEV抗体。 使用从作为抗原的豚鼠中的重组杆状病毒（KFCC-11015）产生的TGEV尖峰蛋白的单克隆抗体捕获酶联免疫吸附测定用于检测TGEV的中和抗体。

    구제역바이러스 소독용 조성물
    68.
    发明公开
    구제역바이러스 소독용 조성물 无效
    消毒口蹄疫病毒的成分

    公开(公告)号：KR1020150030525A

    公开(公告)日：2015-03-20

    申请号：KR1020130109910

    申请日：2013-09-12

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 이광녕 ,  김수미 ,  박종현 ,  고영준 ,  이향심 ,  신연경 ,  탁동섭 ,  홍장관 ,  서민구 ,  이경기 ,  정진호 ,  김병한

    IPC: A01N37/02 ,  A61L2/16 ,  C09K3/18 ,  C11D11/00

    CPC classification number: A01N37/02 ,  A61L2/16 ,  C09K3/18 ,  C11D11/0023

    Abstract: 본 발명은 -20℃ 이하에서 동결되지 않으면서, 구제역바이러스에 대한 소독효과를 갖는 구제역바이러스 소독용 조성물에 관한 것으로, 구연산 또는 구연산을 유효성분으로 함유하는 소독제, 및 동결방지제를 포함한다.
    본 발명의 구제역바이러스 소독용 조성물에서, 상기 동결방지제는 메탄올을 유효성분으로 함유하는 자동차유리 세척제, 에틸렌글리콜, 프로필렌글리콜, 염화나트륨, 염화칼슘 및 에탄올로 이루어진 군으로부터 선택되는 1종 이상일 수 있다.

    Abstract translation: 本发明涉及用于消毒口蹄疫病毒的组合物，其在-20℃下不会冷冻并对口蹄疫病毒具有消毒作用。 组合物包含柠檬酸或包含柠檬酸作为活性成分的消毒剂和防冻剂。 防冻剂可以是选自包括甲醇作为活性成分的挡风玻璃洗涤液，乙二醇，丙二醇，氯化钠，氯化钙和乙醇的一种或多种。

    돼지전염성위장염바이러스, 유행성설사바이러스 및로타바이러스의 혼합생백신 및 이를 이용한 백신접종방법
    70.
    发明授权
    돼지전염성위장염바이러스, 유행성설사바이러스 및로타바이러스의 혼합생백신 및 이를 이용한 백신접종방법 有权
    돼지전염성위장염바이러스，유행성설사바이러스및로타바이러스의혼합생백신및이를이용한백신접종방돼

    公开(公告)号：KR100737434B1

    公开(公告)日：2007-07-09

    申请号：KR1020060022530

    申请日：2006-03-10

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 탁동섭 ,  김성희 ,  권준헌 ,  조수동 ,  양동군 ,  김병한

    IPC: A61K39/225 ,  A61K39/12

    Abstract: A mixed live vaccine of transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus, and a vaccination method by using the same live vaccine are provided to prevent diarrhea of pigs at one time, reduce vaccination time, and minimize titer loss of attenuated viruses by using trehalose. The mixed live vaccine is prepared by mixing a transmissible gastroenteritis virus(KCTC 10889BP), a porcine epidemic diarrhea virus(KCTC 10891BP) and a porcine rotavirus(KCTC 10890BP) in a weight ratio of 1:1:1, and adding a trehalose phosphate glutamate gelatin mixture. The mixed live vaccine is vaccinated by vaccinating a mother pig with the mixed live vaccine one time, and vaccinating the first vaccinated mother pig with the vaccine of transmissible gastroenteritis virus(KCTC 10889BP) and porcine epidemic diarrhea virus(KCTC 10891BP) two times.

    Abstract translation: 提供传染性胃肠炎病毒，猪流行性腹泻病毒和猪轮状病毒的混合活疫苗和使用相同活疫苗的疫苗接种方法，以一次预防猪腹泻，减少疫苗接种时间，并通过以下方式使减毒病毒的滴度损失最小化 使用海藻糖。 混合活疫苗通过混合重量比为1:1:1的传染性胃肠炎病毒（KCTC 10889BP），猪流行性腹泻病毒（KCTC 10891BP）和猪轮状病毒（KCTC 10890BP），并加入海藻糖磷酸酯 谷氨酸明胶混合物。 将混合活疫苗一次用混合活疫苗接种疫苗接种混合活疫苗，并用传染性胃肠炎病毒（KCTC 10889BP）和猪流行性腹泻病毒（KCTC 10891BP）疫苗接种第一次接种疫苗的母猪两次。

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