Abstract:
본 발명은 GST-구제역바이러스 3D유전자 융합단백질로서 발현벡터의 단백질인 GST와 구제역바이러스유전자 발현단백질인 3D가 융합된 형태의 단백질이며 GST항체를 이용한 구제역 항체의 검출방법에 관한 것이다. 따라서, 본 발명의 목적은 신규한 GST-구제역바이러스 3D유전자 융합단백질 발현벡터 및 구제역 혈청형 항체를 동시에 검출함으로써 안전하고 간편하며 신속하게 구제역 항체를 검출할 수 있는 새로운 진단방법을 제공함에 있다. 본 발명에 의한 진단방법은 효소면역법의 원리를 이용함으로써 대량의 시료를 신속하게 검색할 수 있고, 인공적으로 만든 단백질을 이용함으로써 진단 항원의 안전성이 확보될 수 있으며, 구제역바이러스 4가지 혈청형의 항체를 동시에 검출할 수 있게 됨으로써 혈청형별 항체검사를 수행해야 하는 번거로움을 해소할 수 있는 장점이 있다.
Abstract:
PURPOSE: Provided is a device for detecting antibody of Aujeszky's disease. And a method for diagnosing Aujeszky virus more rapidly and correctly using the same is also provided. CONSTITUTION: The device for detecting antibody of Aujeszky's disease is composed of; i) a nitrocellulose membrane (5), absorption pad (3), supporting plate (6), and soaking section (4). The method for detecting antibodies of Aujeszky virus is comprised of the following steps of: i) spreading antigens (1) of Aujeszky virus and anti-swine IgG (2) evenly on the nitrocellulose membrane (5); and ii) reacting the antigens (1) and IgG (2) with antibodies of Aujeszky virus in the soaking section (4), which shows different colors depending on the existence of antibodies.
Abstract:
PURPOSE: Recombinant porcine Aujesky's disease virus provides safe vector vaccine against porcine diseases and can be used to produce effective vaccine against other diseases if antigen of other virus is introduced in it. CONSTITUTION: Recombinant porcine Aujesky's disease virus is useful to prepared a vaccine vector. The vector is constructed by; deletion of K gene; insertion of IL-2 gene; deletion of gI gene; insertion of beta-galactosidase gene as a maker gene; and insertion of foreign gene coding antigen for virus pathogen. Thereby, immunity is improved.
Abstract:
PURPOSE: Provided are a fluorescent vector, which is used for the production of vaccine of porcine Aujesky's disease and a method for selecting recombination virus using the same. CONSTITUTION: A fluorescent vector pHAVE3 is prepared by amplifying pVIII gene corresponding to 5' region of E3 gene of human Adenovirus type II, a partial sequence(27100-28811bp) having 1,712base, before open reading frame of E3 gene being 19kd, a partial sequence(30,851-32,760bp) having 1,910base, after E3-2 polyadenylation signal corresponding to 3' region of E3 gene; cloning them into SpeI of plasmid pGEMT; and inserting BamH I restriction site